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(Hypertension. 1997;29:228.)
© 1997 American Heart Association, Inc.

Arthur C. Corcoran Memorial Lecture

Intrarenal Dopamine Production and Distribution in the Rat

Physiological Control of Sodium Excretion

Zhi-Qin Wang; Helmy M. Siragy; Robin A. Felder; Robert M. Carey

From the Departments of Medicine and Pathology (R.A.F.), University of Virginia Health Sciences Center, Charlottesville.

Correspondence to Dr Robert M. Carey, Box 395, University of Virginia Health Sciences Center, Charlottesville, VA 22908

Dopamine (DA), produced by the renal proximal tubule, has been demonstrated as an intrarenal paracrine hormone mediating diuresis and natriuresis. The precise mechanism by which DA exerts its cell-to-cell action is not fully understood. In the present study, renal interstitial fluid (RIF) DA (by in vivo microdialysis) and urinary DA excretion (U_DAV) were compared in anesthetized rats on either normal (0.28% NaCl, NS) or high (4.0% NaCl, HS) sodium balance and in response to acute -L-glutamyl-L-dopa (gludopa) administration. Urine flow (UV) and sodium excretion (U_NaV) in HS were greater than in NS rats. U_DAV was increased in HS compared with NS rats. RIF DA was significantly lower in HS than NS rats. Gludopa at 3, 5, and 7.5 nmol/kg (IV bolus) produced a larger increase in U_DAV than RIF DA. Only the highest dose of gludopa (7.5 nmol/kg), which resulted in a 7.3-fold increase in U_DAV and 1.7-fold increase in RIF DA, was associated with significant diuresis and natriuresis. Cortical and medullary blood flow remained unchanged after gludopa (7.5 nmol/kg) administration, while angiotensin II (100 ng·kg^-1·min^-1) induced significant reduction in cortical and medullary blood flow. Prior bilateral renal denervation did not have a significant effect on basal DA levels (RIF DA and U_DAV) or gludopa-induced DA production or natriuresis and diuresis. These data demonstrated that both chronic sodium loading and acute gludopa administration stimulated renal DA production and release predominantly into the tubule lumen, where DA had a direct tubule action in the control of U_NaV. Renal DA production and its renal effects were not significantly regulated by renal sympathetic nerve activity.

Key Words: dopamine • extracellular space • gludopa • kidney • microdialysis • sodium

Abbreviations: DA = dopamine • gludopa = -L-glutamyl-L-dopa • HS = high-salt diet • NE = norepinephrine • NS = normal-salt diet • RIF = renal interstitial fluid • U_DAV = urinary DA excretion • U_NaV = urinary sodium excretion • UV = urinary flow rate

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