This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wang, Z.-Q. Articles by Carey, R. M. Search for Related Content PubMed PubMed Citation Articles by Wang, Z.-Q. Articles by Carey, R. M.

(Hypertension. 1997;29:228.)
© 1997 American Heart Association, Inc.

Arthur C. Corcoran Memorial Lecture

Intrarenal Dopamine Production and Distribution in the Rat

Physiological Control of Sodium Excretion

Zhi-Qin Wang; Helmy M. Siragy; Robin A. Felder; Robert M. Carey

From the Departments of Medicine and Pathology (R.A.F.), University of Virginia Health Sciences Center, Charlottesville.

Correspondence to Dr Robert M. Carey, Box 395, University of Virginia Health Sciences Center, Charlottesville, VA 22908

Dopamine (DA), produced by the renal proximal tubule, has been demonstrated as an intrarenal paracrine hormone mediating diuresis and natriuresis. The precise mechanism by which DA exerts its cell-to-cell action is not fully understood. In the present study, renal interstitial fluid (RIF) DA (by in vivo microdialysis) and urinary DA excretion (U_DAV) were compared in anesthetized rats on either normal (0.28% NaCl, NS) or high (4.0% NaCl, HS) sodium balance and in response to acute -L-glutamyl-L-dopa (gludopa) administration. Urine flow (UV) and sodium excretion (U_NaV) in HS were greater than in NS rats. U_DAV was increased in HS compared with NS rats. RIF DA was significantly lower in HS than NS rats. Gludopa at 3, 5, and 7.5 nmol/kg (IV bolus) produced a larger increase in U_DAV than RIF DA. Only the highest dose of gludopa (7.5 nmol/kg), which resulted in a 7.3-fold increase in U_DAV and 1.7-fold increase in RIF DA, was associated with significant diuresis and natriuresis. Cortical and medullary blood flow remained unchanged after gludopa (7.5 nmol/kg) administration, while angiotensin II (100 ng·kg^-1·min^-1) induced significant reduction in cortical and medullary blood flow. Prior bilateral renal denervation did not have a significant effect on basal DA levels (RIF DA and U_DAV) or gludopa-induced DA production or natriuresis and diuresis. These data demonstrated that both chronic sodium loading and acute gludopa administration stimulated renal DA production and release predominantly into the tubule lumen, where DA had a direct tubule action in the control of U_NaV. Renal DA production and its renal effects were not significantly regulated by renal sympathetic nerve activity.

Key Words: dopamine • extracellular space • gludopa • kidney • microdialysis • sodium

Abbreviations: DA = dopamine • gludopa = -L-glutamyl-L-dopa • HS = high-salt diet • NE = norepinephrine • NS = normal-salt diet • RIF = renal interstitial fluid • U_DAV = urinary DA excretion • U_NaV = urinary sodium excretion • UV = urinary flow rate

This article has been cited by other articles:

				S. H. Padia, B. A. Kemp, N. L. Howell, J. J. Gildea, S. R. Keller, and R. M. Carey Intrarenal Angiotensin III Infusion Induces Natriuresis and Angiotensin Type 2 Receptor Translocation in Wistar-Kyoto but not in Spontaneously Hypertensive Rats Hypertension, February 1, 2009; 53(2): 338 - 343. [Abstract] [Full Text] [PDF]

				C. Zeng, I. Armando, Y. Luo, G. M. Eisner, R. A. Felder, and P. A. Jose Dysregulation of dopamine-dependent mechanisms as a determinant of hypertension: studies in dopamine receptor knockout mice Am J Physiol Heart Circ Physiol, February 1, 2008; 294(2): H551 - H569. [Abstract] [Full Text] [PDF]

				A. Maurel, O. Spreux-Varoquaux, F. Amenta, S. K. Tayebati, D. Tomassoni, M.-H. Seguelas, A. Parini, and N. Pizzinat Vesicular monoamine transporter 1 mediates dopamine secretion in rat proximal tubular cells Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1592 - F1598. [Abstract] [Full Text] [PDF]

				C. Zeng, Z. Wang, U. Hopfer, L. D. Asico, G. M. Eisner, R. A. Felder, and P. A. Jose Rat Strain Effects of AT1 Receptor Activation on D1 Dopamine Receptors in Immortalized Renal Proximal Tubule Cells Hypertension, October 1, 2005; 46(4): 799 - 805. [Abstract] [Full Text] [PDF]

				M. R. Garrett, H. Meng, J. P. Rapp, and B. Joe Locating a Blood Pressure Quantitative Trait Locus Within 117 kb on the Rat Genome: Substitution Mapping and Renal Expression Analysis Hypertension, March 1, 2005; 45(3): 451 - 459. [Abstract] [Full Text] [PDF]

				C. Zeng, H. Sanada, H. Watanabe, G. M. Eisner, R. A. Felder, and P. A. Jose Functional genomics of the dopaminergic system in hypertension Physiol Genomics, November 17, 2004; 19(3): 233 - 246. [Abstract] [Full Text] [PDF]

				C. Zeng, Y. Luo, L. D. Asico, U. Hopfer, G. M. Eisner, R. A. Felder, and P. A. Jose Perturbation of D1 Dopamine and AT1 Receptor Interaction in Spontaneously Hypertensive Rats Hypertension, October 1, 2003; 42(4): 787 - 792. [Abstract] [Full Text] [PDF]

				P. Bianchi, M.-H. Seguelas, A. Parini, and C. Cambon Activation of Pro-Apoptotic Cascade by Dopamine in Renal Epithelial Cells is Fully Dependent on Hydrogen Peroxide Generation by Monoamine Oxidases J. Am. Soc. Nephrol., April 1, 2003; 14(4): 855 - 862. [Abstract] [Full Text] [PDF]

				R. Efendiev, A. M. Bertorello, R. Zandomeni, A. R. Cinelli, and C. H. Pedemonte Agonist-dependent Regulation of Renal Na+,K+-ATPase Activity Is Modulated by Intracellular Sodium Concentration J. Biol. Chem., March 22, 2002; 277(13): 11489 - 11496. [Abstract] [Full Text] [PDF]

				R. M. Carey Renal Dopamine System: Paracrine Regulator of Sodium Homeostasis and Blood Pressure Hypertension, September 1, 2001; 38(3): 297 - 302. [Abstract] [Full Text] [PDF]

				G. Luippold, M. Beilharz, and B. Muhlbauer Reduction of glomerular hyperfiltration by dopamine D2-like receptor blockade in experimental diabetes mellitus Nephrol. Dial. Transplant., July 1, 2001; 16(7): 1350 - 1356. [Abstract] [Full Text] [PDF]

				E. Feraille and A. Doucet Sodium-Potassium-Adenosinetriphosphatase-Dependent Sodium Transport in the Kidney: Hormonal Control Physiol Rev, January 1, 2001; 81(1): 345 - 418. [Abstract] [Full Text] [PDF]

				Y. Wang, T. J. Berndt, J. M. Gross, M. A. Peterson, M. J. So, and F. G. Knox Effect of inhibition of MAO and COMT on intrarenal dopamine and serotonin and on renal function Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2001; 280(1): R248 - R254. [Abstract] [Full Text] [PDF]

				Z.-Q. Wang, R. A. Felder, and R. M. Carey Selective Inhibition of the Renal Dopamine Subtype D1A Receptor Induces Antinatriuresis in Conscious Rats Hypertension, January 1, 1999; 33(1): 504 - 510. [Abstract] [Full Text] [PDF]

				R. Ozono, D. P. O'Connell, Z.-Q. Wang, A. F. Moore, H. Sanada, R. A. Felder, and R. M. Carey Localization of the Dopamine D1 Receptor Protein in the Human Heart and Kidney Hypertension, September 1, 1997; 30(3): 725 - 729. [Abstract] [Full Text]