(Hypertension. 1996;28:1064-1069.)
© 1996 American Heart Association, Inc.
Articles |
Models of Experimental Hypertension in Mice
the Hypertension and Atherosclerosis Section of the Department of Medicine, Boston (Mass) University School of Medicine.
Correspondence to Haralambos Gavras, MD, Hypertension and Atherosclerosis Section, Boston University School of Medicine, 80 E Concord St, Boston, MA 02118.
Experimental models of hypertension in various animals are useful in the research of vasoactive mechanisms. Recombinant DNA technology has produced genetically engineered animals, mostly mice, useful in hypertension research. However, the development of hypertensive models in mice is fraught with technical difficulties. We describe here the successful development in mice of two common types of experimental hypertension: the renovascular two-kidney, one clip and mineralocorticoid deoxycorticosterone-salt models. By adapting technology previously used in rats, we succeeded in developing hypertension (defined as systolic pressures higher than 140 mm Hg) in more than 50% of mice so treated. We also adapted the methodology for indirect tail-cuff blood pressure measurements as well as for direct intra-arterial monitoring of blood pressure in conscious, freely moving mice. Application of these techniques in transgenic or gene knockout mice with altered vasoactive hormones or receptors should allow elucidation of the role of the target gene products in various types of hypertension.
Key Words: hypertension, renovascular • hypertension, mineralocorticoid • deoxycorticosterone • blood pressure monitoring • mice
This article has been cited by other articles:
 |
|
 |
|
  V. Griol-Charhbili, L. Sabbah, J. Colucci, M.-P. Vincent, V. Baudrie, D. Laude, J.-L. Elghozi, P. Bruneval, N. Picard, P. Meneton, et al. Tissue kallikrein deficiency and renovascular hypertension in the mouse Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2009; 296(5): R1385 - R1391. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yamashita, K. G. Bharadwaj, S. Ikeda, T.-S. Park, and I. J. Goldberg Cardiac metabolic compensation to hypertension requires lipoprotein lipase Am J Physiol Endocrinol Metab, September 1, 2008; 295(3): E705 - E713. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Duka, E. Kintsurashvili, I. Duka, D. Ona, T. A. Hopkins, M. Bader, I. Gavras, and H. Gavras Angiotensin-Converting Enzyme Inhibition After Experimental Myocardial Infarct: Role of the Kinin B1 and B2 Receptors Hypertension, May 1, 2008; 51(5): 1352 - 1357. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Medeiros, N. P. L. Rolim, R. S. F. Oliveira, K. T. Rosa, K. C. Mattos, D. E. Casarini, M. C. Irigoyen, E. M. Krieger, J. E. Krieger, C. E. Negrao, et al. Exercise training delays cardiac dysfunction and prevents calcium handling abnormalities in sympathetic hyperactivity-induced heart failure mice J Appl Physiol, January 1, 2008; 104(1): 103 - 109. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. B. Ferreira, A. V. Bacurau, F. S. Evangelista, M. A. Coelho, E. M. Oliveira, D. E. Casarini, J. E. Krieger, and P. C. Brum The role of local and systemic renin angiotensin system activation in a genetic model of sympathetic hyperactivity-induced heart failure in mice Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2008; 294(1): R26 - R32. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. A. Korshunov, M. Daul, M. P. Massett, and B. C. Berk Axl Mediates Vascular Remodeling Induced by Deoxycorticosterone Acetate Salt Hypertension Hypertension, December 1, 2007; 50(6): 1057 - 1062. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Harris, H. I. Cohn, S. Pesant, R.-H. Zhou, and A. D. Eckhart Vascular smooth muscle Gq signaling is involved in high blood pressure in both induced renal and genetic vascular smooth muscle-derived models of hypertension Am J Physiol Heart Circ Physiol, November 1, 2007; 293(5): H3072 - H3079. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Wagenseil, R. H. Knutsen, D. Y. Li, and R. P. Mecham Elastin-insufficient mice show normal cardiovascular remodeling in 2K1C hypertension despite higher baseline pressure and unique cardiovascular architecture Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H574 - H582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Gavazzi, C. Deffert, C. Trocme, M. Schappi, F. R. Herrmann, and K.-H. Krause NOX1 Deficiency Protects From Aortic Dissection in Response to Angiotensin II Hypertension, July 1, 2007; 50(1): 189 - 196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Ding, A. Chapman, R. Boyd, and H. D. Wang ERK activation contributes to regulation of spontaneous contractile tone via superoxide anion in isolated rat aorta of angiotensin II-induced hypertension Am J Physiol Heart Circ Physiol, June 1, 2007; 292(6): H2997 - H3005. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. P. L. Rolim, A. Medeiros, K. T. Rosa, K. C. Mattos, M. C. Irigoyen, E. M. Krieger, J. E. Krieger, C. E. Negrao, and P. C. Brum Exercise training improves the net balance of cardiac Ca2+ handling protein expression in heart failure Physiol Genomics, May 11, 2007; 29(3): 246 - 252. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-A. S. Duhaney, L. Cui, M. K. Rude, N. K. Lebrasseur, S. Ngoy, D. S. De Silva, D. A. Siwik, R. Liao, and F. Sam Peroxisome Proliferator-Activated Receptor {alpha}-Independent Actions of Fenofibrate Exacerbates Left Ventricular Dilation and Fibrosis in Chronic Pressure Overload Hypertension, May 1, 2007; 49(5): 1084 - 1094. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. V. d'Uscio, L. A. Smith, A. V. Santhanam, D. Richardson, K. A. Nath, and Z. S. Katusic Essential Role of Endothelial Nitric Oxide Synthase in Vascular Effects of Erythropoietin Hypertension, May 1, 2007; 49(5): 1142 - 1148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. C. Kane, A. Behfar, R. B. Dyer, D. F. O'Cochlain, X.-K. Liu, D. M. Hodgson, S. Reyes, T. Miki, S. Seino, and A. Terzic KCNJ11 gene knockout of the Kir6.2 KATP channel causes maladaptive remodeling and heart failure in hypertension Hum. Mol. Genet., August 1, 2006; 15(15): 2285 - 2297. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Karuri, Y. Huang, S. Bodreddigari, C. H. Sutter, B. D. Roebuck, T. W. Kensler, and T. R. Sutter 3H-1,2-Dithiole-3-thione Targets Nuclear Factor {kappa}B to Block Expression of Inducible Nitric-Oxide Synthase, Prevents Hypotension, and Improves Survival in Endotoxemic Rats J. Pharmacol. Exp. Ther., April 1, 2006; 317(1): 61 - 67. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Matsui, S. Xu, K. A. Maitland, R. Mastroianni, J. A. Leopold, D. E. Handy, J. Loscalzo, and R. A. Cohen Glucose-6-Phosphate Dehydrogenase Deficiency Decreases Vascular Superoxide and Atherosclerotic Lesions in Apolipoprotein E-/- Mice Arterioscler Thromb Vasc Biol, April 1, 2006; 26(4): 910 - 916. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Xu, B. Jiang, K. A. Maitland, H. Bayat, J. Gu, J. L. Nadler, S. Corda, G. Lavielle, T. J. Verbeuren, A. Zuccollo, et al. The Thromboxane Receptor Antagonist S18886 Attenuates Renal Oxidant Stress and Proteinuria in Diabetic Apolipoprotein E-Deficient Mice Diabetes, January 1, 2006; 55(1): 110 - 119. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Dikalova, R. Clempus, B. Lassegue, G. Cheng, J. McCoy, S. Dikalov, A. S. Martin, A. Lyle, D. S. Weber, D. Weiss, et al. Nox1 Overexpression Potentiates Angiotensin II-Induced Hypertension and Vascular Smooth Muscle Hypertrophy in Transgenic Mice Circulation, October 25, 2005; 112(17): 2668 - 2676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M.P. Arruda, V. A. Peotta, S. S. Meyrelles, and E. C. Vasquez Evaluation of Vascular Function in Apolipoprotein E Knockout Mice With Angiotensin-Dependent Renovascular Hypertension Hypertension, October 1, 2005; 46(4): 932 - 936. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Matsui, S. Xu, K. A. Maitland, A. Hayes, J. A. Leopold, D. E. Handy, J. Loscalzo, and R. A. Cohen Glucose-6 Phosphate Dehydrogenase Deficiency Decreases the Vascular Response to Angiotensin II Circulation, July 12, 2005; 112(2): 257 - 263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Dworschak, L. V. d'Uscio, D. Breukelmann, and J. D. Hannon Increased tolerance to hypoxic metabolic inhibition and reoxygenation of cardiomyocytes from apolipoprotein E-deficient mice Am J Physiol Heart Circ Physiol, July 1, 2005; 289(1): H160 - H167. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Hosoda, T.-a. Koshimizu, A. Tanoue, Y. Nasa, R. Oikawa, T. Tomabechi, S. Fukuda, H. Shinoura, S. Oshikawa, S. Takeo, et al. Two {alpha}1-Adrenergic Receptor Subtypes Regulating the Vasopressor Response Have Differential Roles in Blood Pressure Regulation Mol. Pharmacol., March 1, 2005; 67(3): 912 - 922. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Schwartz, A. Duka, I. Duka, J. Cui, and H. Gavras Novel targets of ANG II regulation in mouse heart identified by serial analysis of gene expression Am J Physiol Heart Circ Physiol, November 1, 2004; 287(5): H1957 - H1966. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Lazartigues, A. J. Lawrence, F. S. Lamb, and R. L. Davisson Renovascular Hypertension in Mice With Brain-Selective Overexpression of AT1a Receptors Is Buffered by Increased Nitric Oxide Production in the Periphery Circ. Res., September 3, 2004; 95(5): 523 - 531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Whitesall, J. B. Hoff, A. P. Vollmer, and L. G. D'Alecy Comparison of simultaneous measurement of mouse systolic arterial blood pressure by radiotelemetry and tail-cuff methods Am J Physiol Heart Circ Physiol, June 1, 2004; 286(6): H2408 - H2415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Schwartz, A. Duka, E. Triantafyllidi, C. Johns, I. Duka, J. Cui, and H. Gavras Serial analysis of gene expression in mouse kidney following angiotensin II administration Physiol Genomics, December 16, 2003; 16(1): 90 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Duka, A. Duka, E. Kintsurashvili, C. Johns, I. Gavras, and H. Gavras Mechanisms Mediating the Vasoactive Effects of the B1 Receptors of Bradykinin Hypertension, November 1, 2003; 42(5): 1021 - 1025. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Walsh, L. Somody, A. Farrell, B. Zhang, J. Brown, C. Pritchard, M. Vincent, and N. J. Samani Analysis of the Role of the SA Gene in Blood Pressure Regulation by Gene Targeting Hypertension, June 1, 2003; 41(6): 1212 - 1218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Gross and F. C. Luft Exercising Restraint in Measuring Blood Pressure in Conscious Mice Hypertension, April 1, 2003; 41(4): 879 - 881. [Full Text] [PDF]
|
|
|
|
|
|
P. C. Brum, J. Kosek, A. Patterson, D. Bernstein, and B. Kobilka Abnormal cardiac function associated with sympathetic nervous system hyperactivity in mice Am J Physiol Heart Circ Physiol, November 1, 2002; 283(5): H1838 - H1845. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Wang, E. Hummler, J. Nussberger, S. Clement, G. Gabbiani, H. R. Brunner, and M. Burnier Blood Pressure, Cardiac, and Renal Responses to Salt and Deoxycorticosterone Acetate in Mice: Role of Renin Genes J. Am. Soc. Nephrol., June 1, 2002; 13(6): 1509 - 1516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. A. Janssen and J. F. M. Smits Autonomic control of blood pressure in mice: basic physiology and effects of genetic modification Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2002; 282(6): R1545 - R1564. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Kintsurashvili, I. Gavras, C. Johns, and H. Gavras Effects of Antisense Oligodeoxynucleotide Targeting of the {alpha}2B-Adrenergic Receptor Messenger RNA in the Central Nervous System Hypertension, November 1, 2001; 38(5): 1075 - 1080. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Kintsurashvili, I. Duka, I. Gavras, C. Johns, D. Farmakiotis, and H. Gavras Effects of ANG II on bradykinin receptor gene expression in cardiomyocytes and vascular smooth muscle cells Am J Physiol Heart Circ Physiol, October 1, 2001; 281(4): H1778 - H1783. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. A. Peotta, E. C. Vasquez, and S. S. Meyrelles Cardiovascular Neural Reflexes in L-NAME-Induced Hypertension in Mice Hypertension, September 1, 2001; 38(3): 555 - 559. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Duka, E. Kintsurashvili, I. Gavras, C. Johns, M. Bresnahan, and H. Gavras Vasoactive Potential of the B1 Bradykinin Receptor in Normotension and Hypertension Circ. Res., February 16, 2001; 88(3): 275 - 281. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Meneton, I. Ichikawa, T. Inagami, and J. Schnermann Renal physiology of the mouse Am J Physiol Renal Physiol, March 1, 2000; 278(3): F339 - F351. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. A. Janssen, P. J. A. Leenders, and J. F. M. Smits Short-term and long-term blood pressure and heart rate variability in the mouse Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2000; 278(1): R215 - R225. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. P. Makaritsis, C. Johns, I. Gavras, J. D. Altman, D. E. Handy, M. R. Bresnahan, and H. Gavras Sympathoinhibitory Function of the {alpha}2A-Adrenergic Receptor Subtype Hypertension, September 1, 1999; 34(3): 403 - 407. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. P. Makaritsis, D. E. Handy, C. Johns, B. Kobilka, I. Gavras, and H. Gavras Role of the {alpha}2B-Adrenergic Receptor in the Development of Salt-Induced Hypertension Hypertension, January 1, 1999; 33(1): 14 - 17. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Madeddu, A. F. Milia, M. B. Salis, L. Gaspa, W. Gross, A. Lippoldt, and C. Emanueli Renovascular Hypertension in Bradykinin B2-Receptor Knockout Mice Hypertension, September 1, 1998; 32(3): 503 - 509. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Mattson Long-term measurement of arterial blood pressure in conscious mice Am J Physiol Regulatory Integrative Comp Physiol, February 1, 1998; 274(2): R564 - R570. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Franco, S. K. Dubois, R. M. Peshock, and R. V. Shohet Magnetic resonance imaging accurately estimates LV mass in a transgenic mouse model of cardiac hypertrophy Am J Physiol Heart Circ Physiol, February 1, 1998; 274(2): H679 - H683. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. D. Wang, D. G. Johns, S. Xu, and R. A. Cohen Role of superoxide anion in regulating pressor and vascular hypertrophic response to angiotensin II Am J Physiol Heart Circ Physiol, May 1, 2002; 282(5): H1697 - H1702. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. D. Wang, S. Xu, D. G. Johns, Y. Du, M. T. Quinn, A. J. Cayatte, and R. A. Cohen Role of NADPH Oxidase in the Vascular Hypertrophic and Oxidative Stress Response to Angiotensin II in Mice Circ. Res., May 9, 2001; 88(9): 947 - 953. [Abstract] [Full Text] [PDF]
|
|