This Article Full Text Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tamura, K. Articles by Ishii, M. Search for Related Content PubMed PubMed Citation Articles by Tamura, K. Articles by Ishii, M. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Medline Plus Health Information High Blood Pressure

(Hypertension. 1996;27:1216-1223.)
© 1996 American Heart Association, Inc.

Articles

Tissue-Specific Regulation of Angiotensinogen Gene Expression in Spontaneously Hypertensive Rats

Kouichi Tamura; Satoshi Umemura; Nobuo Nyui; Tadashi Yamakawa; Satoshi Yamaguchi; Tomoaki Ishigami; Shun-ichi Tanaka; Keiji Tanimoto; Nobuyoshi Takagi; Hisahiko Sekihara; Kazuo Murakami; Masao Ishii

From the Second (K. Tamura, S.U., N.N., S.Y., T.I., N.T., M.I.) and Third (T.Y., S.T., H.S.) Departments of Internal Medicine, Yokohama City (Japan) University School of Medicine, and Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan (K. Tanimoto, K.M.).

Correspondence to Kouichi Tamura, MD, Second Department of Internal Medicine, Yokohama City University School of Medicine, 3-9, Fukuura, Kanazawa-Ku, Yokohama 236, Japan.

Abstract Angiotensinogen is expressed in many tissues besides the liver. Recent studies have suggested that abnormalities in the regulation of angiotensinogen gene expression may be involved in the development of hypertension. However, little information is available concerning the functional significance of tissue angiotensinogen. In this study, we measured plasma angiotensinogen concentration by radioimmunoassay and examined the expression of tissue angiotensinogen by Northern blot analysis in spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY). Although plasma angiotensinogen concentration in SHR was comparable to that in WKY at 6 weeks of age, it was increased significantly at 14 weeks of age in SHR and became higher than that in WKY. The levels of hepatic angiotensinogen mRNA were similar in SHR and WKY, and the levels of aortic, adrenal, and renal angiotensinogen mRNAs were lower in SHR than in WKY at both 6 and 14 weeks of age. Brain angiotensinogen expression in SHR was higher than in WKY at 6 weeks of age and was comparable to that in WKY at 14 weeks of age. On the other hand, cardiac and fat angiotensinogen mRNA levels were significantly increased at 14 weeks of age in SHR. These results demonstrate that the expression of tissue angiotensinogen is regulated differently in SHR and WKY and indicate that the development of hypertension is accompanied at least temporally with increases in plasma angiotensinogen concentration as well as cardiac and adipogenic angiotensinogen mRNA in SHR.

Key Words: rats, inbred SHR • gene expression • angiotensinogen • renin-angiotensin system • RNA, messenger

This article has been cited by other articles:

				A.-i. Shigenaga, K. Tamura, H. Wakui, S.-i. Masuda, K. Azuma, Y. Tsurumi-Ikeya, M. Ozawa, M. Mogi, M. Matsuda, K. Uchino, et al. Effect of Olmesartan on Tissue Expression Balance Between Angiotensin II Receptor and Its Inhibitory Binding Molecule Hypertension, October 1, 2008; 52(4): 672 - 678. [Abstract] [Full Text] [PDF]

				W. Xiang, J. Kong, S. Chen, L.-P. Cao, G. Qiao, W. Zheng, W. Liu, X. Li, D. G. Gardner, and Y. C. Li Cardiac hypertrophy in vitamin D receptor knockout mice: role of the systemic and cardiac renin-angiotensin systems Am J Physiol Endocrinol Metab, January 1, 2005; 288(1): E125 - E132. [Abstract] [Full Text] [PDF]

				D. O'Regan, C. J. Kenyon, J. R. Seckl, and M. C. Holmes Glucocorticoid exposure in late gestation in the rat permanently programs gender-specific differences in adult cardiovascular and metabolic physiology Am J Physiol Endocrinol Metab, November 1, 2004; 287(5): E863 - E870. [Abstract] [Full Text] [PDF]

				V. Serazin, M.-N. Dieudonne, M. Morot, P. de Mazancourt, and Y. Giudicelli cAMP-positive regulation of angiotensinogen gene expression and protein secretion in rat adipose tissue Am J Physiol Endocrinol Metab, March 1, 2004; 286(3): E434 - E438. [Abstract] [Full Text] [PDF]

				Y. Naito, T. Tsujino, Y. Fujioka, M. Ohyanagi, and T. Iwasaki Augmented Diurnal Variations of the Cardiac Renin-Angiotensin System in Hypertensive Rats Hypertension, December 1, 2002; 40(6): 827 - 833. [Abstract] [Full Text] [PDF]

				L. Dalla Libera, B. Ravara, A. Angelini, K. Rossini, M. Sandri, G. Thiene, G. Battista Ambrosio, and G. Vescovo Beneficial Effects on Skeletal Muscle of the Angiotensin II Type 1 Receptor Blocker Irbesartan in Experimental Heart Failure Circulation, May 1, 2001; 103(17): 2195 - 2200. [Abstract] [Full Text] [PDF]

				A. M Sharma and S. Engeli The renin-angiotensin system in obesity hypertension Journal of Renin-Angiotensin-Aldosterone System, March 1, 2001; 2(1_suppl): S114 - S119. [PDF]

				V. Serazin-Leroy, M. Morot, P. de Mazancourt, and Y. Giudicelli Androgen regulation and site specificity of angiotensinogen gene expression and secretion in rat adipocytes Am J Physiol Endocrinol Metab, December 1, 2000; 279(6): E1398 - E1405. [Abstract] [Full Text] [PDF]

				S. Engeli, R. Negrel, and A. M. Sharma Physiology and Pathophysiology of the Adipose Tissue Renin-Angiotensin System Hypertension, June 1, 2000; 35(6): 1270 - 1277. [Abstract] [Full Text] [PDF]

				A. Reaux, M. C. Fournie-Zaluski, C. David, S. Zini, B. P. Roques, P. Corvol, and C. Llorens-Cortes Aminopeptidase A inhibitors as potential central antihypertensive agents PNAS, November 9, 1999; 96(23): 13415 - 13420. [Abstract] [Full Text] [PDF]

				J. Zicha and J. Kunes Ontogenetic Aspects of Hypertension Development: Analysis in the Rat Physiol Rev, October 1, 1999; 79(4): 1227 - 1282. [Abstract] [Full Text] [PDF]

				R. L. Davisson, G. Yang, T. G. Beltz, M. D. Cassell, A. K. Johnson, and C. D. Sigmund The Brain Renin-Angiotensin System Contributes to the Hypertension in Mice Containing Both the Human Renin and Human Angiotensinogen Transgenes Circ. Res., November 16, 1998; 83(10): 1047 - 1058. [Abstract] [Full Text] [PDF]

				K. Tamura, S. Umemura, N. Nyui, K. Hibi, T. Ishigami, M. Kihara, Y. Toya, and M. Ishii Activation of angiotensinogen gene in cardiac myocytes by angiotensin II and mechanical stretch Am J Physiol Regulatory Integrative Comp Physiol, July 1, 1998; 275(1): R1 - R9. [Abstract] [Full Text] [PDF]

				T. Ishigami, S. Umemura, K. Tamura, K. Hibi, N. Nyui, M. Kihara, M. Yabana, Y. Watanabe, Y. Sumida, T. Nagahara, et al. Essential Hypertension and 5' Upstream Core Promoter Region of Human Angiotensinogen Gene Hypertension, December 1, 1997; 30(6): 1325 - 1330. [Abstract] [Full Text]

				N. Nyui, K. Tamura, S. Yamaguchi, M. Nakamaru, T. Ishigami, M. Yabana, M. Kihara, H. Ochiai, N. Miyazaki, S. Umemura, et al. Tissue Angiotensinogen Gene Expression Induced by Lipopolysaccharide in Hypertensive Rats Hypertension, October 1, 1997; 30(4): 859 - 867. [Abstract] [Full Text]

				K. Tamura, Y. E. Chen, M. Lopez-Ilasaca, L. Daviet, N. Tamura, T. Ishigami, M. Akishita, I. Takasaki, Y. Tokita, R. E. Pratt, et al. Molecular Mechanism of Fibronectin Gene Activation by Cyclic Stretch in Vascular Smooth Muscle Cells J. Biol. Chem., October 27, 2000; 275(44): 34619 - 34627. [Abstract] [Full Text] [PDF]