(Hypertension. 1995;25:449-452.)
© 1995 American Heart Association, Inc.
Articles |
Pressure Enhances Endothelin-1 Release From Cultured Human Endothelial Cells
From the Departments of Internal Medicine and Pharmacology (T.N., R.K.), Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan.
Correspondence to Toshio Nakaki, MD, PhD, Department of Pharmacology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160 Japan.
Abstract The effect of pure pressure without shear stress or stretch on the release of endothelin-1 was investigated. Elevation of pressure significantly enhanced endothelin-1 release from cultured human umbilical vein endothelial cells. A calcium channel blocker, nifedipine, and a putative stretch-activated channel blocker, gadolinium, did not affect the pressure-induced endothelin-1 increase. On the other hand, a phospholipase C inhibitor, 2-nitro-4-carboxyphenyl-N,N-diphenylcarbamate, and protein kinase C inhibitors, 1-5-(isoquinolinylsulfonyl)-2-methylpiperazine and chelerythrine, significantly inhibited the pressure-induced endothelin-1 increase. Moreover, pure pressure reduced basal nitric oxide release, while pretreatment with a nitric oxide synthase inhibitor, NG-monomethyl-L-arginine, had no effect on the pressure-induced endothelin-1 increase. In conclusion, our results show for the first time that pressure enhances endothelin-1 release partially through activation of phospholipase C and protein kinase C in human endothelial cells.
Key Words: endothelin-1 • mechanoreception • Ca2+ channel • stretch • pressure • nitric oxide
This article has been cited by other articles:
 |
|
 |
|
  J. L. Garvin and N. J. Hong Cellular Stretch Increases Superoxide Production in the Thick Ascending Limb Hypertension, February 1, 2008; 51(2): 488 - 493. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. A. Lee, E. B. Baek, K. S. Park, H. J. Jung, J. I. Kim, S. J. Kim, and Y. E Earm Mechanosensitive nonselective cation channel facilitation by endothelin-1 is regulated by protein kinase C in arterial myocytes Cardiovasc Res, November 1, 2007; 76(2): 224 - 235. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. P. Conrad Mechanisms of Renal Vasodilation and Hyperfiltration During Pregnancy Reproductive Sciences, October 1, 2004; 11(7): 438 - 448. [Abstract] [PDF]
|
|
|
|
 |
|
 K. P. Conrad and J. Novak Emerging role of relaxin in renal and cardiovascular function Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2004; 287(2): R250 - R261. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. B. C. Coumans, Y. Garnier, S. Supcun, A. Jense, R. Berger, and T. H. M. Hasaart The Effects of Low-Dose Endotoxin on the Umbilicoplacental Circulation in Preterm Sheep Reproductive Sciences, July 1, 2004; 11(5): 289 - 293. [Abstract] [PDF]
|
|
|
|
|
|
Y. Garnier, A. B. C. Coumans, A. Jensen, T. H. M. Hasaart, and R. Berger Infection-Related Perinatal Brain Injury: The Pathogenic Role of Impaired Fetal Cardiovascular Control Reproductive Sciences, December 1, 2003; 10(8): 450 - 459. [Abstract] [PDF]
|
|
|
|
 |
|
 K. Minamoto, D. J. Pinsky, T. Fujita, and Y. Naka Timing of Nitric Oxide Donor Supplementation Determines Endothelin-1 Regulation and Quality of Lung Preservation for Transplantation Am. J. Respir. Cell Mol. Biol., January 1, 2002; 26(1): 14 - 21. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Dumont, M. D'Amours, M. Lebel, and R. Lariviere Supplementation with a low dose of l-arginine reduces blood pressure and endothelin-1 production in hypertensive uraemic rats Nephrol. Dial. Transplant., April 1, 2001; 16(4): 746 - 754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-m. Gan, L. Selin-Sjogren, R. Doroudi, and S. Jern Temporal regulation of endothelial ET-1 and eNOS expression in intact human conduit vessels exposed to different intraluminal pressure levels at physiological shear stress Cardiovasc Res, October 1, 2000; 48(1): 168 - 177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Kusaka, R. A. Kelly, G. H. Williams, and I. Kifor Coronary microvascular endothelial cells cosecrete angiotensin II and endothelin-1 via a regulated pathway Am J Physiol Heart Circ Physiol, September 1, 2000; 279(3): H1087 - H1096. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. S. Wittstein, W. Qiu, R. C. Ziegelstein, Q. Hu, and D. A. Kass Opposite Effects of Pressurized Steady Versus Pulsatile Perfusion on Vascular Endothelial Cell Cytosolic pH : Role of Tyrosine Kinase and Mitogen-Activated Protein Kinase Signaling Circ. Res., June 23, 2000; 86(12): 1230 - 1236. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Malek, S. L. Alper, and S. Izumo Hemodynamic Shear Stress and Its Role in Atherosclerosis JAMA, December 1, 1999; 282(21): 2035 - 2042. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Xie, J. A. Bevan, and W. G. Mayhan Oxidized Low-Density Lipoprotein Enhances Myogenic Tone in the Rabbit Posterior Cerebral Artery Through the Release of Endothelin-1 • Editorial Comment Stroke, November 1, 1999; 30 (11): 2423 - 2430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Schiffrin Role of Endothelin-1 in Hypertension Hypertension, October 1, 1999; 34(4): 876 - 881. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Henrion, M. Iglarz, and B. I. Levy Chronic Endothelin-1 Improves Nitric Oxide–Dependent Flow-Induced Dilation in Resistance Arteries From Normotensive and Hypertensive Rats Arterioscler. Thromb. Vasc. Biol., September 1, 1999; 19(9): 2148 - 2153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Brochu, S. Lacasse-M., C. Moreau, M. Lebel, I. Kingma, J. H. Grose, and R. Lariviere Endothelin ETA receptor blockade prevents the progression of renal failure and hypertension in uraemic rats Nephrol. Dial. Transplant., August 1, 1999; 14(8): 1881 - 1888. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Tarbell, L. Demaio, and M. M. Zaw Effect of pressure on hydraulic conductivity of endothelial monolayers: role of endothelial cleft shear stress J Appl Physiol, July 1, 1999; 87(1): 261 - 268. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Marano, M. Grigioni, S. Palazzesi, and A. U Ferrari Endothelin and mechanical properties of the carotid artery in Wistar-Kyoto and spontaneously hypertensive rats Cardiovasc Res, March 1, 1999; 41(3): 701 - 707. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Iglarz, K. Matrougui, B. I. Levy, and D. Henrion Chronic blockade of endothelin ETA receptors improves flow dependent dilation in resistance arteries of hypertensive rats Cardiovasc Res, September 1, 1998; 39(3): 657 - 664. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Caldwell, H. F. Clemo, and C. M. Baumgarten Using gadolinium to identify stretch-activated channels: technical considerations Am J Physiol Cell Physiol, August 1, 1998; 275(2): C619 - C621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zanchi, N. Stergiopulos, H. R. Brunner, and D. Hayoz Differences in the Mechanical Properties of the Rat Carotid Artery In Vivo, In Situ, and In Vitro Hypertension, July 1, 1998; 32(1): 180 - 185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ziegler, K. Bouzourene, V. J. Harrison, H. R. Brunner, and D. Hayoz Influence of Oscillatory and Unidirectional Flow Environments on the Expression of Endothelin and Nitric Oxide Synthase in Cultured Endothelial Cells Arterioscler. Thromb. Vasc. Biol., May 1, 1998; 18(5): 686 - 692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hishikawa, B. S. Oemar, Z. Yang, and T. F. Luscher Pulsatile Stretch Stimulates Superoxide Production and Activates Nuclear Factor-{kappa}B in Human Coronary Smooth Muscle Circ. Res., November 19, 1997; 81(5): 797 - 803. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. Hishikawa and T. F. Luscher Pulsatile Stretch Stimulates Superoxide Production in Human Aortic Endothelial Cells Circulation, November 18, 1997; 96(10): 3610 - 3616. [Abstract] [Full Text]
|
|
|
|
|
|
R. Largo, D. Gomez-Garre, X. H. Liu, J. Alonso, J. Blanco, J. J. Plaza, and J. Egido Endothelin-1 Upregulation in the Kidney of Uninephrectomized Spontaneously Hypertensive Rats and Its Modification by the Angiotensin-Converting Enzyme Inhibitor Quinapril Hypertension, May 1, 1997; 29(5): 1178 - 1185. [Abstract] [Full Text]
|
|
|
|
|
|
T. Tonnessen, G. Christensen, E. Oie, E. Holt, H. Kjekshus, O. A. Smiseth, O. M. Sejersted, and H. Attramadal Increased cardiac expression of endothelin-1 mRNA in ischemic heart failure in rats Cardiovasc Res, March 1, 1997; 33(3): 601 - 610. [Abstract] [PDF]
|
|
|
|
|
|
D. Hasdai, D. R. Holmes, K. N. Garratt, W. D. Edwards, and A. Lerman Mechanical Pressure and Stretch Release Endothelin-1 From Human Atherosclerotic Coronary Arteries In Vivo Circulation, January 21, 1997; 95(2): 357 - 362. [Abstract] [Full Text]
|
|
|
|
|
|
D. Dunbar Ivy, J. P. Kinsella, and S. H. Abman Endothelin blockade augments pulmonary vasodilation in the ovine fetus J Appl Physiol, December 1, 1996; 81(6): 2481 - 2487. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. R. Knoblich, R. H. Freeman, and D. Villarreal Pressure-Dependent Renin Release During Chronic Blockade of Nitric Oxide Synthase Hypertension, November 1, 1996; 28(5): 738 - 742. [Abstract] [Full Text]
|
|
|
|
|
|
P. Sventek, J.-S. Li, K. Grove, C. F. Deschepper, and E. L. Schiffrin Vascular Structure and Expression of Endothelin-1 Gene in L-NAME–Treated Spontaneously Hypertensive Rats Hypertension, January 1, 1996; 27(1): 49 - 55. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. Hishikawa, B. S. Oemar, and T. Nakaki Static Pressure Regulates Connective Tissue Growth Factor Expression in Human Mesangial Cells J. Biol. Chem., May 11, 2001; 276(20): 16797 - 16803. [Abstract] [Full Text] [PDF]
|
|