(Hypertension. 1996;27:281-286.)
© 1996 American Heart Association, Inc.
Articles |
Activation of Angiotensin-Generating Systems in the Developing Rat Kidney
Presented in part at the Society for Pediatric Research meeting, May 5, 1994, and published in abstract form in Pediatr Res. 1994;35:363A.
From the Department of Pediatrics, Section of Pediatric Nephrology, Tulane University School of Medicine, New Orleans, La.
Correspondence to Samir S. El-Dahr, MD, Tulane University School of Medicine, Department of Pediatrics, SL-37, 1430 Tulane Ave, New Orleans, LA 70112.
Abstract The present study was designed to determine the developmental changes in intrarenal angiotensin (Ang) peptides in the rat. Kidney Ang I and II levels were threefold and sixfold higher in newborn than adult kidneys, respectively (Ang I, 678±180 versus 243±38 fmol/g, P<.01; Ang II, 667±75 versus 103±6 fmol/g, P<.001). Intrarenal Ang II levels correlated positively with the temporal changes in renin gene expression (r=.93, P<.001). However, no correlation was found between renal Ang II content and angiotensin-converting enzyme (ACE) expression during development, which prompted us to evaluate whether renal enzymes, other than renin and ACE, contribute to Ang II formation in the developing kidney. Angiotensin peptide levels were measured in newborn and adult kidney homogenates incubated with human angiotensinogen (a poor rat renin substrate) for 30 minutes at 37°C. Inhibitors of aspartyl proteases and metalloproteases were ineffective in preventing the formation of Ang II in either newborn or adult kidneys. However, addition of the serine protease inhibitors soybean trypsin inhibitor and phenylmethylsulfonyl fluoride inhibited Ang II generation in the newborn kidneys only. In contrast, Ang I generation was not affected by inhibition of serine proteases in either newborn or adult kidneys. We conclude that Ang I and II synthesis is activated in the developing rat kidney. In addition to renin and ACE, the newborn rat kidney expresses serine protease activity that is capable of generating Ang II directly from angiotensinogen. This putative enzyme is induced in the newborn kidney and may cooperate with renin in the activation of Ang II synthesis during early development.
Key Words: renin-angiotensin system • enzymes • kidney • serine proteinases
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