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(Hypertension. 1995;26:733.)
© 1995 American Heart Association, Inc.

Articles

Renin and Angiotensin II Receptor Gene Expression in Kidneys of Renal Hypertensive Rats

Jacques-Antoine Haefliger¹; Gabriela Bergonzelli¹; Gérard Waeber; Jean-François Aubert; Jürg Nussberger; Haralambos Gavras; Pascal Nicod; Bernard Waeber

From the Department of Internal Medicine B (J.-A.H., G.B., G.W., P.N.) Centre Hospitalier Universitaire Vaudois, the Division of Hypertension (J.-F.A., J.N., B.W.), Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland, and the Hypertension Section (H.G.), Boston University Medical Center, Boston, Mass.

Correspondence to J.-A. Haefliger, PhD, Laboratory of Molecular Biology 19-521, Department of Internal Medicine B, Centre Hospitalier Universitaire Vaudois, CHUV—1011 Lausanne, Switzerland.

Abstract The aim of this investigation was to examine the interrelation between renal mRNA levels of renin and angiotensin II receptor type 1 (AT₁) in a renin-dependent form of experimental hypertension. Rats were studied 4 weeks after unilateral renal artery clipping. Mean blood pressure and plasma renin activity were significantly higher in the hypertensive rats (n=10 206±8 mm Hg and 72.4±20.9 ng · mL^-1 · h^-1, respectively) than in sham-operated controls (n=10, 136±3 mm Hg and 3.3±0.5 ng · mL^-1 · h^-1, respectively). Northern blot analysis of polyA⁺ RNA obtained from the kidneys of renal hypertensive rats showed increased levels of renin mRNA in the clipped kidney, whereas a decrease was observed in the unclipped kidney. Plasma renin activity was directly correlated with renin mRNA expression of the poststenotic kidney (r=.94, P<.01). AT₁ mRNA expression was lower in both kidneys of the hypertensive rats. This downregulation was specific for the AT_1A subtype since the renal expression of the AT_1B subtype remained normal in hypertensive rats. The downregulation of the renal AT_1A receptor may be due to high circulating angiotensin II levels. This is supported by the significant inverse correlation (r=-.71, P<.01) between plasma renin activity and AT_1A mRNA expression measured in the clipped kidney of the hypertensive rats.

Key Words: renin • RNA, messenger • blood pressure • renovascular hypertension • receptors, angiotensin

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