(Hypertension. 1995;25:1111-1115.)
© 1995 American Heart Association, Inc.
Articles |
A Noninvasive Computerized Tail-Cuff System for Measuring Blood Pressure in Mice
From the Departments of Pathology and Medicine, University of North Carolina at Chapel Hill.
Correspondence to Dr John H. Krege, 703 B.B.B., CB #7525, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599. E-mail krege@med.unc.edu.
Abstract We have validated a noninvasive computerized tail-cuff system for measuring blood pressure in mice. The system was designed to perform all functions automatically, including a programmable routine of cuff inflation and deflation, analysis and assignment of pulse rate and blood pressure, and recording of data electronically. To evaluate this system over a range of blood pressures, we gave groups of mice enalapril or NG-nitro-L-arginine methyl ester in their drinking water. For each of these groups, an equal number of control mice were given nothing in their drinking water. Tail-cuff blood pressures were recorded as the means of blood pressures determined on at least 3 days after at least 7 days of training. Tail-cuff enalapril and control group means were measured both 3 and 4 months after enalapril (or no drug) was begun; the group means at 3 months were not significantly different from the group means at 4 months. These results demonstrate that the system gives reproducible results. After the tail-cuff measurements were completed, intra-arterial blood pressures were attempted in all mice under unrestrained, unanesthetized conditions, and individual mouse (n=22) blood pressures with the use of the two methods were compared. The blood pressures from individual mice by tail-cuff and intra-arterial methods were highly correlated (r=.86, P<.01). The means for the four mouse groups were also highly correlated (r=.98, P<.02). These data show that blood pressures measured on trained mice by a computerized noninvasive tail-cuff system are reproducible and correlate well with intra-arterial blood pressures measured on unrestrained, unanesthetized mice.
Key Words: blood pressure determination, noninvasive • genetics • mice
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