Hypertension, Vol 13, 589-597, Copyright © 1989 by American Heart Association
V Hadrava, J Tremblay and P Hamet
Comparative studies have shown that cultured vascular smooth muscle cells
from spontaneously hypertensive rats (SHR) proliferate to a higher cell
number, grow to a greater density, and have greater specific growth rate,
particularly at a higher saturation density, than those of the normotensive
Wistar-Kyoto (WKY) control rats. The growth difference was not due to
varying cell survival nor to attachment ability after passage. The degree
of DNA synthesis was estimated by [3H]thymidine incorporation into newly
synthesized DNA. [3H]thymidine uptake increased with escalating
concentrations of calf serum and reached a plateau at 5% calf serum in WKY
rats, whereas an excessive, continuous rise was observed in SHR with up to
a 20% concentration. [3H]thymidine incorporation into newly synthesized DNA
was tested after stimulation by platelet-derived growth factor and
epidermal growth factor. A significantly higher amount of newly synthesized
DNA in vascular smooth muscle cells from SHR was noted when the cells were
stimulated by platelet-derived growth factor or epidermal growth factor
alone, and their simultaneous addition did not significantly change the 50%
effective concentration but heightened the maximal response. These data
provide evidence of increased aortic smooth muscle cell proliferation from
aortas of SHR after mitogen stimulation and suggest a defect in growth
stimulatory-inhibitory control.
ARTICLES
Abnormalities in growth characteristics of aortic smooth muscle cells in spontaneously hypertensive rats
Clinical Research Institute of Montreal, Quebec, Canada.
This article has been cited by other articles:
 |
|
 |
|
  Y. Yang, K. K. Parsons, L. Chi, S. M. Malakauskas, and T. H. Le Glutathione S-Transferase-{micro}1 Regulates Vascular Smooth Muscle Cell Proliferation, Migration, and Oxidative Stress Hypertension, December 1, 2009; 54(6): 1360 - 1368. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. Martin and T. Jo Genetic Differences in Airway Smooth Muscle Function Proceedings of the ATS, January 1, 2008; 5(1): 73 - 79. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Gros, Q. Ding, J. Chorazyczewski, J. Andrews, J. G. Pickering, R. A. Hegele, and R. D. Feldman The Impact of Blunted beta-Adrenergic Responsiveness on Growth Regulatory Pathways in Hypertension Mol. Pharmacol., January 1, 2006; 69(1): 317 - 327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Dutil, V. Eliopoulos, E.-L. Marchand, A. M. Devlin, J. Tremblay, K. Prithiviraj, P. Hamet, A. Migneault, D. deBlois, and A. Y. Deng A quantitative trait locus for aortic smooth muscle cell number acting independently of blood pressure: implicating the angiotensin receptor AT1B gene as a candidate Physiol Genomics, May 11, 2005; 21(3): 362 - 369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-H. Lin, N. Fukuda, X.-Q. Jin, E.-H. Yao, T. Ueno, M. Endo, S. Saito, K. Matsumoto, and H. Mugishima Complement 3 Is Involved in the Synthetic Phenotype and Exaggerated Growth of Vascular Smooth Muscle Cells From Spontaneously Hypertensive Rats Hypertension, July 1, 2004; 44(1): 42 - 47. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. R. Polte, G. S. Eichler, N. Wang, and D. E. Ingber Extracellular matrix controls myosin light chain phosphorylation and cell contractility through modulation of cell shape and cytoskeletal prestress Am J Physiol Cell Physiol, March 1, 2004; 286(3): C518 - C528. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Ragolia, T. Palaia, E. Paric, and J. K. Maesaka Prostaglandin D2 Synthase Inhibits the Exaggerated Growth Phenotype of Spontaneously Hypertensive Rat Vascular Smooth Muscle Cells J. Biol. Chem., June 6, 2003; 278(24): 22175 - 22181. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Devlin, N. Solban, S. Tremblay, J. Gutkowska, W. Schurch, S. N. Orlov, R. Lewanczuk, P. Hamet, and J. Tremblay HCaRG is a novel regulator of renal epithelial cell growth and differentiation causing G2M arrest Am J Physiol Renal Physiol, April 1, 2003; 284(4): F753 - F762. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-Z. Su, N. Fukuda, X.-Q. Jin, Y.-M. Lai, R. Suzuki, Y. Tahira, H. Takagi, Y. Ikeda, K. Kanmatsuse, and H. Miyazaki Effect of AT2 Receptor on Expression of AT1 and TGF-{beta} Receptors in VSMCs from SHR Hypertension, December 1, 2002; 40(6): 853 - 858. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Taurin, V. Seyrantepe, S. N. Orlov, T.-L. Tremblay, P. Thibault, M. R. Bennett, P. Hamet, and A. V. Pshezhetsky Proteome Analysis and Functional Expression Identify Mortalin as an Antiapoptotic Gene Induced by Elevation of [Na+]i/[K+]i Ratio in Cultured Vascular Smooth Muscle Cells Circ. Res., November 15, 2002; 91(10): 915 - 922. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. E. Woodard, J. A. Rosado, and J. Brown Expression and control of C-type natriuretic peptide in rat vascular smooth muscle cells Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2002; 282(1): R156 - R165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Hamet, N. Thorin-Trescases, P. Moreau, P. Dumas, B.-S. Tea, D. deBlois, V. Kren, M. Pravenec, J. Kunes, Y. Sun, et al. Workshop: Excess Growth and Apoptosis : Is Hypertension a Case of Accelerated Aging of Cardiovascular Cells? Hypertension, February 1, 2001; 37(2): 760 - 766. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Teng, N. Fukuda, W.-Y. Hu, M. Nakayama, H. Kishioka, and K. Kanmatsuse DNA-RNA chimeric hammerhead ribozyme to transforming growth factor-{beta}1 mRNA inhibits the exaggerated growth of vascular smooth muscle cells from spontaneously hypertensive rats Cardiovasc Res, October 1, 2000; 48(1): 138 - 147. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Devlin, J. S. Clark, J. L. Reid, and A. F. Dominiczak DNA Synthesis and Apoptosis in Smooth Muscle Cells From a Model of Genetic Hypertension Hypertension, July 1, 2000; 36(1): 110 - 115. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. N. Orlov, S. Taurin, N. Thorin-Trescases, N. O. Dulin, J. Tremblay, and P. Hamet Inversion of the Intracellular Na+/K+ Ratio Blocks Apoptosis in Vascular Smooth Muscle Cells by Induction of RNA Synthesis Hypertension, May 1, 2000; 35(5): 1062 - 1068. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.-C. Desfaits, J. Raymond, and J. P. Muizelaar Growth Factors Stimulate Neointimal Cells In Vitro and Increase the Thickness of the Neointima Formed at the Neck of Porcine Aneurysms Treated by Embolization • Editorial Comment Stroke, February 1, 2000; 31(2): 498 - 507. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Zacour and J. G. Martin Protein kinase C is involved in enhanced airway smooth muscle cell growth in hyperresponsive rats Am J Physiol Lung Cell Mol Physiol, January 1, 2000; 278(1): L59 - L67. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Mathew, N. Y.-T. Fan, N. Yuan, P. N. Chander, M. H. Gewitz, and C. T. Stier Jr. Inhibition of NOS enhances pulmonary vascular changes in stroke-prone spontaneously hypertensive rats Am J Physiol Lung Cell Mol Physiol, January 1, 2000; 278(1): L81 - L89. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Fukuda, C. Satoh, W.-Y. Hu, M. Soma, A. Kubo, H. Kishioka, Y. Watanabe, Y. Izumi, and K. Kanmatsuse Production of Angiotensin II by Homogeneous Cultures of Vascular Smooth Muscle Cells From Spontaneously Hypertensive Rats Arterioscler Thromb Vasc Biol, May 1, 1999; 19(5): 1210 - 1217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. N. Orlov, N. C. Adragna, V. A. Adarichev, and P. Hamet Genetic and biochemical determinants of abnormal monovalent ion transport in primary hypertension Am J Physiol Cell Physiol, March 1, 1999; 276(3): C511 - C536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-J. Champagne, P. Dumas, S. N. Orlov, M. R. Bennett, P. Hamet, and J. Tremblay Protection Against Necrosis but Not Apoptosis by Heat-Stress Proteins in Vascular Smooth Muscle Cells : Evidence for Distinct Modes of Cell Death Hypertension, March 1, 1999; 33(3): 906 - 913. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Fukuda, W.-Y. Hu, A. Kubo, M. Endoh, H. Kishioka, C. Satoh, M. Soma, Y. Izumi, and K. Kanmatsuse Abnormal Regulation of Transforming Growth Factor-ß Receptors on Vascular Smooth Muscle Cells From Spontaneously Hypertensive Rats by Angiotensin II Hypertension, February 1, 1998; 31(2): 672 - 677. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-M. Lee, K. Y. Tsai, N. Wang, and D. E. Ingber Extracellular matrix and pulmonary hypertension: control of vascular smooth muscle cell contractility Am J Physiol Heart Circ Physiol, January 1, 1998; 274(1): H76 - H82. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. N. Orlov, S. L. Aksentsev, N. I. Pokudin, J. Tremblay, and P. Hamet Na+/H+ Exchange in Vascular Smooth Muscle Cells Is Controlled by GTP-Binding Proteins Hypertension, January 1, 1998; 31(1): 259 - 265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Liu, E. Espinosa, B. S. Oemar, and T. F. Luscher Bimodal Effects of Angiotensin II on Migration of Human and Rat Smooth Muscle Cells : Direct Stimulation and Indirect Inhibition Via Transforming Growth Factor-ß1 Arterioscler Thromb Vasc Biol, July 1, 1997; 17(7): 1251 - 1257. [Abstract] [Full Text]
|
|
|
|
|
|
S. d. F. Cortes, V. S. Lemos, and J.-C. Stoclet Alterations in Calcium Stores in Aortic Myocytes From Spontaneously Hypertensive Rats Hypertension, June 1, 1997; 29(6): 1322 - 1328. [Abstract] [Full Text]
|
|
|
|
|
|
R. S. Cunha, H. Dabire, I. Bezie, A. M. Weiss, K. Chaouche-Teyara, S. Laurent, M. E. Safar, and P. Lacolley Mechanical Stress of the Carotid Artery at the Early Phase of Spontaneous Hypertension in Rats Hypertension, April 1, 1997; 29(4): 992 - 998. [Abstract] [Full Text]
|
|
|
|
|
|
A. Sabri, B. I. Levy, P. Poitevin, L. Caputo, E. Faggin, F. Marotte, L. Rappaport, and J. L. Samuel Differential Roles of AT1 and AT2 Receptor Subtypes in Vascular Trophic and Phenotypic Changes in Response to Stimulation With Angiotensin II Arterioscler Thromb Vasc Biol, February 1, 1997; 17(2): 257 - 264. [Abstract] [Full Text]
|
|
|
|
|
|
P. Hamet Cancer and Hypertension: An Unresolved Issue Hypertension, September 1, 1996; 28(3): 321 - 324. [Full Text]
|
|
|
|
|
|
P. Hamet, L. Richard, T.-V. Dam, E. Teiger, S. N. Orlov, L. Gaboury, F. Gossard, and J. Tremblay Apoptosis in Target Organs of Hypertension Hypertension, October 1, 1995; 26(4): 642 - 648. [Abstract] [Full Text]
|
|
|
|
|
|
M. J.A.P. Daemen and J. G.R. De Mey Regional Heterogeneity of Arterial Structural Changes Hypertension, April 1, 1995; 25(4): 464 - 473. [Abstract] [Full Text]
|
|
|
|
|
|
E. Giasson and S. Meloche Role of p70 S6 Protein Kinase in Angiotensin II-induced Protein Synthesis in Vascular Smooth Muscle Cells J. Biol. Chem., March 10, 1995; 270(10): 5225 - 5231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Leduc and S. Meloche Angiotensin II Stimulates Tyrosine Phosphorylation of the Focal Adhesion-associated Protein Paxillin in Aortic Smooth Muscle Cells J. Biol. Chem., March 3, 1995; 270(9): 4401 - 4404. [Abstract] [Full Text] [PDF]
|
|