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(Hypertension. 1996;28:98-103.)
© 1996 American Heart Association, Inc.

Articles

Angiotensin I–Converting Enzyme Genotypes and Angiotensin II Receptors

Response to Therapy

Jose L. Dieguez-Lucena; Pedro Aranda-Lara; Maximiliano Ruiz-Galdon; Javier Garcia-Villanova; Miguel Morell-Ocana; Armando Reyes-Engel

Departamento Bioquimica y Biologia Molecular, Facultad de Medicina, Universidad de Malaga, and Hospital Regional Carlos Haya (P.A.-L.), Malaga, Spain.

In the present study, we studied angiotensin II type 1 (AT₁) and type 2 (AT₂) receptor messengers by quantitative reverse transcriptase–polymerase chain reaction. We examined peripheral blood mononuclear cells from 30 healthy subjects and 50 subjects with primary hypertension, in whom angiotensin I–converting enzyme genotype was determined, before and after 15 days of treatment with different antihypertensive drugs. The medication included a calcium channel antagonist, an angiotensin I–converting enzyme inhibitor, and a ß₁-blocker. We also studied the relationship between AT₁ receptor gene expression and biochemical parameters of the renin-angiotensin system. AT₁ receptor messenger levels were positively correlated with plasma renin activity in both normotensive and untreated hypertensive subjects. Increases of this messenger and plasma angiotensin II levels were correlated with the D allele in the same individuals. AT₁ receptor messenger levels decreased significantly with angiotensin I–converting enzyme inhibitor treatment in subjects with the DD genotype, and a significant decrease was observed in subjects with the II and ID genotypes treated with a calcium antagonist. No changes were observed in mRNA with the ß₁-blocker. We conclude that the AT₂ receptor is not expressed in peripheral leukocytes and that AT₁ receptor messenger levels vary in relation to angiotensin I–converting enzyme genotype and pharmacological treatment. These results suggest that angiotensin I–converting enzyme genotype may be an important factor when deciding on antihypertensive therapy in individuals with primary hypertension.

Key Words: receptors, angiotensin II • angiotensin-converting enzyme • antihypertensive therapy • genotype • polymerase chain reaction • RNA

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