(Hypertension. 1997;30:1097-1104.)
© 1997 American Heart Association, Inc.
Articles |
Effects of Chronic Nitric Oxide Synthase Inhibition on Cerebral Arterioles in Rats
From the Department of Pathology, University of Iowa College of Medicine and Cardiovascular Center, Iowa City.
Correspondence to Gary L. Baumbach, MD, Department of Pathology, 105 Medical Laboratories, University of Iowa College of Medicine, Iowa City, IA 52242. E-mail g-baumbach{at}uiowa.edu
Abstract We examined the effects of nitric oxide (NO) synthase inhibition on the structure and mechanics of cerebral arterioles. We measured pressure, diameter, and cross-sectional area of the vessel wall (histologically) in maximally dilated cerebral arterioles in Sprague-Dawley rats that were untreated or treated for 3 months with the NO synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME; 10 mg/kg per day). Treatment with L-NAME increased cerebral arteriolar mean (87±6 versus 42±2 mm Hg, P<.05) and pulse (25±2 versus 13±2 mm Hg, P<.05) pressures, as well as cross-sectional area of the vessel wall (1839±70 versus 1019±58 µm2, P<.05) and external diameter (101±4 versus 87±2 µm, P<.05). These findings suggest that hypertension induced by NO synthase inhibition is accompanied by hypertrophy of the vessel wall and enlargement of cerebral arterioles in rats. To determine the role of cerebral arteriolar pulse pressure in hypertrophy of cerebral arterioles during inhibition of NO synthase, we measured the cross-sectional area of the vessel wall in rats treated with L-NAME that underwent unilateral carotid clipping. Unilateral carotid clipping failed to prevent increases in cross-sectional area of the vessel wall (1507±173 and 1613±148 µm2 in the clip and sham sides, respectively) in rats treated with L-NAME, even though increases in pulse pressure were prevented (16±1 and 27±1 mm Hg in the clip and sham sides, respectively, P<.05). These findings suggest that inhibition of NO synthase may promote hypertrophy of cerebral arterioles independently of increases in arteriolar pulse pressure.
Key Words: L-NAME • hypertrophy • nitric oxide • remodeling
This article has been cited by other articles:
 |
|
 |
|
  M. J. Cipolla, J. Smith, N. Bishop, L. V. Bullinger, and J. A. Godfrey Pregnancy Reverses Hypertensive Remodeling of Cerebral Arteries Hypertension, April 1, 2008; 51(4): 1052 - 1057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Cipolla Cerebrovascular Function in Pregnancy and Eclampsia Hypertension, July 1, 2007; 50(1): 14 - 24. [Full Text] [PDF]
|
|
|
|
 |
|
 G. L. Baumbach, S. P. Didion, and F. M. Faraci Hypertrophy of Cerebral Arterioles in Mice Deficient in Expression of the Gene for CuZn Superoxide Dismutase Stroke, July 1, 2006; 37(7): 1850 - 1855. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Cipolla, N. DeLance, and L. Vitullo Pregnancy Prevents Hypertensive Remodeling of Cerebral Arteries: A Potential Role in the Development of Eclampsia Hypertension, March 1, 2006; 47(3): 619 - 626. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. L. Baumbach, C. D. Sigmund, and F. M. Faraci Structure of Cerebral Arterioles in Mice Deficient in Expression of the Gene for Endothelial Nitric Oxide Synthase Circ. Res., October 15, 2004; 95(8): 822 - 829. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-M. Chillon and G. L. Baumbach Effects of Indapamide, a Thiazide-Like Diuretic, on Structure of Cerebral Arterioles in Hypertensive Rats Hypertension, May 1, 2004; 43(5): 1092 - 1097. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. L. Baumbach, C. D. Sigmund, and F. M. Faraci Cerebral Arteriolar Structure in Mice Overexpressing Human Renin and Angiotensinogen Hypertension, January 1, 2003; 41(1): 50 - 55. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. L. Baumbach, C. D. Sigmund, T. Bottiglieri, and S. R. Lentz Structure of Cerebral Arterioles in Cystathionine {beta}-Synthase-Deficient Mice Circ. Res., November 15, 2002; 91(10): 931 - 937. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nasreen, T. Nabika, H. Shibata, H. Moriyama, K. Yamashita, J. Masuda, and S. Kobayashi T-786C Polymorphism in Endothelial NO Synthase Gene Affects Cerebral Circulation in Smokers: Possible Gene-Environmental Interaction Arterioscler. Thromb. Vasc. Biol., April 1, 2002; 22(4): 605 - 610. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Regrigny, F. Dupuis, J. Atkinson, P. Liminana, E. Scalbert, P. Delagrange, and J.-M. Chillon Cerebral arteriolar structure and function in pinealectomized rats Am J Physiol Heart Circ Physiol, October 1, 2001; 281(4): H1476 - H1480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Ceiler and J. G. R. De Mey Chronic NG-Nitro-L-Arginine Methyl Ester Treatment Does Not Prevent Flow-Induced Remodeling in Mesenteric Feed Arteries and Arcading Arterioles Arterioscler. Thromb. Vasc. Biol., September 1, 2000; 20(9): 2057 - 2063. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Regrigny, J. Atkinson, C. Capdeville-Atkinson, P. Liminana, and J.-M. Chillon Effect of Lovastatin on Cerebral Circulation in Spontaneously Hypertensive Rats Hypertension, May 1, 2000; 35(5): 1105 - 1110. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-M. Chillon and G. L. Baumbach Effects of an Angiotensin-Converting Enzyme Inhibitor and a ß-Blocker on Cerebral Arterioles in Rats Hypertension, March 1, 1999; 33(3): 856 - 861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Moreau, H. Takase, L. V. d'Uscio, T. F. Luscher, and G. L. Baumbach Effect of Chronic Nitric Oxide Deficiency on Angiotensin II–Induced Hypertrophy of Rat Basilar Artery • Editorial Comment Stroke, May 1, 1998; 29(5): 1031 - 1036. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Nasreen, T. Nabika, H. Shibata, H. Moriyama, K. Yamashita, J. Masuda, and S. Kobayashi T-786C Polymorphism in Endothelial NO Synthase Gene Affects Cerebral Circulation in Smokers: Possible Gene-Environmental Interaction Arterioscler. Thromb. Vasc. Biol., April 1, 2002; 22(4): 605 - 610. [Abstract] [Full Text] [PDF]
|
|