(Hypertension. 1996;27:449-455.)
© 1996 American Heart Association, Inc.
Articles |
Localization of 
2A- and 2B-Adrenergic Receptor Subtypes in Brain
From the Hypertension and Atherosclerosis Section of the Department of Medicine and the Department of Anatomy and Neurobiology (D.L.R.), Boston (Mass) University School of Medicine.
Correspondence to Haralambos Gavras, MD, Hypertension and Atherosclerosis Section, Boston University School of Medicine, 80 E Concord St, Boston, MA 02118.
Abstract Recent studies have shown that all three subtypes of
2-adrenergic receptor (2-AR) are found in
brain. The purpose of this study was to map the subtype localization of
the 2A- and 2B-ARs in brain structures.
RNase protection shows that both the 2A- and
2B-ARs are detectable in cortex, cerebellum,
pons-medulla, and hypothalamus. We tested probes derived from the
2A- and 2B-AR cDNAs on cell lines that
express each of the 2-AR subtypes to establish the
subtype specificity of these probes for in situ hybridization. Then we
used the 2A- and 2B-AR probes for in situ
hybridization on sagittal and coronal sections of rat brain. Both
2A and 2B mRNA were detected throughout
the brain. Overall, there appears to be a greater expression of message
for 2A- than 2B-AR in most brain areas,
with the exception of the thalamus. Developing these probes for in situ
hybridization is an important step for further studies on the exact
role of the 2-AR subtypes in neurons that modulate
cardiovascular function.
Key Words: receptors, adrenergic, alpha • brain
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