(Hypertension. 1996;27:1153-1159.)
© 1996 American Heart Association, Inc.
Articles |
Endothelin Adrenocortical Secretagogue Effect Is Mediated by the B Receptor in Rats
From the Departments of Anatomy (A.S.B., P.G.A., G.N., G.G.N.) and Clinical Medicine (G.P.R., G.A., A.C.P.), School of Medicine, University of Padua (Italy).
Correspondence to Prof G.G. Nussdorfer, Department of Anatomy, Via Gabelli 65, I-35121 Padova, Italy.
Abstract We investigated the gene expression and localization of endothelin-1 (ET-1) receptor subtypes ETA and ETB in the rat adrenal cortex as well as their involvement in the corticosteroid secretagogue effect of ET-1 in vitro. Reverse transcription–polymerase chain reaction with primers specific for ETA and ETB cDNAs demonstrated the expression of both receptor genes in homogenates of adrenocortical tissue. However, in isolated zona glomerulosa and zona fasciculata cells, only ETB mRNA was detected. Autoradiographic examination of the selective displacement of 125I–ET-1 binding by BQ-123 and BQ-788 (specific ligands for ETA and ETB, respectively) indicated that zona glomerulosa possesses both ETA and ETB, whereas zona fasciculata is exclusively provided with ETB. ET-1 enhanced in a concentration-dependent manner aldosterone and corticosterone secretions of dispersed zona glomerulosa and zona fasciculata cells, respectively. The ETB antagonist BQ-788 markedly reduced the secretory response of zona glomerulosa cells and completely suppressed that of zona fasciculata cells, whereas the ETA antagonist BQ-123 was ineffective. These findings indicate that in the rat, the adrenocortical secretagogue action of ET-1 is mediated by the ETB receptor subtype and that the ETA receptor is not directly involved in such an effect.
Key Words: endothelin • receptors, endothelin • adrenergic beta-antagonists • adrenal cortex • steroids • gene expression
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