Hypertension, Vol 24, 77-82, Copyright © 1994 by American Heart Association
RD McCabe, MA Bakarich, K Srivastava and DB Young
We conducted this study to determine whether physiological changes in
potassium concentration affect free radical formation by vascular cells. We
assessed the effects of potassium on reactive oxygen species formed by
cultured endothelial and monocyte/macrophage cells or freshly isolated
human white blood cells by cytochrome c reduction or luminol
chemiluminescence, respectively. Reducing potassium concentration of
endothelial cell media (normally 5.1 to 6.1 mmol/L) to 3.0 mmol/L
exponentially increased the rate of cytochrome c reduction, up to 8.4- fold
at 2 hours; raising potassium concentration to 5.5 or 7.0 mmol/L at 1 hour
reduced the maximal rate of cytochrome c reduction by 86% or 93%.
Subsequent studies were done 30 to 75 minutes after media change. Potassium
reduced the rate of cytochrome c reduction by 49% (endothelial cells) to
55% (monocytes/macrophages) between 3.0 and 7.0 mmol/L; the greatest
decrement (20% to 26%) occurred between 3.0 and 4.0 mmol/L. Superoxide
dismutase reduced the rate of cytochrome c reduction by 62% or 50% in
endothelial or monocyte/macrophage cells. Potassium had no effect on the
rate of cytochrome c reduction in the presence of superoxide dismutase.
Increasing potassium concentration from 1.48 to 4.77 or 7.94 mmol/L also
reduced luminol chemiluminescence in human white blood cells challenged by
1 to 10 mg/mL zymosan. We conclude that physiological increases in
potassium concentration inhibit the rate of superoxide anion formation by
cell lines derived from endothelium and from monocytes/macrophages and
reactive oxygen species formation by human white blood cells.
ARTICLES
Potassium inhibits free radical formation
Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson 39216-4505.
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