(Hypertension. 1997;30:199-202.)
© 1997 American Heart Association, Inc.
Articles |
Congenic Strains for the Blood Pressure Quantitative Trait Locus on Rat Chromosome 2
From the Department of Physiology and Molecular Medicine, Medical College of Ohio, Toledo.
Correspondence to Alan Y. Deng, PhD, Department of Physiology and Molecular Medicine, Medical College of Ohio, 3000 Arlington Ave, PO Box 10008, Toledo, OH 43699-0008. E-mail adeng{at}vortex.mco.edu
Abstract A quantitative trait locus (QTL) for blood pressure was previously detected by linkage analysis in a region of rat chromosome 2 using segregating populations derived from crosses of the Dahl salt-sensitive (Dahl S) rat with rats of the Wistar-Kyoto (WKY) strain or Milan normotensive strain (MNS). Two congenic strains, S.WKY-D2N35/Nep and S.MNS-Adh/D2Mit5, have been constructed by replacing a region of chromosome 2 of the Dahl S rat with the homologous region (ie, low blood pressure QTL allele) of either the WKY or MNS rat, respectively. Systolic pressures of congenic strains S.WKY-D2N35/Nep and S.MNS-Adh/D2Mit5 fed a 2% NaCl diet for 24 days were 44±4.6 and 29±4.5 mm Hg lower, respectively, than that of the comparably treated Dahl S rats. The differences between congenic and Dahl S rats in blood pressure were highly significant (P<.001) and were corroborated by significantly (P<.001) lower ratio of heart weight to body weight in the congenic strains compared with Dahl S rats. The data from two congenic strains combined unequivocally establish the existence of a blood pressure QTL on rat chromosome 2.
Key Words: hypertension, genetic • rats, Dahl salt-sensitive • rats, Milan • rats, Wistar-Kyoto • genetics
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