This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bolzon, B. J. Articles by Cheung, D. W. Search for Related Content PubMed PubMed Citation Articles by Bolzon, B. J. Articles by Cheung, D. W.

Hypertension, Vol 14, 137-144, Copyright © 1989 by American Heart Association

ARTICLES

Isolation and characterization of single vascular smooth muscle cells from spontaneously hypertensive rats

BJ Bolzon and DW Cheung
Department of Pharmacology, University of Toronto, Ontario, Canada.

To study the properties of vascular smooth muscle in hypertension without the influence of the nerves and endothelium, a procedure was developed to isolate single smooth muscle cells from tail arteries of spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) normotensive control rats. Perfusion of intact arteries with a solution of papain and collagenase produced dense populations of viable cells (more than 10(4) cells/ml) that remained relaxed in the presence of physiological levels of calcium. Contractile responses of smooth muscle cells from the SHR were significantly more sensitive to noradrenaline, potassium depolarization, and the calcium channel agonist Bay K 8644 compared with those from WKY rats. Enhanced sensitivity to calcium in the SHR was also observed on readdition of calcium to cells preincubated in noradrenaline or KCl in a calcium-free medium. These results provide evidence for alterations in the properties of vascular smooth muscle in the SHR at the single cell level.

This article has been cited by other articles:

				R. M. Touyz, G. He, L.-Y. Deng, and E. L. Schiffrin Role of Extracellular Signal-Regulated Kinases in Angiotensin II–Stimulated Contraction of Smooth Muscle Cells From Human Resistance Arteries Circulation, January 26, 1999; 99(3): 392 - 399. [Abstract] [Full Text] [PDF]

				R. M. Touyz and E. L. Schiffrin Angiotensin II Regulates Vascular Smooth Muscle Cell pH, Contraction, and Growth Via Tyrosine Kinase–Dependent Signaling Pathways Hypertension, August 1, 1997; 30(2): 222 - 229. [Abstract] [Full Text]

				I. M. Lozinskaya and R. H. Cox Effects of Age on Ca2+ Currents in Small Mesenteric Artery Myocytes From Wistar-Kyoto and Spontaneously Hypertensive Rats Hypertension, June 1, 1997; 29(6): 1329 - 1336. [Abstract] [Full Text]

				R. H. Cox and I. M. Lozinskaya Augmented Calcium Currents in Mesenteric Artery Branches of the Spontaneously Hypertensive Rat Hypertension, December 1, 1995; 26(6): 1060 - 1064. [Abstract] [Full Text]