This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hano, T. Articles by Rho, J. Search for Related Content PubMed PubMed Citation Articles by Hano, T. Articles by Rho, J.

Hypertension, Vol 13, 250-255, Copyright © 1989 by American Heart Association

ARTICLES

Norepinephrine release and reuptake by hypothalamic synaptosomes of spontaneously hypertensive rats

T Hano, Y Jeng and J Rho
Department of Clinical Pharmacology, School of Medicine, University of Southern California, Los Angeles 90033.

We compared the overflow of endogenous norepinephrine during electrical field stimulation, the norepinephrine content, and the rate of initial neuronal uptake of [3H]norepinephrine in synaptosomes isolated from hypothalamus and brainstem of spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats at 7 and 13 weeks of age. The synaptosomes of two rats, a SHR and a WKY rat control, were simultaneously processed and subjected to the same electrical field stimulation. The overflow of endogenous norepinephrine during electrical stimulation (2 Hz, 2 minutes) in the hypothalamic synaptosomes of 7-week-old SHR was significantly greater, whereas the overflow of 13-week-old SHR was equivalent to the age-matched WKY rat. The norepinephrine content of synaptosomes was about the same in SHR and age-matched controls. There was also significantly enhanced [3H]norepinephrine uptake in the hypothalamic synaptosomes of young SHR, but neither the hypothalamic nor the brainstem samples of 13-week-old SHR showed any significant difference in their rate of [3H]norepinephrine uptake. These data are similar to those we observed (unpublished observations) in perfused mesenteric artery system in which norepinephrine release was significantly elevated during periarterial nerve stimulation only in young SHR. Thus, these results suggest that a parallel enhancement of norepinephrine release in hypothalamus with that of peripheral nervous system may play an important role during development of hypertension in young SHR.

This article has been cited by other articles:

				S. Kasparov and A. G. Teschemacher Altered central catecholaminergic transmission and cardiovascular disease Exp Physiol, June 1, 2008; 93(6): 725 - 740. [Abstract] [Full Text] [PDF]

				A. C. Ashton and Y. A. Ushkaryov Properties of Synaptic Vesicle Pools in Mature Central Nerve Terminals J. Biol. Chem., November 4, 2005; 280(44): 37278 - 37288. [Abstract] [Full Text] [PDF]

				T. Kishi, Y. Hirooka, K. Ito, K. Sakai, H. Shimokawa, and A. Takeshita Cardiovascular Effects of Overexpression of Endothelial Nitric Oxide Synthase in the Rostral Ventrolateral Medulla in Stroke-Prone Spontaneously Hypertensive Rats Hypertension, February 1, 2002; 39(2): 264 - 268. [Abstract] [Full Text] [PDF]

				T. Nagayama, T. Matsumoto, M. Yoshida, M. Suzuki-Kusaba, H. Hisa, T. Kimura, and S. Satoh Role of cholinergic receptors in adrenal catecholamine secretion in spontaneously hypertensive rats Am J Physiol Regulatory Integrative Comp Physiol, October 1, 1999; 277(4): R1057 - R1062. [Abstract] [Full Text] [PDF]

				M. Yin and A. F. Sved Role of {gamma}-Aminobutyric Acid B Receptors in Baroreceptor Reflexes in Hypertensive Rats Hypertension, June 1, 1996; 27(6): 1291 - 1298. [Abstract] [Full Text]