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(Hypertension. 1996;27:1216-1223.)
© 1996 American Heart Association, Inc.

Articles

Tissue-Specific Regulation of Angiotensinogen Gene Expression in Spontaneously Hypertensive Rats

Kouichi Tamura; Satoshi Umemura; Nobuo Nyui; Tadashi Yamakawa; Satoshi Yamaguchi; Tomoaki Ishigami; Shun-ichi Tanaka; Keiji Tanimoto; Nobuyoshi Takagi; Hisahiko Sekihara; Kazuo Murakami; Masao Ishii

From the Second (K. Tamura, S.U., N.N., S.Y., T.I., N.T., M.I.) and Third (T.Y., S.T., H.S.) Departments of Internal Medicine, Yokohama City (Japan) University School of Medicine, and Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan (K. Tanimoto, K.M.).

Correspondence to Kouichi Tamura, MD, Second Department of Internal Medicine, Yokohama City University School of Medicine, 3-9, Fukuura, Kanazawa-Ku, Yokohama 236, Japan.

Abstract Angiotensinogen is expressed in many tissues besides the liver. Recent studies have suggested that abnormalities in the regulation of angiotensinogen gene expression may be involved in the development of hypertension. However, little information is available concerning the functional significance of tissue angiotensinogen. In this study, we measured plasma angiotensinogen concentration by radioimmunoassay and examined the expression of tissue angiotensinogen by Northern blot analysis in spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY). Although plasma angiotensinogen concentration in SHR was comparable to that in WKY at 6 weeks of age, it was increased significantly at 14 weeks of age in SHR and became higher than that in WKY. The levels of hepatic angiotensinogen mRNA were similar in SHR and WKY, and the levels of aortic, adrenal, and renal angiotensinogen mRNAs were lower in SHR than in WKY at both 6 and 14 weeks of age. Brain angiotensinogen expression in SHR was higher than in WKY at 6 weeks of age and was comparable to that in WKY at 14 weeks of age. On the other hand, cardiac and fat angiotensinogen mRNA levels were significantly increased at 14 weeks of age in SHR. These results demonstrate that the expression of tissue angiotensinogen is regulated differently in SHR and WKY and indicate that the development of hypertension is accompanied at least temporally with increases in plasma angiotensinogen concentration as well as cardiac and adipogenic angiotensinogen mRNA in SHR.

Key Words: rats, inbred SHR • gene expression • angiotensinogen • renin-angiotensin system • RNA, messenger

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