This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by St Lezin, E. M. Articles by Morris, R. C. Search for Related Content PubMed PubMed Citation Articles by St Lezin, E. M. Articles by Morris, R. C., Jr

Hypertension, Vol 23, 786-790, Copyright © 1994 by American Heart Association

ARTICLES

Genetic contamination of Dahl SS/Jr rats. Impact on studies of salt- sensitive hypertension

EM St Lezin, M Pravenec, A Wong, JM Wang, T Merriouns, S Newton, DE Stec, RJ Roman, D Lau and RC Morris Jr
Department of Laboratory Medicine, University of California, San Francisco.

The Dahl salt-sensitive rat (SS/Jr) is a widely used animal model of salt-sensitive hypertension. SS/Jr rats are believed to be highly inbred and uniformly sensitive to the hypertensinogenic effects of sodium chloride, but we have recently observed that SS/Jr rats from Harlan Sprague Dawley, Inc, exhibit considerable variability in their blood pressure response to supplemental dietary salt. To test the possibility that commercially available SS/Jr rats are genetically contaminated and therefore no longer fully inbred, we performed molecular genetic studies and blood pressure measurements in several groups of SS/Jr rats purchased from Harlan Sprague Dawley. We found molecular evidence of heterozygosity and/or atypical allelic variants involving loci on at least five different chromosomes. Many of the rats also failed to exhibit a salt-sensitive blood pressure phenotype. We conclude that SS/Jr rats being sold by the only commercial vendor of Dahl rats in the United States are genetically contaminated and resistant to the hypertensinogenic effects of salt. These findings raise serious questions about the interpretation of research conducted with SS/Jr rats obtained from Harlan Sprague Dawley.

This article has been cited by other articles:

				J. P. Rapp Genetic Analysis of Inherited Hypertension in the Rat Physiol Rev, January 1, 2000; 80(1): 135 - 172. [Abstract] [Full Text] [PDF]

				E. St. Lezin, W. Liu, J.-M. Wang, Y. Yang, N. Qi, V. Kren, V. Zidek, T. W. Kurtz, and M. Pravenec Genetic Analysis of Rat Chromosome 1 and the Sa Gene in Spontaneous Hypertension Hypertension, January 1, 2000; 35(1): 225 - 230. [Abstract] [Full Text] [PDF]

				N. H. Garcia, C. F. Plato, B. A. Stoos, and J. L. Garvin Nitric Oxide–Induced Inhibition of Transport by Thick Ascending Limbs From Dahl Salt-Sensitive Rats Hypertension, September 1, 1999; 34(3): 508 - 513. [Abstract] [Full Text] [PDF]

				N. A. Mashburn, M. T. Unlap, J. Runquist, A. Alderman, G. V. W. Johnson, and P. D. Bell Altered protein kinase C activation of Na+/Ca2+ exchange in mesangial cells from salt-sensitive rats Am J Physiol Renal Physiol, April 1, 1999; 276(4): F574 - F580. [Abstract] [Full Text] [PDF]

				E. St. Lezin, L. Zhang, Y. Yang, J.-M. Wang, N. Wang, N. Qi, J. S. Steadman, W. Liu, V. Kren, V. Zidek, et al. Effect of Chromosome 19 Transfer on Blood Pressure in the Spontaneously Hypertensive Rat Hypertension, January 1, 1999; 33(1): 256 - 260. [Abstract] [Full Text] [PDF]

				P. Y. Chen, R. D. Gladish, and P. W. Sanders Vascular Smooth Muscle Nitric Oxide Synthase Anomalies in Dahl/Rapp Salt-Sensitive Rats Hypertension, April 1, 1998; 31(4): 918 - 924. [Abstract] [Full Text] [PDF]

				E. St. Lezin, W. Liu, N. Wang, J.-M. Wang, V. Kren, V. Zidek, M. Zdobinska, D. Krenova, A. Bottger, B. F. M. van Zutphen, et al. Effect of Renin Gene Transfer on Blood Pressure in the Spontaneously Hypertensive Rat Hypertension, January 1, 1998; 31(1): 373 - 377. [Abstract] [Full Text] [PDF]

				H. M. Kubisch, S. Mathialagan, and E. P. Gomez-Sanchez Modulation of Blood Pressure in the Dahl SS/jr Rat by Embryo Transfer Hypertension, January 1, 1998; 31(1): 540 - 545. [Abstract] [Full Text] [PDF]

				E. St. Lezin, W. Liu, J.-M. Wang, N. Wang, V. Kren, D. Krenova, A. Musilova, M. Zdobinska, V. Zidek, D. Lau, et al. Genetic Isolation of a Chromosome 1 Region Affecting Blood Pressure in the Spontaneously Hypertensive Rat Hypertension, October 1, 1997; 30(4): 854 - 858. [Abstract] [Full Text]

				J. Jiang, D. E. Stec, H. Drummond, J. S. Simon, G. Koike, H. J. Jacob, and R. J. Roman Transfer of a Salt-Resistant Renin Allele Raises Blood Pressure in Dahl Salt-Sensitive Rats Hypertension, February 1, 1997; 29(2): 619 - 627. [Abstract] [Full Text]

				W. G. Campbell Jr, F. Gahnem, D. F. Catanzaro, G. D. James, M. J. F. Camargo, J. H. Laragh, and J. E. Sealey Plasma and Renal Prorenin/Renin, Renin mRNA, and Blood Pressure in Dahl Salt-Sensitive and Salt-Resistant Rats Hypertension, May 1, 1996; 27(5): 1121 - 1133. [Abstract] [Full Text]

				R. Y. Walder, D. A. Morgan, W. G. Haynes, R. D. Sigmund, A. M. McClain, J. B. Stokes, and A. L. Mark Genetic Characterization of the `New' Harlan Sprague Dawley Dahl Salt-Sensitive Rats Hypertension, March 1, 1996; 27(3): 546 - 551. [Abstract] [Full Text]

				D. E. Stec, A. Y. Deng, J. P. Rapp, and R. J. Roman Cytochrome P4504A Genotype Cosegregates With Hypertension in Dahl S Rats Hypertension, March 1, 1996; 27(3): 564 - 568. [Abstract] [Full Text]

				A.-P. Zou, H. A. Drummond, and R. J. Roman Role of 20-HETE in Elevating Loop Chloride Reabsorption in Dahl SS/Jr Rats Hypertension, March 1, 1996; 27(3): 631 - 635. [Abstract] [Full Text]

				C. M. Cover, J.-M. Wang, E. St. Lezin, T. W. Kurtz, and S. H. Mellon Molecular Variants in the P450c11AS Gene as Determinants of Aldosterone Synthase Activity in the Dahl Rat Model of Hypertension J. Biol. Chem., July 14, 1995; 270(28): 16555 - 16560. [Abstract] [Full Text] [PDF]

				J.-Q. Kong, D. A. Taylor, and W. W. Fleming Sustained Hypertension in Dahl Rats : Negative Correlation of Agonist Response to Blood Pressure Hypertension, January 1, 1995; 25(1): 139 - 145. [Abstract] [Full Text]