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(Hypertension. 1997;30:725.)
© 1997 American Heart Association, Inc.

Articles

Localization of the Dopamine D₁ Receptor Protein in the Human Heart and Kidney

Ryoji Ozono; Damian P. O’Connell; Zhi-Qin Wang; Allan F. Moore; Hironobu Sanada; Robin A. Felder; Robert M. Carey

From the Departments of Medicine and Pathology, University of Virginia Health Sciences Center (Charlottesville); and the Department of Pharmacology and Therapeutics, University College Cork, Ireland (D.P.O’C.).

Correspondence to Robert M. Carey, MD, Box 395, University of Virginia Health Sciences Center, Charlottesville, Va 22908. E-mail RMC4C{at}virginia.edu

Abstract The dopamine D₁ receptor has recently been identified in the rat heart and kidney. In the present study, using Western blot analysis and light microscopic immunohistochemistry, we examined D₁ receptor protein expression in the human kidney and heart. Antipeptide polyclonal rabbit antiserum was raised against the third extracellular domain of the native receptor and affinity-purified using a protein-A column. Selectivity of the antiserum was validated by recognition of the D₁ receptor expressed in stably transfected LTK^- cells and Sf-9 cells. The immunohistochemical staining for D₁ receptor protein was distributed throughout the atrium and ventricular myocardium and in the coronary vessels. In the kidney, positive immunoreactive signal was detected in the proximal and distal tubules, the collecting ducts, and the large intrarenal vasculature, whereas staining was absent in the juxtaglomerular (JG) cells and the glomeruli. D₁ receptor antiserum preadsorbed against the immunizing peptide did not produce significant staining. In Western blot analysis, a single 55-kD band was detected for the D₁ receptor in membranes from the D₁ receptor transfected Sf-9 cells but not in nontransfected cells. In the heart and kidney, we detected a 55-kD band as well as an additional 40-kD band, which may reflect partial degradation of the receptor protein. These results provide the first evidence for the localization of the dopamine D₁ receptor protein in the human heart and kidney. The similar distribution of this subtype receptor in the human heart and kidney to that in the rat supports the possible (patho)physiological significance of the peripheral dopamine system in humans.

Key Words: immunohistochemistry • dopamine receptors • heart • kidney • human

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