Hypertension, Vol 21, 827-835, Copyright © 1993 by American Heart Association
M Sibony, JM Gasc, F Soubrier, F Alhenc-Gelas and P Corvol
Angiotensin converting enzyme exists in two different isoforms, somatic and
germinal, whose respective distributions and intracellular localizations
have not been precisely determined. The differing biochemical and molecular
characteristics of the two isozymes allowed the preparation of antibodies
specific for each of the two angiotensin converting enzyme isoforms and of
two nucleic acid probes, one of which was specific for the germinal
isoform. Immunohistochemistry and in situ hybridization were used to
determine the cell distribution of, respectively, the two isoforms and
their corresponding messenger RNAs in the classically studied tissues of
male adult humans and marmosets. Results provided by the two different
methods were always concordant and were identical in the two species. The
somatic angiotensin converting enzyme form was expressed uniquely in
somatic tissues (vascular endothelial cells and at the brush border of
renal proximal convoluted tubule, jejunal villus, and epididymal duct
epithelia), and the germinal form was expressed uniquely in germinal cells
with a precise stage-specific pattern, starting in round spermatids and
finishing in spermatozoa. In situ hybridization documented the presence of
somatic angiotensin converting enzyme messenger RNA in renal tubule
epithelium, jejunal enterocytes, and epididymal epithelium and demonstrated
that there was no direct correlation between the levels of angiotensin
converting enzyme messenger RNA and the enzyme it encodes for, i.e.,
angiotensin converting enzyme, in a given epithelium. The significance of
the ultraselective expression of germinal angiotensin converting enzyme and
of its specific messenger RNA at a very precise stage of spermatogenesis
remains uncertain.
ARTICLES
Gene expression and tissue localization of the two isoforms of angiotensin I converting enzyme
INSERM U 36, Paris, France.
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|
|
|
|
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|
|
|
|
|
|
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|
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