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(Hypertension. 1997;30:1284-1288.)
© 1997 American Heart Association, Inc.

Articles

Increased Expression of Parathyroid Hormone–Related Peptide Gene in Blood Vessels of Spontaneously Hypertensive Rats

Masakuni Noda; Tetsuo Katoh; Kiyoshi Kurokawa; Yoh Takuwa

From the Departments of Cardiovascular Biology (M.N., Y.T.) and Internal Medicine (M.N., T.K., K.K.), Faculty of Medicine, University of Tokyo (Japan).

Correspondence to Yoh Takuwa, MD, Department of Car- diovascular Biology, University of Tokyo Faculty of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, Japan. E-mail yohtakwa{at}m.u-tokyo.ac.jp

Abstract We have shown recently that mechanical stretch of cultured rat aortic smooth muscle cells induces a marked increase in gene expression of the vasorelaxant parathyroid hormone–related peptide. In the present study, we investigated whether mechanical force affected the in vivo parathyroid hormone–related peptide gene expression in blood vessels. Northern blot analysis revealed that stretch of isolated rat aortic strips increased the expression level of parathyroid hormone–related peptide mRNA. The parathyroid hormone–related peptide transcript level in aorta and mesenteric vessels from 18-week-old spontaneously hypertensive rats (SHR) was 2.5- and 2.2-fold higher, respectively, compared with age-matched Wistar-Kyoto (WKY) controls, whereas the parathyroid hormone–related peptide mRNA level in aorta from normotensive 4-week-old SHR was similar to that of age-matched WKY controls. The aortic parathyroid hormone–related peptide content was higher in 18-week-old SHR than in age-matched WKY controls. Moreover, treatment of mature SHR with an angiotensin II type 1 receptor antagonist or hydralazine caused a concomitant decrease in the parathyroid hormone–related peptide transcript level in aorta with lowering of blood pressure. These results suggest that the in vivo parathyroid hormone–related peptide gene expression in blood vessels is under the control of mechanical force, pointing to a role of parathyroid hormone–related peptide in the regulation of vascular tone.

Key Words: rats, inbred SHR • mechanical force • peptides • parathyroid hormone

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