| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
(Hypertension. 1995;26:642-648.)
© 1995 American Heart Association, Inc.
Articles |
Apoptosis in Target Organs of Hypertension
Presented in part at the 14th International Society of Hypertension Congress, Melbourne, Australia, March 1994.
From the Centre de Recherche Hôtel-Dieu de Montréal, Université de Montréal (Canada).
Correspondence to Pavel Hamet, Laboratory of Molecular Pathophysiology, Centre de Recherche Hôtel-Dieu de Montréal, 3850 St. Urbain St, Montréal, Québec H2W 1T8, Canada.
Abstract Apoptosis or programmed cell death frequently parallels abnormalities in cell proliferation and differentiation. As hypertrophy/hyperplasia or remodeling occurs in organs affected by hypertension, we evaluated the degree of apoptosis in the heart, kidney, and brain in situ in genetically hypertensive mice and rats as well as in cultured vascular smooth muscle cells. Apoptosis was characterized by morphological features, DNA fragmentation, and laddering as well as by terminal deoxynucleotidyl transferase labeling of the 3' OH ends of both extracted DNA and tissue sections. The present report provides the first evidence of increased apoptosis in whole organs of genetically hypertensive rat and mouse strains: in the heart of spontaneously hypertensive rats (SHR) and in the heart (ventricular cardiomyocytes), kidney (inner cortex and medulla), and brain (cortex, striatum, hippocampus, and thalamus) of spontaneously hypertensive mice, with a higher effect of apoptotic inducers in cultured aortic smooth muscle cells derived from SHR. Both types of known apoptotic processes, oligonucleosomal cleavage and large DNA fragmentation, were observed in vascular smooth muscle cells, but only the former appeared to be increased in SHR. This study underlines the importance of cell death dysregulation in hypertension, reveals a new route for investigation of the pathogenesis of hypertension, and suggests novel targets of therapeutic intervention.
Key Words: apoptosis • hypertension • muscle, smooth, vascular • heart • kidney
This article has been cited by other articles:
 |
|
 |
|
  K. Kitahori, H. He, M. Kawata, D. B. Cowan, I. Friehs, P. J. del Nido, and F. X. McGowan Jr Development of Left Ventricular Diastolic Dysfunction With Preservation of Ejection Fraction During Progression of Infant Right Ventricular Hypertrophy Circ Heart Fail, November 1, 2009; 2(6): 599 - 607. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. Garciarena, C. I. Caldiz, E. L. Portiansky, G. E. Chiappe de Cingolani, and I. L. Ennis Chronic NHE-1 blockade induces an antiapoptotic effect in the hypertrophied heart J Appl Physiol, April 1, 2009; 106(4): 1325 - 1331. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. Garciarena, O. A. Pinilla, M. B. Nolly, R. P. Laguens, E. M. Escudero, H. E. Cingolani, and I. L. Ennis Endurance Training in the Spontaneously Hypertensive Rat: Conversion of Pathological into Physiological Cardiac Hypertrophy Hypertension, April 1, 2009; 53(4): 708 - 714. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Sharma, S. Dhingra, N. Khaper, and P. K. Singal Activation of apoptotic processes during transition from hypertrophy to heart failure in guinea pigs Am J Physiol Heart Circ Physiol, September 1, 2007; 293(3): H1384 - H1390. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Okubo, Y. Tanabe, K. Takeda, M. Kitayama, S. Kanemitsu, R. C. Kukreja, and N. Takekoshi Ischemic preconditioning and morphine attenuate myocardial apoptosis and infarction after ischemia-reperfusion in rabbits: role of {delta}-opioid receptor Am J Physiol Heart Circ Physiol, October 1, 2004; 287(4): H1786 - H1791. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Gonzalez, M. A Fortuno, R. Querejeta, S. Ravassa, B. Lopez, N. Lopez, and J. Diez Cardiomyocyte apoptosis in hypertensive cardiomyopathy Cardiovasc Res, September 1, 2003; 59(3): 549 - 562. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-J. Gao, H. Yang, K. Teoh, and R. M.K.W. Lee Detrimental effects of papaverine on the human internal thoracic artery J. Thorac. Cardiovasc. Surg., July 1, 2003; 126(1): 179 - 185. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Ragolia, T. Palaia, E. Paric, and J. K. Maesaka Prostaglandin D2 Synthase Inhibits the Exaggerated Growth Phenotype of Spontaneously Hypertensive Rat Vascular Smooth Muscle Cells J. Biol. Chem., June 6, 2003; 278(24): 22175 - 22181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Fortuno, A. Gonzalez, S. Ravassa, B. Lopez, and J. Diez Clinical implications of apoptosis in hypertensive heart disease Am J Physiol Heart Circ Physiol, May 1, 2003; 284(5): H1495 - H1506. [Full Text] [PDF]
|
|
|
|
 |
|
 S. Taurin, V. Seyrantepe, S. N. Orlov, T.-L. Tremblay, P. Thibault, M. R. Bennett, P. Hamet, and A. V. Pshezhetsky Proteome Analysis and Functional Expression Identify Mortalin as an Antiapoptotic Gene Induced by Elevation of [Na+]i/[K+]i Ratio in Cultured Vascular Smooth Muscle Cells Circ. Res., November 15, 2002; 91(10): 915 - 922. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sotoudeh, Y.-S. Li, N. Yajima, C.-C. Chang, T.-C. Tsou, Y. Wang, S. Usami, A. Ratcliffe, S. Chien, and J. Y.-J. Shyy Induction of apoptosis in vascular smooth muscle cells by mechanical stretch Am J Physiol Heart Circ Physiol, May 1, 2002; 282(5): H1709 - H1716. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gonzalez, B. Lopez, S. Ravassa, R. Querejeta, M. Larman, J. Diez, and M. A. Fortuno Stimulation of Cardiac Apoptosis in Essential Hypertension: Potential Role of Angiotensin II Hypertension, January 1, 2002; 39(1): 75 - 80. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Fortuno, S. Ravassa, A. Fortuno, G. Zalba, and J. Diez Cardiomyocyte Apoptotic Cell Death in Arterial Hypertension: Mechanisms and Potential Management Hypertension, December 1, 2001; 38(6): 1406 - 1412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. D. Intengan and E. L. Schiffrin Vascular Remodeling in Hypertension: Roles of Apoptosis, Inflammation, and Fibrosis Hypertension, September 1, 2001; 38(3): 581 - 587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Hamet, N. Thorin-Trescases, P. Moreau, P. Dumas, B.-S. Tea, D. deBlois, V. Kren, M. Pravenec, J. Kunes, Y. Sun, et al. Workshop: Excess Growth and Apoptosis : Is Hypertension a Case of Accelerated Aging of Cardiovascular Cells? Hypertension, February 1, 2001; 37(2): 760 - 766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ravassa, M. A. Fortuno, A. Gonzalez, B. Lopez, G. Zalba, A. Fortuno, and J. Diez Mechanisms of Increased Susceptibility to Angiotensin II-Induced Apoptosis in Ventricular Cardiomyocytes of Spontaneously Hypertensive Rats Hypertension, December 1, 2000; 36(6): 1065 - 1071. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. H. Lorell and B. A. Carabello Left Ventricular Hypertrophy : Pathogenesis, Detection, and Prognosis Circulation, July 25, 2000; 102(4): 470 - 479. [Full Text] [PDF]
|
|
|
|
|
|
A. M. Devlin, J. S. Clark, J. L. Reid, and A. F. Dominiczak DNA Synthesis and Apoptosis in Smooth Muscle Cells From a Model of Genetic Hypertension Hypertension, July 1, 2000; 36(1): 110 - 115. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. N. Orlov, S. Taurin, N. Thorin-Trescases, N. O. Dulin, J. Tremblay, and P. Hamet Inversion of the Intracellular Na+/K+ Ratio Blocks Apoptosis in Vascular Smooth Muscle Cells by Induction of RNA Synthesis Hypertension, May 1, 2000; 35(5): 1062 - 1068. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-S. Tea, S. Der Sarkissian, R. M. Touyz, P. Hamet, and D. deBlois Proapoptotic and Growth-Inhibitory Role of Angiotensin II Type 2 Receptor in Vascular Smooth Muscle Cells of Spontaneously Hypertensive Rats In Vivo Hypertension, May 1, 2000; 35(5): 1069 - 1073. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Schumann, J. Holtz, H.-R. Zerkowski, and M. Hatzfeld Expression of secreted frizzled related proteins 3 and 4 in human ventricular myocardium correlates with apoptosis related gene expression Cardiovasc Res, February 1, 2000; 45(3): 720 - 728. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. McCarthy and M. Bennett The regulation of vascular smooth muscle cell apoptosis Cardiovasc Res, February 1, 2000; 45(3): 747 - 755. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. P. Mason Calcium channel blockers, apoptosis and cancer: is there a biologic relationship? J. Am. Coll. Cardiol., December 1, 1999; 34(7): 1857 - 1866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ono, H. Ono, H. Matsuoka, T. Fujimori, and E. D. Frohlich Apoptosis, Coronary Arterial Remodeling, and Myocardial Infarction After Nitric Oxide Inhibition in SHR Hypertension, October 1, 1999; 34(4): 609 - 616. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-S. Tea, T.-V. Dam, P. Moreau, P. Hamet, and D. deBlois Apoptosis During Regression of Cardiac Hypertrophy in Spontaneously Hypertensive Rats : Temporal Regulation and Spatial Heterogeneity Hypertension, August 1, 1999; 34(2): 229 - 235. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Jun Zhang, C. G. Duarte, and S. Ellis Contrast Medium- and Mannitol-Induced Apoptosis in Heart and Kidney of SHR Rats Toxicol Pathol, July 1, 1999; 27(4): 427 - 435. [Abstract] [PDF]
|
|
|
|
|
|
S. Assadnia, J. P. Rapp, A. L. Nestor, T. Pringle, G. J. Cerilli, W. T. Gunning III, T. H. Webb, M. Kligman, and D. C. Allison Strain Differences in Neointimal Hyperplasia in the Rat Circ. Res., June 11, 1999; 84(11): 1252 - 1257. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. N. Orlov, N. Thorin-Trescases, S. V. Kotelevtsev, J. Tremblay, and P. Hamet Inversion of the Intracellular Na+/K+ Ratio Blocks Apoptosis in Vascular Smooth Muscle at a Site Upstream of Caspase-3 J. Biol. Chem., June 4, 1999; 274(23): 16545 - 16552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Suzuki, F. A. Delano, N. Jamshidi, D. Katz, M. Mori, K. Kosaki, R. A. Gottlieb, H. Ishii, and G. W. Schmid-Schonbein Enhanced DNA fragmentation in the thymus of spontaneously hypertensive rats Am J Physiol Heart Circ Physiol, June 1, 1999; 276(6): H2135 - H2140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Fortuno, G. Zalba, S. Ravassa, E. D'Elom, F. J. Beaumont, A. Fortuno, and J. Diez p53-Mediated Upregulation of BAX Gene Transcription Is Not Involved in Bax-{alpha} Protein Overexpression in the Left Ventricle of Spontaneously Hypertensive Rats Hypertension, June 1, 1999; 33(6): 1348 - 1352. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. N. Orlov, N. C. Adragna, V. A. Adarichev, and P. Hamet Genetic and biochemical determinants of abnormal monovalent ion transport in primary hypertension Am J Physiol Cell Physiol, March 1, 1999; 276(3): C511 - C536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-J. Champagne, P. Dumas, S. N. Orlov, M. R. Bennett, P. Hamet, and J. Tremblay Protection Against Necrosis but Not Apoptosis by Heat-Stress Proteins in Vascular Smooth Muscle Cells : Evidence for Distinct Modes of Cell Death Hypertension, March 1, 1999; 33(3): 906 - 913. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. M. Best, D. Hasdai, G. Sangiorgi, R. S. Schwartz, D. R. Holmes Jr, R. D. Simari, and A. Lerman Apoptosis : Basic Concepts and Implications in Coronary Artery Disease Arterioscler Thromb Vasc Biol, January 1, 1999; 19(1): 14 - 22. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Fortuno, S. Ravassa, J. C. Etayo, and J. Diez Overexpression of Bax Protein and Enhanced Apoptosis in the Left Ventricle of Spontaneously Hypertensive Rats : Effects of AT1 Blockade With Losartan Hypertension, August 1, 1998; 32(2): 280 - 286. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A Doevendans, M. J. Daemen, E. D de Muinck, and J. F Smits Cardiovascular phenotyping in mice Cardiovasc Res, July 1, 1998; 39(1): 34 - 49. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A Doggrell and L. Brown Rat models of hypertension, cardiac hypertrophy and failure Cardiovasc Res, July 1, 1998; 39(1): 89 - 105. [Full Text] [PDF]
|
|
|
|
|
|
T. Shioi, A. Matsumori, Y. Kihara, M. Inoko, K. Ono, Y. Iwanaga, T. Yamada, A. Iwasaki, K. Matsushima, and S. Sasayama Increased Expression of Interleukin-1ß and Monocyte Chemotactic and Activating Factor/Monocyte Chemoattractant Protein-1 in the Hypertrophied and Failing Heart With Pressure Overload Circ. Res., November 19, 1997; 81(5): 664 - 671. [Abstract] [Full Text]
|
|
|
|
|
|
P.-F. Li, R. Dietz, and R. von Harsdorf Differential Effect of Hydrogen Peroxide and Superoxide Anion on Apoptosis and Proliferation of Vascular Smooth Muscle Cells Circulation, November 18, 1997; 96(10): 3602 - 3609. [Abstract] [Full Text]
|
|
|
|
|
|
J. Diez, A. Panizo, M. Hernandez, F. Vega, I. Sola, M. A. Fortuno, and J. Pardo Cardiomyocyte Apoptosis and Cardiac Angiotensin-Converting Enzyme in Spontaneously Hypertensive Rats Hypertension, November 1, 1997; 30(5): 1029 - 1034. [Abstract] [Full Text]
|
|
|
|
|
|
M. Shichiri, H. Kato, F. Marumo, and Y. Hirata Endothelin-1 as an Autocrine/Paracrine Apoptosis Survival Factor for Endothelial Cells Hypertension, November 1, 1997; 30(5): 1198 - 1203. [Abstract] [Full Text]
|
|
|
|
|
|
P. Moreau, B.-S. Tea, T.-V. Dam, and P. Hamet Altered Balance Between Cell Replication and Apoptosis in Hearts and Kidneys of Newborn SHR Hypertension, September 1, 1997; 30(3): 720 - 724. [Abstract] [Full Text]
|
|
|
|
|
|
W. R. MacLellan and M. D. Schneider Death by Design : Programmed Cell Death in Cardiovascular Biology and Disease Circ. Res., August 19, 1997; 81(2): 137 - 144. [Abstract] [Full Text]
|
|
|
|
|
|
A. Aviv and H. Aviv Reflections on Telomeres, Growth, Aging, and Essential Hypertension Hypertension, May 1, 1997; 29(5): 1067 - 1072. [Abstract] [Full Text]
|
|
|
|
|
|
J. Diez, A. Panizo, M. Hernandez, and J. Pardo Is the Regulation of Apoptosis Altered in Smooth Muscle Cells of Adult Spontaneously Hypertensive Rats? Hypertension, March 1, 1997; 29(3): 776 - 780. [Abstract] [Full Text]
|
|
|
|
|
|
D. deBlois, B.-S. Tea, T.-V. Dam, J. Tremblay, and P. Hamet Smooth Muscle Apoptosis During Vascular Regression in Spontaneously Hypertensive Rats Hypertension, January 1, 1997; 29(1): 340 - 344. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Fliss and D. Gattinger Apoptosis in Ischemic and Reperfused Rat Myocardium Circ. Res., November 1, 1996; 79(5): 949 - 956. [Abstract] [Full Text]
|
|
|
|
 |
|
 W. S. Colucci Apoptosis in the Heart N. Engl. J. Med., October 17, 1996; 335(16): 1224 - 1226. [Full Text]
|
|
|
|
|
|
D. deBlois, M. Viswanathan, J.E. Su, A.W. Clowes, J.M. Saavedra, and S.M. Schwartz Smooth Muscle DNA Replication in Response to Angiotensin II Is Regulated Differently in the Neointima and Media at Different Times After Balloon Injury in the Rat Carotid Artery: Role of AT1 Receptor Expression Arterioscler Thromb Vasc Biol, September 1, 1996; 16(9): 1130 - 1137. [Abstract] [Full Text]
|
|
|
|
|
|
P. Hamet Cancer and Hypertension: An Unresolved Issue Hypertension, September 1, 1996; 28(3): 321 - 324. [Full Text]
|
|
|
|
|
|
Y. Nakamura, R. Morishita, S. Nakamura, M. Aoki, A. Moriguchi, K. Matsumoto, T. Nakamura, J. Higaki, and T. Ogihara A Vascular Modulator, Hepatocyte Growth Factor, Is Associated With Systolic Pressure Hypertension, September 1, 1996; 28(3): 409 - 413. [Abstract] [Full Text]
|
|