This Article Full Text Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lippoldt, A. Articles by Luft, F. C. Search for Related Content PubMed PubMed Citation Articles by Lippoldt, A. Articles by Luft, F. C.

(Hypertension. 1997;30:409.)
© 1997 American Heart Association, Inc.

Articles

Nitric Oxide Synthase and Renin-Angiotensin System Gene Expression in Salt-Sensitive and Salt-Resistant Sabra Rats

Andrea Lippoldt; Volkmar Gross; Kerstin Schneider; Anita Hansson; Sophie Nadaud; Wolfgang Schneider; Michael Bader; Chana Yagil; Yoram Yagil; Friedrich C. Luft

From the Franz Volhard Clinic and the Max Delbrück Center for Molecular Medicine (A.L., V.G., K.S., W.S., M.B., F.C.L.), Virchow Klinikum, Humboldt University, Berlin, Germany; Department of Neuroscience, Division of Cellular and Molecular Neurochemistry, Karolinska Institute (A.H.), Stockholm, Sweden; INSERM U358 (S.N.), Paris, France; and Barzilai Medical Center (C.Y., Y.Y.), Ashkelon, Ben Gurion University, Israel.

Correspondence to Friedrich C. Luft, Franz Volhard Clinic, Wiltbergstr 50, 13122 Berlin, Germany. E-mail fcluft{at}mdc-berlin.de

Abstract The molecular mechanisms of salt sensitivity and the contribution of the kidney to salt-induced hypertension in Sabra rats are imperfectly defined. We investigated the expression of the nitric oxide (NO) system (endothelial, inducible, and neural NO synthases) and renin-angiotensin system (renin, angiotensinogen, and angiotensin II type 1A receptor) gene components in the kidneys of SBN/y (salt-resistant) and SBH/y (salt-sensitive) Sabra rat substrains, with and without deoxycorticosterone acetate (DOCA)–salt treatment. We also looked for immunocytochemical evidence of angiotensin II, the effector peptide of the renin-angiotensin system. Inducible and neural NO synthase gene expression values were lower in SBH/y than in SBN/y before and after DOCA-salt treatment. The gene expression level of endothelial NO synthase was not different in SBH/y and SBN/y, either with or without DOCA salt. Renin gene expression was significantly higher in kidneys of SBN/y than in kidneys of SBH/y rats, whereas angiotensinogen gene expression was significantly lower in SBN/y. After DOCA-salt treatment, renin gene expression was strongly suppressed in both strains but more so in SBH/y. Angiotensinogen gene expression, on the other hand, was increased by DOCA salt in SBN/y rats so that the two strains were no longer different. Angiotensin II immunoreactivity was significantly higher in SBN/y than in SBH/y; however, after DOCA salt, immunoreactivity in both strains was no longer detectable. Angiotensin II type 1A receptor gene expression was not different between the two strains, either before or after DOCA-salt administration. We conclude that DOCA salt induced a decrease in the activity of the renin-angiotensin system but did not change NO synthase gene expression in SBH/y and SBN/y. Inducible and neural NO synthase gene expression values were less in SBH/y than in SBN/y, independent of DOCA-salt administration. Thus, the NO system could explain, at least in part, the salt resistance of SBN/y.

Key Words: hypertension, renal • salt • gene expression • renin-angiotensin system • nitric oxide

This article has been cited by other articles:

				S. Engeli, M. Boschmann, P. Frings, L. Beck, J. Janke, J. Titze, F. C. Luft, M. Heer, and J. Jordan Influence of Salt Intake on Renin-Angiotensin and Natriuretic Peptide System Genes in Human Adipose Tissue Hypertension, December 1, 2006; 48(6): 1103 - 1108. [Abstract] [Full Text] [PDF]

				V. Gross, R. Plehm, J. Tank, J. Jordan, A. Diedrich, M. Obst, and F. C. Luft Heart Rate Variability and Baroreflex Function in AT2 Receptor-Disrupted Mice Hypertension, August 1, 2002; 40(2): 207 - 213. [Abstract] [Full Text] [PDF]

				E. C. Fletcher, N. Orolinova, and M. Bader Blood pressure response to chronic episodic hypoxia: the renin-angiotensin system J Appl Physiol, February 1, 2002; 92(2): 627 - 633. [Abstract] [Full Text] [PDF]

				Z. Tahawi, N. Orolinova, I. G. Joshua, M. Bader, and E. C. Fletcher Physiological and Genomic Consequences of Intermittent Hypoxia: Selected Contribution: Altered vascular reactivity in arterioles of chronic intermittent hypoxic rats J Appl Physiol, May 1, 2001; 90(5): 2007 - 2013. [Abstract] [Full Text] [PDF]

				H. Peng, O. A. Carretero, M. E. Alfie, J. A. Masura, and N.-E. Rhaleb Effects of Angiotensin-Converting Enzyme Inhibitor and Angiotensin Type 1 Receptor Antagonist in Deoxycorticosterone Acetate-Salt Hypertensive Mice Lacking Ren-2 Gene Hypertension, March 1, 2001; 37(3): 974 - 980. [Abstract] [Full Text] [PDF]

				E. MERVAALA, B. DEHMEL, V. GROSS, A. LIPPOLDT, J. BOHLENDER, A. F. MILIA, D. GANTEN, and F. C. LUFT Angiotensin-Converting Enzyme Inhibition and AT1 Receptor Blockade Modify the Pressure-Natriuresis Relationship by Additive Mechanisms in Rats with Human Renin and Angiotensinogen Genes J. Am. Soc. Nephrol., August 1, 1999; 10(8): 1669 - 1680. [Abstract] [Full Text]

				V. GROSS, W. SCHNEIDER, W.-H. SCHUNCK, E. MERVAALA, and F. C. LUFT Chronic Effects of Lovastatin and Bezafibrate on Cortical and Medullary Hemodynamics in Deoxycorticosterone Acetate-Salt Hypertensive Mice J. Am. Soc. Nephrol., July 1, 1999; 10(7): 1430 - 1439. [Abstract] [Full Text]

				U. C. Luft, R. Bychkov, M. Gollasch, V. Gross, J.-B. Roullet, D. A. McCarron, C. Ried, F. Hofmann, Y. Yagil, C. Yagil, et al. Farnesol Blocks the L-Type Ca2+ Channel by Targeting the {alpha}1C Subunit Arterioscler Thromb Vasc Biol, April 1, 1999; 19(4): 959 - 966. [Abstract] [Full Text] [PDF]