Hypertension, Vol 20, 821-827, Copyright © 1992 by American Heart Association
K Skov, MJ Mulvany and N Korsgaard
We present a new perfusion technique that allows arteries down to the level
of capillaries to be fixed while relaxed and under a known intravascular
pressure. Through a catheter inserted into the right renal artery of
12-week-old male spontaneously hypertensive rats (n = 9) and control
Wistar-Kyoto rats (n = 11), the kidney vessels were rinsed with human
plasma, relaxed by papaverine, and perfused with a casting resin containing
microspheres. The microspheres (12 microns) became trapped in the glomeruli
of the kidney and, together with a closing of the venous outflow, they
caused the flow through the kidney to stop, so that the intravascular
pressure was raised to the level of the input perfusion pressure (100 mm
Hg). The resin material was allowed to harden, and the kidney was
immersion-fixed and prepared for histomorphometrical investigations. This
technique made it possible to measure both the structurally determined
lumen diameter and the corresponding media thickness under clearly defined
conditions. The lumen diameter of afferent arterioles close to the
glomeruli showed a 17% reduction in spontaneously hypertensive rats (15.4
+/- 0.6 microns; mean +/- SEM) compared with Wistar-Kyoto rat arterioles
(18.5 +/- 0.3 microns, p < 0.001). However, this was not due to media
hypertrophy, because media cross-sectional area was smaller (p < 0.001)
in spontaneously hypertensive rats (210 +/- 6 microns 2) compared with
Wistar-Kyoto rats (274 +/- 16 microns 2). We conclude that the lumen
reduction in renal afferent arterioles in spontaneously hypertensive rats
is not the result of an encroachment on the lumen by a hypertrophic media.
ARTICLES
Morphology of renal afferent arterioles in spontaneously hypertensive rats
Danish Biomembrane Research Centre, University of Aarhus, Denmark.
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