Hypertension, Vol 24, 734-738, Copyright © 1994 by American Heart Association
PA Lucchesi, N DeRoux and BC Berk
The Na(+)-H+ exchanger has important modulatory effects on vascular smooth
muscle cell proliferation and contractility. Increased Na(+)-H+ exchange
activity is a general property of many tissues, including mesenteric artery
and cultured vascular smooth muscle cells, in the spontaneously
hypertensive rat (SHR). In the present work, we investigated whether
alterations in the steady-state levels of specific Na(+)-H+ exchanger mRNA
isoforms (NHE-1 through NHE-4) are associated with the observed increases
in exchanger activity. Poly(A+) mRNA prepared from 12-week-old hypertensive
SHR and normotensive Wistar- Kyoto (WKY) aorta, kidney, and intestine was
hybridized to cDNAs specific for each NHE isoform. By Northern blot
analysis, NHE-1 was detected in all tissues as well as cultured vascular
smooth muscle cells and was not regulated differently in SHR compared with
WKY tissues. There was no expression of NHE-2, NHE-3, or NHE-4 in SHR and
WKY aortas or in cultured vascular smooth muscle cells from SHR and WKY
aortas. Stimulation of NHE-1 mRNA expression by growth factors was similar
in cultured SHR and WKY vascular smooth muscle cells. We conclude that the
previously observed increase in exchanger activity in blood vessels and
cultured vascular smooth muscle cells of the SHR is not caused by induction
of the NHE-2, NHE-3, and NHE-4 isoforms or by alterations in steady-state
NHE-1 mRNA expression. These findings suggest that posttranslational
regulation of the Na(+)-H+ exchanger is responsible for increased activity
in the SHR.
ARTICLES
Na(+)-H+ exchanger expression in vascular smooth muscle of spontaneously hypertensive and Wistar-Kyoto rats
Department of Medicine, Emory University School of Medicine, Atlanta, Ga.
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