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(Hypertension. 1996;27:495-501.)
© 1996 American Heart Association, Inc.

Articles

Strategies for Studying Cardiovascular Phenotypes in Genetically Manipulated Mice

K. David Becker; Kim R. Gottshall; Kenneth R. Chien

From the Department of Medicine, Center for Molecular Genetics and American Heart Association Bugher Foundation Center for Molecular Biology, University of California, San Diego, La Jolla.

Abstract Unraveling the pathogenesis of complex cardiovascular diseases, such as hypertension, requires the development of in vivo animal model systems. Although large-animal models have long served as the gold standard, recent advances in transgenic and gene-targeting approaches, mouse genetics, and microsurgical technology are initiating a revolution that has led to the unexpected coupling of in vivo molecular physiology with genetically engineered mice. This article discusses the design of strategies to study complex cardiovascular phenotypes in genetically modified mice, including both transgenic and gene-targeted animals. At this time, a number of strategies are used to address specific molecular or physiological questions, and examples are briefly highlighted. In addition, a number of potential problems in the generation and use of transgenic mice in the study of cardiovascular biology are presented.

Key Words: models, cardiovascular • animals, transgenic • mice, knockout • gene targeting • mice, inbred strains • myocardium

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