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(Hypertension. 1995;26:177-185.)
© 1995 American Heart Association, Inc.

Articles

Endothelial Stimulation of Sodium Pump in Cultured Vascular Smooth Muscle

Juliana Redondo; Concepción Peiró; Leocadio Rodríguez-Mañas; Mercedes Salaices; Jesús Marín; Carlos F. Sánchez-Ferrer

From the Departamento de Farmacología y Terapéutica, Facultad de Medicina, Universidad Autónoma, and Unidad de Investigación y Servicio de Geriatría, Hospital Universitario de Getafe (L.R.-M.), Madrid, Spain.

Abstract We studied vascular sodium pump activity and its regulation by vasoactive agents and endothelium in cultured aortic vascular smooth muscle cells from normotensive Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR). Baseline sodium pump activity (ouabain-inhibitable ⁸⁶Rb⁺ uptake) was similar in cells from both rat strains. Angiotensin II and endothelin-1 increased ouabain-inhibitable ⁸⁶Rb⁺ uptake more in SHR than WKY cells, whereas no effects were obtained with sodium nitroprusside, 8-bromo-cGMP, or iloprost. We examined the influence of endothelium on vascular sodium pump activity either by coculturing smooth muscle and endothelial cells or by using conditioned medium. Both coculture for 24 hours with endothelial cells and treatment with conditioned medium increased smooth muscle cell sodium pump activity, this effect being higher in SHR cells. These results suggest that the endothelium may modulate sodium pump activity in the underlying smooth muscle by releasing a diffusible compound, which is more active on SHR smooth muscle. The conditioned medium obtained in the presence of inhibitors of angiotensin-converting enzyme, endothelin-1–converting enzyme, cyclooxygenase, lipoxygenase, and nitric oxide synthase had no effect on the ability of conditioned medium to increase sodium pump activity, suggesting that angiotensin II, endothelin-1, eicosanoids, and nitric oxide are not involved in this stimulatory effect. The nature of the possible endothelial factor involved is still unknown, but it possesses a molecular weight between 25 and 50 kD, is heat stable, and is sensitive to trypsin treatment. We propose it could be a growth factor.

Key Words: endothelium • muscle, smooth, vascular • Na⁺-K⁺–exchanging ATPase • ouabain • hypertension, sodium-dependent

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