(Hypertension. 1997;30:687.)
© 1997 American Heart Association, Inc.
Articles |
Renal Identification of Cyclooxygenase-2 in a Subset of Thick Ascending Limb Cells
From Departamento de Ciencias Fisiologicas, Facultad de Ciencias Biologicas, Pontificia Universidad Catolica de Chile, Santiago, Chile (C.P.V., C.C., P.G.), and Department of Inflammatory Diseases Research, GD Searle T3G, St Louis, Missouri (J.L.F.).
Correspondence to Carlos P. Vio, MD, Departamento de Ciencias Fisiologicas, Pontificia Universidad Catolica de Chile, Casilla 114-D, Santiago, Chile. E-mail cvio{at}genes.bio.puc.cl
Abstract The prostaglandin G2/H2 synthase (cyclooxygenase, COX) is a key regulatory enzyme of prostanoid synthesis pathway. The message-encoding COX isoenzymes (constitutive COX-1 and inducible COX-2) have been described in the rat kidney. However, there is scarce information on the localization of COX-2 in the kidney, although it has been recently reported to be localized in the macula densa. The present study was designed to evaluate the localization of COX-2 in adult rat kidneys. Normal rat kidneys (n=10) were fixed in Bouin and were immunostained with specific antibodies against COX-2 by the peroxidase method. The cellular origin of COX-2 was assessed by the immunostaining of serial consecutive sections with antibodies against Na-K-ATPase, Tamm-Horsfall glycoprotein, H-K-ATPase, kallikrein, and macrophages. COX-2 was consistently observed in a subset of tubular cells located in the cortex and in the outer medulla. The staining of serial sections showed that the COX-2+ cells contained both Na-K-ATPase and Tamm-Horsfall, indicating that they corresponded to thick ascending limb (TAL) cells. They were observed at a considerable distance from the corresponding macula densa, although occasionally they were observed close to glomeruli. The COX-2 staining in the TAL cells was not abolished by dexamethasone treatment (1 to 20 mg/kg), suggesting its constitutive expression in normal kidneys. The presence of COX-2 in TAL (a tubular segment postulated to be devoid of COX-1) may contribute to the handling of ions through local production of prostaglandins.
Key Words: cyclooxygenase-2 • rat kidney • COX-2 immunohistochemistry • thick ascending limbs
This article has been cited by other articles:
 |
|
 |
|
  S. Villanueva, C. Cespedes, A. A. Gonzalez, E. Roessler, and C. P. Vio Inhibition of bFGF-receptor type 2 increases kidney damage and suppresses nephrogenic protein expression after ischemic acute renal failure Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2008; 294(3): R819 - R828. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. El-Achkar, Z. Plotkin, B. Marcic, and P. C. Dagher Sepsis induces an increase in thick ascending limb Cox-2 that is TLR4 dependent Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1187 - F1196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Villanueva, C. Cespedes, A. Gonzalez, and C. P. Vio bFGF induces an earlier expression of nephrogenic proteins after ischemic acute renal failure Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2006; 291(6): R1677 - R1687. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Warford-Woolgar, C. Y.-C. Peng, J. Shuhyta, A. Wakefield, D. Sankaran, M. Ogborn, and H. M. Aukema Selectivity of cyclooxygenase isoform activity and prostanoid production in normal and diseased Han:SPRD-cy rat kidneys Am J Physiol Renal Physiol, April 1, 2006; 290(4): F897 - F904. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Rodriguez, P. De la Cerda, E. Collyer, V. Decap, C. P. Vio, and V. Velarde Cyclooxygenase-2 induction by bradykinin in aortic vascular smooth muscle cells Am J Physiol Heart Circ Physiol, January 1, 2006; 290(1): H30 - H36. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Rodriguez, C. P. Vio, P. L. Pedraza, J. C. McGiff, and N. R. Ferreri Bradykinin Regulates Cyclooxygenase-2 in Rat Renal Thick Ascending Limb Cells Hypertension, August 1, 2004; 44(2): 230 - 235. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Sheu, F. M. Ho, K. F. Chao, M. L. Kuo, and S. H. Liu Activation of Phosphoinositide 3-kinase in Response to High Glucose Leads to Regulation of Reactive Oxygen Species-Related Nuclear Factor-{kappa}B Activation and Cyclooxygenase-2 Expression in Mesangial Cells Mol. Pharmacol., July 1, 2004; 66(1): 187 - 196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Campean, F. Theilig, A. Paliege, M. Breyer, and S. Bachmann Key enzymes for renal prostaglandin synthesis: site-specific expression in rodent kidney (rat, mouse) Am J Physiol Renal Physiol, July 1, 2003; 285(1): F19 - F32. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Castrop, J. Klar, C. Wagner, K. Hocherl, and A. Kurtz General inhibition of renocortical cyclooxygenase-2 expression by the renin-angiotensin system Am J Physiol Renal Physiol, March 1, 2003; 284(3): F518 - F524. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Hocherl, F. Dreher, H. Vitzthum, J. Kohler, and A. Kurtz Cyclosporine A Suppresses Cyclooxygenase-2 Expression in the Rat Kidney J. Am. Soc. Nephrol., October 1, 2002; 13(10): 2427 - 2436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Gallardo, N. Olea, and F. V. Sepulveda Distribution of aquaporins in the colon of Octodon degus, a South American desert rodent Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2002; 283(3): R779 - R788. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hocherl, M. C. Kammerl, K. Schumacher, D. Endemann, H. F. Grobecker, and A. Kurtz Role of prostanoids in regulation of the renin-angiotensin-aldosterone system by salt intake Am J Physiol Renal Physiol, August 1, 2002; 283(2): F294 - F301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. T. Botros, M. Laniado-Schwartzman, and N. G. Abraham Regulation of Cyclooxygenase- and Cytochrome P450-Derived Eicosanoids by Heme Oxygenase in the Rat Kidney Hypertension, February 1, 2002; 39(2): 639 - 644. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Llinas, R. Lopez, F. Rodriguez, F. Roig, and F. J. Salazar Role of COX-2-derived metabolites in regulation of the renal hemodynamic response to norepinephrine Am J Physiol Renal Physiol, November 1, 2001; 281(5): F975 - F982. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Wang, S.-J. An, W.-H. Wang, J. C. McGiff, and N. R. Ferreri CaR-mediated COX-2 expression in primary cultured mTAL cells Am J Physiol Renal Physiol, October 1, 2001; 281(4): F658 - F664. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Schnermann Cyclooxygenase-2 and macula densa control of renin secretion Nephrol. Dial. Transplant., September 1, 2001; 16(9): 1735 - 1738. [Full Text] [PDF]
|
|
|
|
 |
|
 F HALTER, A S TARNAWSKI, A SCHMASSMANN, and B M PESKAR Cyclooxygenase 2{---}implications on maintenance of gastric mucosal integrity and ulcer healing: controversial issues and perspectives Gut, September 1, 2001; 49(3): 443 - 453. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Gallardo, L. P. Cid, C. P. Vio, and F. V. Sepulveda Aquaporin-2, a regulated water channel, is expressed in apical membranes of rat distal colon epithelium Am J Physiol Gastrointest Liver Physiol, September 1, 2001; 281(3): G856 - G863. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. G. Leon, J. Marchetti, and C. P. Vio Renal Cyclooxygenase-2: Evidence for Recruitment of Thick Ascending Limb of Henle Cells in Microdissected Nephron Segments Hypertension, September 1, 2001; 38(3): 630 - 634. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. S. Ostrom, C. Gregorian, R. M. Drenan, K. Gabot, B. K. Rana, and P. A. Insel Key role for constitutive cyclooxygenase-2 of MDCK cells in basal signaling and response to released ATP Am J Physiol Cell Physiol, August 1, 2001; 281(2): C524 - C531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. CASTROP, F. SCHWEDA, K. SCHUMACHER, K. WOLF, and A. KURTZ Role of Renocortical Cyclooxygenase-2 for Renal Vascular Resistance and Macula Densa Control of Renin Secretion J. Am. Soc. Nephrol., May 1, 2001; 12(5): 867 - 874. [Abstract] [Full Text]
|
|
|
|
|
|
C. P. VIO, S.-J. AN, C. CESPEDES, J. C. MCGIFF, and N. R. FERRERI Induction of Cyclooxygenase-2 in Thick Ascending Limb Cells by Adrenalectomy J. Am. Soc. Nephrol., April 1, 2001; 12(4): 649 - 658. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Abassi, S. Brodsky, O. Gealekman, I. Rubinstein, A. Hoffman, and J. Winaver Intrarenal expression and distribution of cyclooxygenase isoforms in rats with experimental heart failure Am J Physiol Renal Physiol, January 1, 2001; 280(1): F43 - F53. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Mann, A. Hartner, B. L. Jensen, K. F. Hilgers, K. Hocherl, B. K. Kramer, and A. Kurtz Acute upregulation of COX-2 by renal artery stenosis Am J Physiol Renal Physiol, January 1, 2001; 280(1): F119 - F125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Rodriguez, M. T. Llinas, C. Moreno, and F. J. Salazar Role of Cyclooxygenase-2-Derived Metabolites and NO in Renal Response to Bradykinin Hypertension, January 1, 2001; 37(1): 129 - 134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Llinas, F. Rodriguez, C. Moreno, and F. J. Salazar Role of cyclooxygenase-2-derived metabolites and nitric oxide in regulating renal function Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2000; 279(5): R1641 - R1646. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-F. Cheng, J.-L. Wang, M.-Z. Zhang, James. A. McKanna, and R. C. Harris Nitric oxide regulates renal cortical cyclooxygenase-2 expression Am J Physiol Renal Physiol, July 1, 2000; 279(1): F122 - F129. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Whelton, G. Schulman, C. Wallemark, E. J. Drower, P. C. Isakson, K. M. Verburg, and G. S. Geis Effects of Celecoxib and Naproxen on Renal Function in the Elderly Arch Intern Med, May 22, 2000; 160(10): 1465 - 1470. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-Z. Zhang, R. C. Harris, and J. A. McKanna Regulation of cyclooxygenase-2 (COX-2) in rat renal cortex by adrenal glucocorticoids and mineralocorticoids PNAS, December 21, 1999; 96(26): 15280 - 15285. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ichihara, J. D. Imig, and L. G. Navar Cyclooxygenase-2 Modulates Afferent Arteriolar Responses to Increases in Pressure Hypertension, October 1, 1999; 34(4): 843 - 847. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. L. Sanchez, L. M. Salgado, N. R. Ferreri, and B. Escalante Effect of Cyclooxygenase-2 Inhibition on Renal Function After Renal Ablation Hypertension, October 1, 1999; 34(4): 848 - 853. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. R. Ferreri, S.-J. An, and J. C. McGiff Cyclooxygenase-2 expression and function in the medullary thick ascending limb Am J Physiol Renal Physiol, September 1, 1999; 277(3): F360 - F368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ichihara, J. D. Imig, E. W. Inscho, and L. G. Navar Cyclooxygenase-2 participates in tubular flow-dependent afferent arteriolar tone: interaction with neuronal NOS Am J Physiol Renal Physiol, October 1, 1998; 275(4): F605 - F612. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-L. Wang, H.-F. Cheng, M.-Z. Zhang, James. A. McKanna, and R. C. Harris Selective increase of cyclooxygenase-2 expression in a model of renal ablation Am J Physiol Renal Physiol, October 1, 1998; 275(4): F613 - F622. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Schnermann Juxtaglomerular cell complex in the regulation of renal salt excretion Am J Physiol Regulatory Integrative Comp Physiol, February 1, 1998; 274(2): R263 - R279. [Abstract] [Full Text] [PDF]
|
|