(Hypertension. 1997;30:859-867.)
© 1997 American Heart Association, Inc.
Articles |
Tissue Angiotensinogen Gene Expression Induced by Lipopolysaccharide in Hypertensive Rats
From the Second Department of Internal Medicine, Yokohama City (Japan) University School of Medicine.
Correspondence to Satoshi Umemura, MD, Second Department of Internal Medicine, Yokohama City University School of Medicine, 3-9, Fukuura, Kanazawa-ku, Yokohama 236, Japan.
Abstract There is now convincing evidence that various
tissues express their own tissue renin-angiotensin system,
which may be regulated independently of the systemic
renin-angiotensin system. However, little information is
available on the regulation of the tissue renin-angiotensin
system. We investigated the regulation of tissue
angiotensinogen gene expression with respect to the
development of hypertension. We measured basal and
lipopolysaccharide-stimulated plasma
angiotensinogen concentrations by radioimmunoassay and
examined the expression of tissue angiotensinogen by
Northern blot analysis in spontaneously hypertensive rats (SHR)
and Wistar-Kyoto rats (WKY) at 4 and 13 weeks of age. Basal plasma
angiotensinogen concentration in SHR was comparable to that
in WKY at 4 weeks of age and was significantly higher than that in WKY
at 13 weeks of age. Lipopolysaccharide induced a significant
increase in plasma angiotensinogen concentration in both
WKY and SHR at 4 and 13 weeks of age. At 4 weeks of age, the basal
levels of angiotensinogen mRNA in the liver, fat, adrenal,
and aorta were higher in WKY than in SHR. At 13 weeks of age, the basal
levels of angiotensinogen mRNA in the fat, adrenal, aorta,
spleen, and kidney were higher in WKY than in SHR, while that in the
liver did not differ significantly between the two strains. At 4 weeks
of age, pretreatment with lipopolysaccharide increased the
angiotensinogen mRNA levels in the liver, fat, adrenal, and
aorta in both WKY and SHR. At 13 weeks of age, pretreatment with
lipopolysaccharide increased the angiotensinogen
mRNA levels in the liver, aorta, and adrenal; decreased those in the
spleen; and had no effect in the kidney in both WKY and SHR.
Interestingly, lipopolysaccharide increased the
angiotensinogen mRNA level in fat only in SHR, with no
effect in WKY, at 13 weeks of age. Lipopolysaccharide
stimulated tumor necrosis factor-
mRNA expression in fat of WKY and
SHR, and the increase in tumor necrosis factor- mRNA level in SHR
was significantly greater than that in WKY. Therefore, the increased
tumor necrosis factor- mRNA expression may be involved in the
increased lipopolysaccharide-induced expression of
angiotensinogen gene in fat of SHR at 13 weeks of age.
These data suggest that the transcriptional and probably
posttranscriptional regulation of angiotensinogen mRNA
differs between SHR and WKY, that the regulation of
angiotensinogen gene expression is tissue-specific, and
that the altered expression of the angiotensinogen gene may
be involved in the development of hypertension.
Key Words: rats, inbred SHR • angiotensinogen • renin-angiotensin system • lipopolysaccharide • RNA, messenger • tumor necrosis factor-
This article has been cited by other articles:
 |
|
 |
|
  J. A. Jessup, A. J. Trask, M. C. Chappell, S. Nagata, J. Kato, K. Kitamura, and C. M. Ferrario Localization of the novel angiotensin peptide, angiotensin-(1-12), in heart and kidney of hypertensive and normotensive rats Am J Physiol Heart Circ Physiol, June 1, 2008; 294(6): H2614 - H2618. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Jankowski, R. Vanholder, M. van der Giet, L. Henning, M. Tolle, G. Schonfelder, A. Krakow, S. Karadogan, N. Gustavsson, J. Gobom, et al. Detection of Angiotensin II in Supernatants of Stimulated Mononuclear Leukocytes by Matrix-Assisted Laser Desorption Ionization Time-of-Flight/Time-of-Flight Mass Analysis Hypertension, September 1, 2005; 46(3): 591 - 597. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Shimizu, M. Miyoshi, K. Matsumoto, O. Goto, T. Imoto, and T. Watanabe The Effect of Central Injection of Angiotensin-Converting Enzyme Inhibitor and the Angiotensin Type 1 Receptor Antagonist on the Induction by Lipopolysaccharide of Fever and Brain Interleukin-1{beta} Response in Rats J. Pharmacol. Exp. Ther., March 1, 2004; 308(3): 865 - 873. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Flesch, A. Hoper, L. Dell'Italia, K. Evans, R. Bond, R. Peshock, A. Diwan, T. A. Brinsa, C.-C. Wei, N. Sivasubramanian, et al. Activation and Functional Significance of the Renin-Angiotensin System in Mice With Cardiac Restricted Overexpression of Tumor Necrosis Factor Circulation, August 5, 2003; 108(5): 598 - 604. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Fernandez-Real and W. Ricart Insulin Resistance and Chronic Cardiovascular Inflammatory Syndrome Endocr. Rev., June 1, 2003; 24(3): 278 - 301. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M Sharma and S. Engeli The renin-angiotensin system in obesity hypertension Journal of Renin-Angiotensin-Aldosterone System, March 1, 2001; 2(1_suppl): S114 - S119. [PDF]
|
|
|
|
|
|
Z. Pausova, B. Deslauriers, D. Gaudet, J. Tremblay, T. A. Kotchen, P. Larochelle, A. W. Cowley, and P. Hamet Role of Tumor Necrosis Factor-{alpha} Gene Locus in Obesity and Obesity-Associated Hypertension in French Canadians Hypertension, July 1, 2000; 36(1): 14 - 19. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Engeli, R. Negrel, and A. M. Sharma Physiology and Pathophysiology of the Adipose Tissue Renin-Angiotensin System Hypertension, June 1, 2000; 35(6): 1270 - 1277. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Zinman, A. J. G. Hanley, S. B. Harris, J. Kwan, and I. G. Fantus Circulating Tumor Necrosis Factor-{alpha} Concentrations in a Native Canadian Population with High Rates of Type 2 Diabetes Mellitus J. Clin. Endocrinol. Metab., January 1, 1999; 84(1): 272 - 278. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. Tamura, S. Umemura, N. Nyui, K. Hibi, T. Ishigami, M. Kihara, Y. Toya, and M. Ishii Activation of angiotensinogen gene in cardiac myocytes by angiotensin II and mechanical stretch Am J Physiol Regulatory Integrative Comp Physiol, July 1, 1998; 275(1): R1 - R9. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-M. Fernandez-Real, B. Lainez, J. Vendrell, M. Rigla, A. Castro, G. Penarroja, M. Broch, A. Perez, C. Richart, P. Engel, et al. Shedding of TNF-alpha receptors, blood pressure, and insulin sensitivity in type 2 diabetes mellitus Am J Physiol Endocrinol Metab, April 1, 2002; 282(4): E952 - E959. [Abstract] [Full Text] [PDF]
|
|