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(Hypertension. 1996;27:392-398.)
© 1996 American Heart Association, Inc.

Articles

Type 1 Angiotensin II Receptor Subtypes in Kidney of Normal and Salt-Sensitive Hypertensive Rats

Nadine Bouby; Lise Bankir; Catherine Llorens-Cortes

From INSERM U90, Hôpital Necker, and INSERM U36, Collège de France (C.L.-C.), Paris, France.

Correspondence to Nadine Bouby, INSERM U90, Hôpital Necker, 161 rue de Sèvres, 75743 Paris Cedex 15, France.

Abstract We studied the localization and regulation of the two type 1 angiotensin II receptor subtypes AT_1A and AT_1B in different renal zones of the rat kidney by a reverse transcription–polymerase chain reaction amplification method. The yield of the reaction was quantified with an internal standard that was a 63-bp deleted mutant cRNA of the AT_1A receptor. In kidneys of male Sprague-Dawley rats (n=4), the levels of AT_1A and AT_1B receptor mRNAs were highest in the inner stripe of the outer medulla, lowest in the inner medulla, and intermediate in the cortex and outer stripe of the outer medulla. Results (mean±SE) expressed in 10⁵ molecules per microgram total RNA were for cortex, outer stripe, inner stripe, and inner medulla, respectively, 171±15, 152±27, 322±10, and 73±3 for AT_1A, and 35±9, 26±1, 71±10, and 53±11 for AT_1B. In Sabra rats sensitive (n=6) or resistant (n=6) to salt-induced hypertension and maintained on a normal salt diet, the percentage and level of each receptor subtype mRNA in cortex and outer stripe were similar in the two strains and comparable to those observed in Sprague-Dawley rats. However, AT_1A of the inner stripe was significantly decreased in salt-resistant compared with salt-sensitive rats (166±28 and 318±58 10⁵ molecules per microgram total RNA, respectively). These modifications were organ specific because no difference in the level of the receptor mRNAs was observed in the liver of the two Sabra rat strains, whereas a twofold increase in AT_1A mRNA level but not in AT_1B mRNA level was apparent in adrenal and in one renal zone, the inner stripe of the outer medulla, of hypertension-prone Sabra rats.

Key Words: kidney • RT-PCR • angiotensin II receptors • hypertension, sodium-dependent • rats, Sabra

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