Hypertension, Vol 18, 742-747, Copyright © 1991 by American Heart Association
P Delafontaine, H Lou and RW Alexander
We have previously demonstrated specific insulin-like growth factor I (IGF
I) messenger RNA (mRNA) transcripts in cultured rat aortic smooth muscle
cells (RASM). To define the role of IGF I in the autocrine growth program
of vascular smooth muscle cells, we quantitated IGF I mRNA levels in
proliferating and quiescent (serum-deprived for 48 hours) RASM. IGF I mRNA
levels were markedly decreased in quiescent cells, and this effect was
reversible on reexposure to serum. Since platelet-derived growth factor
(PDGF) acts synergistically with IGF I to stimulate vascular smooth muscle
cell growth, we exposed quiescent RASM to PDGF AB or BB and quantitated IGF
I transcript levels. Both PDGF dimers caused a marked, rapid increase in
IGF I message levels. To determine whether induction of IGF I mRNA levels
correlated with secretion of IGF I, we measured immunoreactive IGF I in
RASM conditioned medium after separation of IGF I binding proteins by gel
filtration chromatography. PDGF caused a significant increase in IGF I
release at 24 hours. These findings indicate that IGF I mRNA levels in
vitro are regulated by serum and by growth factors such as PDGF. Serum
deprivation reversibly decreases IGF I transcript levels, and exposure of
quiescent cells to PDGF increases IGF I mRNA levels and IGF I release.
Regulation of IGF I expression by competence growth factors such as PDGF
may play an important role in the control of vascular smooth muscle cell
growth.
ARTICLES
Regulation of insulin-like growth factor I messenger RNA levels in vascular smooth muscle cells
Department of Medicine, Emory University, Atlanta, GA.
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