| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
(Hypertension. 1997;30:1628-1633.)
© 1997 American Heart Association, Inc.
Articles |
Oxidative Stress in the Dahl Hypertensive Rat
From the Department of Bioengineering and the Institute for Biomedical Engineering, University of California at San Diego (La Jolla).
Correspondence to Dr Geert W. Schmid-Schönbein, Institute for Biomedical Engineering, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093-0412. E-mail gwss{at}bioeng.ucsd.edu.
Abstract Enhanced production of oxygen free radicals may play a role in hypertension by affecting vascular smooth muscle contraction, resistance to blood flow, and organ damage. The aim of this study was to determine whether oxygen free radicals are involved in the development of salt-induced hypertension. Dahl salt-sensitive (Dahl-S) and salt-resistant (Dahl-R) rats were fed either a high salt (6.0% NaCl) or low salt (0.3% NaCl) diet for 4 weeks. The high salt diet caused the development of severe hypertension in Dahl-S animals and had no effect on blood pressure in Dahl-R animals. A tetranitroblue tetrazolium dye was used to detect superoxide radicals in microvessels of the mesentery. Light absorption measurements revealed enhanced staining along the endothelium of arterioles and venules in hypertensive Dahl-S animals, with significantly lower values in normotensive animals. In addition, a Clark electrochemical electrode was used to measure hydrogen peroxide levels in fresh plasma. Hypertensive Dahl-S animals had a higher plasma hydrogen peroxide concentration compared with their normotensive counterparts (2.81±0.43 versus 2.10±0.41 µmol/L), while no difference was detected between high- and low salt–treated Dahl-R animals (1.70±0.35 versus 1.56±0.51 µmol/L). The plasma hydrogen peroxide levels of all groups correlated with mean arterial pressure (r=.77). These findings demonstrate an enhanced production of oxygen free radicals in the microvasculature of hypertensive Dahl-S rats.
Key Words: hypertension, salt-sensitive • free radicals • nitroblue tetrazolium • hydrogen peroxide • superoxides • rats, Dahl
This article has been cited by other articles:
 |
|
 |
|
  M. Kayikcioglu, M. Tumuklu, M. Ozkahya, O. Ozdogan, G. Asci, S. Duman, H. Toz, L. H. Can, A. Basci, and E. Ok The benefit of salt restriction in the treatment of end-stage renal disease by haemodialysis Nephrol. Dial. Transplant., March 1, 2009; 24(3): 956 - 962. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Macarthur, T. C. Westfall, and G. H. Wilken Oxidative stress attenuates NO-induced modulation of sympathetic neurotransmission in the mesenteric arterial bed of spontaneously hypertensive rats Am J Physiol Heart Circ Physiol, January 1, 2008; 294(1): H183 - H189. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Banday, A. B. Muhammad, F. R. Fazili, and M. Lokhandwala Mechanisms of Oxidative Stress-Induced Increase in Salt Sensitivity and Development of Hypertension in Sprague-Dawley Rats Hypertension, March 1, 2007; 49(3): 664 - 671. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Erdei, Z. Bagi, I. Edes, G. Kaley, and A. Koller H2O2 increases production of constrictor prostaglandins in smooth muscle leading to enhanced arteriolar tone in Type 2 diabetic mice Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H649 - H656. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-W. Gu, N. Tian, M. Shparago, W. Tan, A. P. Bailey, and R. D. Manning Jr. Renal NF-{kappa}B activation and TNF-{alpha} upregulation correlate with salt-sensitive hypertension in Dahl salt-sensitive rats Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2006; 291(6): R1817 - R1824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Matsui, T. Shimosawa, Y. Uetake, H. Wang, S. Ogura, T. Kaneko, J. Liu, K. Ando, and T. Fujita Protective Effect of Potassium Against the Hypertensive Cardiac Dysfunction: Association With Reactive Oxygen Species Reduction Hypertension, August 1, 2006; 48(2): 225 - 231. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Agarwal, K. C. Nandipati, R. K. Sharma, C. D. Zippe, and R. Raina Role of Oxidative Stress in the Pathophysiological Mechanism of Erectile Dysfunction J Androl, May 1, 2006; 27(3): 335 - 347. [Full Text] [PDF]
|
|
|
|
|
|
N. E. Taylor and A. W. Cowley Jr. Effect of renal medullary H2O2 on salt-induced hypertension and renal injury Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2005; 289(6): R1573 - R1579. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Wilcox Oxidative stress and nitric oxide deficiency in the kidney: a critical link to hypertension? Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2005; 289(4): R913 - R935. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Kobayashi, F. A. DeLano, and G. W. Schmid-Schonbein Oxidative Stress Promotes Endothelial Cell Apoptosis and Loss of Microvessels in the Spontaneously Hypertensive Rats Arterioscler Thromb Vasc Biol, October 1, 2005; 25(10): 2114 - 2121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhang, K. K. Griendling, A. Dikalova, G. K. Owens, and W. R. Taylor Vascular Hypertrophy in Angiotensin II-Induced Hypertension Is Mediated by Vascular Smooth Muscle Cell-Derived H2O2 Hypertension, October 1, 2005; 46(4): 732 - 737. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Vasdev, V. Gill, S. Parai, and V. Gadag Dietary Vitamin E Supplementation Attenuates Hypertension in Dahl Salt-Sensitive Rats Journal of Cardiovascular Pharmacology and Therapeutics, April 1, 2005; 10(2): 103 - 111. [Abstract] [PDF]
|
|
|
|
 |
|
 L. L. Howard, M. E. Patterson, J. J. Mullins, and K. D. Mitchell Salt-sensitive hypertension develops after transient induction of ANG II-dependent hypertension in Cyp1a1-Ren2 transgenic rats Am J Physiol Renal Physiol, April 1, 2005; 288(4): F810 - F815. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Drenjancevic-Peric and J. H. Lombard Reduced Angiotensin II and Oxidative Stress Contribute to Impaired Vasodilation in Dahl Salt-Sensitive Rats on Low-Salt Diet Hypertension, April 1, 2005; 45(4): 687 - 691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. A. DeLano, R. Balete, and G. W. Schmid-Schonbein Control of oxidative stress in microcirculation of spontaneously hypertensive rats Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H805 - H812. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Williams, J. S. Pollock, and D. M. Pollock Arterial Pressure Response to the Antioxidant Tempol and ETB Receptor Blockade in Rats on a High-Salt Diet Hypertension, November 1, 2004; 44(5): 770 - 775. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Cseko, Z. Bagi, and A. Koller Biphasic effect of hydrogen peroxide on skeletal muscle arteriolar tone via activation of endothelial and smooth muscle signaling pathways J Appl Physiol, September 1, 2004; 97(3): 1130 - 1137. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Drenjancevic-Peric and J. H. Lombard Introgression of chromosome 13 in Dahl salt-sensitive genetic background restores cerebral vascular relaxation Am J Physiol Heart Circ Physiol, August 1, 2004; 287(2): H957 - H962. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. Fries, P. Mahboubi, N. R. Mahapatra, S. K. Mahata, N. J. Schork, G. W. Schmid-Schoenbein, and D. T. O'Connor Neuroendocrine Transcriptome in Genetic Hypertension: Multiple Changes in Diverse Adrenal Physiological Systems Hypertension, June 1, 2004; 43(6): 1301 - 1311. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kishi, Y. Hirooka, Y. Kimura, K. Ito, H. Shimokawa, and A. Takeshita Increased Reactive Oxygen Species in Rostral Ventrolateral Medulla Contribute to Neural Mechanisms of Hypertension in Stroke-Prone Spontaneously Hypertensive Rats Circulation, May 18, 2004; 109(19): 2357 - 2362. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. K Shieh, E. Kotlyar, and F. Sam Aldosterone and cardiovascular remodelling: focus on myocardial failure Journal of Renin-Angiotensin-Aldosterone System, March 1, 2004; 5(1): 3 - 13. [Abstract] [PDF]
|
|
|
|
 |
|
 M. Galinanes and A. G Fowler Role of clinical pathologies in myocardial injury following ischaemia and reperfusion Cardiovasc Res, February 15, 2004; 61(3): 512 - 521. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Nishiyama, M. Yoshizumi, H. Hitomi, S. Kagami, S. Kondo, A. Miyatake, M. Fukunaga, T. Tamaki, H. Kiyomoto, M. Kohno, et al. The SOD Mimetic Tempol Ameliorates Glomerular Injury and Reduces Mitogen-Activated Protein Kinase Activity in Dahl Salt-Sensitive Rats J. Am. Soc. Nephrol., February 1, 2004; 15(2): 306 - 315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhu, T. Mori, T. Huang, and J. H. Lombard Effect of high-salt diet on NO release and superoxide production in rat aorta Am J Physiol Heart Circ Physiol, February 1, 2004; 286(2): H575 - H583. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Vidrio, M. Medina, P. Gonzalez-Romo, M. Lorenzana-Jimenez, P. Diaz-Arista, and A. Baeza Semicarbazide-Sensitive Amine Oxidase Substrates Potentiate Hydralazine Hypotension: Possible Role of Hydrogen Peroxide J. Pharmacol. Exp. Ther., November 1, 2003; 307(2): 497 - 504. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Fujii, L. Zhang, J. Igarashi, and H. Kosaka L-Arginine Reverses p47phox and gp91phox Expression Induced by High Salt in Dahl Rats Hypertension, November 1, 2003; 42(5): 1014 - 1020. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. D. Dobrian, S. D. Schriver, T. Lynch, and R. L. Prewitt Effect of salt on hypertension and oxidative stress in a rat model of diet-induced obesity Am J Physiol Renal Physiol, October 1, 2003; 285(4): F619 - F628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. E. Callera, R. M. Touyz, S. A. Teixeira, M. N. Muscara, M. H. C. Carvalho, Z. B. Fortes, D. Nigro, E. L. Schiffrin, and R. C. Tostes ETA Receptor Blockade Decreases Vascular Superoxide Generation in DOCA-Salt Hypertension Hypertension, October 1, 2003; 42(4): 811 - 817. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tepel Oxidative stress: does it play a role in the genesis of essential hypertension and hypertension of uraemia? Nephrol. Dial. Transplant., August 1, 2003; 18(8): 1439 - 1442. [Full Text] [PDF]
|
|
|
|
|
|
M. Tepel Oxidative stress: does it play a role in the genesis of essential hypertension and hypertension of uraemia? Nephrol. Dial. Transplant., August 1, 2003; 18(88): 1439 - 1442. [Full Text]
|
|
|
|
|
|
A. Makino, M. M. Skelton, A.-P. Zou, and A. W. Cowley Jr Increased Renal Medullary H2O2 Leads to Hypertension Hypertension, July 1, 2003; 42(1): 25 - 30. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Meng, G. W. Cason, A. W. Gannon, L. C. Racusen, and R. D. Manning Jr Oxidative Stress in Dahl Salt-Sensitive Hypertension Hypertension, June 1, 2003; 41(6): 1346 - 1352. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Chu, S. Iida, D. D. Lund, R. M. Weiss, G. F. DiBona, Y. Watanabe, F. M. Faraci, and D. D. Heistad Gene Transfer of Extracellular Superoxide Dismutase Reduces Arterial Pressure in Spontaneously Hypertensive Rats: Role of Heparin-Binding Domain Circ. Res., March 7, 2003; 92(4): 461 - 468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Hoagland, K. G. Maier, and R. J. Roman Contributions of 20-HETE to the Antihypertensive Effects of Tempol in Dahl Salt-Sensitive Rats Hypertension, March 1, 2003; 41(3): 697 - 702. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Rodriguez-Iturbe, C.-D. Zhan, Y. Quiroz, R. K. Sindhu, and N. D. Vaziri Antioxidant-Rich Diet Relieves Hypertension and Reduces Renal Immune Infiltration in Spontaneously Hypertensive Rats Hypertension, February 1, 2003; 41(2): 341 - 346. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Sun, J. Zhang, L. Lu, S. S. Chen, M. T. Quinn, and K. T. Weber Aldosterone-Induced Inflammation in the Rat Heart : Role of Oxidative Stress Am. J. Pathol., November 1, 2002; 161(5): 1773 - 1781. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Meng, L. J. Roberts II, G. W. Cason, T. S. Curry, and R. D. Manning Jr. Superoxide dismutase and oxidative stress in Dahl salt-sensitive and -resistant rats Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2002; 283(3): R732 - R738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. J. Zhou, N. D. Vaziri, X. Q. Wang, F. G. Silva, and Z. Laszik Nitric Oxide Synthase Expression in Hypertension Induced by Inhibition of Glutathione Synthase J. Pharmacol. Exp. Ther., March 1, 2002; 300(3): 762 - 767. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rathaus and J. Bernheim Oxygen species in the microvascular environment: regulation of vascular tone and the development of hypertension Nephrol. Dial. Transplant., February 1, 2002; 17(2): 216 - 221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Lenda and M. A. Boegehold Effect of a high-salt diet on oxidant enzyme activity in skeletal muscle microcirculation Am J Physiol Heart Circ Physiol, February 1, 2002; 282(2): H395 - H402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-L. Ma, F. Gao, A. H. Nelson, B. L. Lopez, T. A. Christopher, T.-L. Yue, and F. C. Barone Oxidative Inactivation of Nitric Oxide and Endothelial Dysfunction in Stroke-Prone Spontaneous Hypertensive Rats J. Pharmacol. Exp. Ther., September 1, 2001; 298(3): 879 - 885. [Abstract] [Full Text]
|
|
|
|
|
|
A. D. Dobrian, S. D. Schriver, and R. L. Prewitt Role of Angiotensin II and Free Radicals in Blood Pressure Regulation in a Rat Model of Renal Hypertension Hypertension, September 1, 2001; 38(3): 361 - 366. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. D. Vaziri, Y. Ding, and Z. Ni Compensatory Up-Regulation of Nitric-Oxide Synthase Isoforms in Lead-Induced Hypertension; Reversal by a Superoxide Dismutase-Mimetic Drug J. Pharmacol. Exp. Ther., August 1, 2001; 298(2): 679 - 685. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-P. Zou, N. Li, and A. W. Cowley Jr. Production and Actions of Superoxide in the Renal Medulla Hypertension, February 1, 2001; 37(2): 547 - 553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. D. Dobrian, M. J. Davies, S. D. Schriver, T. J. Lauterio, and R. L. Prewitt Oxidative Stress in a Rat Model of Obesity-Induced Hypertension Hypertension, February 1, 2001; 37(2): 554 - 560. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Beswick, H. Zhang, D. Marable, J. D. Catravas, W. D. Hill, and R. C. Webb Long-Term Antioxidant Administration Attenuates Mineralocorticoid Hypertension and Renal Inflammatory Response Hypertension, February 1, 2001; 37(2): 781 - 786. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. V. d'Uscio, T. Quaschning, J. C. Burnett Jr, and T. F. Luscher Vasopeptidase Inhibition Prevents Endothelial Dysfunction of Resistance Arteries in Salt-Sensitive Hypertension in Comparison With Single ACE Inhibition Hypertension, January 1, 2001; 37(1): 28 - 33. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. D. Vaziri, Z. Ni, F. Oveisi, and D. L. Trnavsky-Hobbs Effect of Antioxidant Therapy on Blood Pressure and NO Synthase Expression in Hypertensive Rats Hypertension, December 1, 2000; 36(6): 957 - 964. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Lacy, M. T. Kailasam, D. T. O'Connor, G. W. Schmid-Schonbein, and R. J. Parmer Plasma Hydrogen Peroxide Production in Human Essential Hypertension : Role of Heredity, Gender, and Ethnicity Hypertension, November 1, 2000; 36(5): 878 - 884. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Sauls and M. A. Boegehold Arteriolar wall PO2 and nitric oxide release during sympathetic vasoconstriction in the rat intestine Am J Physiol Heart Circ Physiol, August 1, 2000; 279(2): H484 - H491. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Lenda, B. A. Sauls, and M. A. Boegehold Reactive oxygen species may contribute to reduced endothelium-dependent dilation in rats fed high salt Am J Physiol Heart Circ Physiol, July 1, 2000; 279(1): H7 - H14. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ueno, P. Kanellakis, A. Agrotis, and A. Bobik Blood Flow Regulates the Development of Vascular Hypertrophy, Smooth Muscle Cell Proliferation, and Endothelial Cell Nitric Oxide Synthase in Hypertension Hypertension, July 1, 2000; 36(1): 89 - 96. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. D. Vaziri, X. Q. Wang, F. Oveisi, and B. Rad Induction of Oxidative Stress by Glutathione Depletion Causes Severe Hypertension in Normal Rats Hypertension, July 1, 2000; 36(1): 142 - 146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. D. Dobrian, M. J. Davies, R. L. Prewitt, and T. J. Lauterio Development of Hypertension in a Rat Model of Diet-Induced Obesity Hypertension, April 1, 2000; 35(4): 1009 - 1015. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Griendling, D. Sorescu, and M. Ushio-Fukai NAD(P)H Oxidase : Role in Cardiovascular Biology and Disease Circ. Res., March 17, 2000; 86(5): 494 - 501. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Fujiwara, T. Osanai, T. Kamada, T. Katoh, K. Takahashi, and K. Okumura Study on the Relationship Between Plasma Nitrite and Nitrate Level and Salt Sensitivity in Human Hypertension : Modulation of Nitric Oxide Synthesis by Salt Intake Circulation, February 29, 2000; 101(8): 856 - 861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Kurose, R. Wolf, W. Cerwinka, and D. N. Granger Microvascular Responses to Ischemia/Reperfusion in Normotensive and Hypertensive Rats Hypertension, August 1, 1999; 34(2): 212 - 216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Tritto and G. Ambrosio Spotlight on microcirculation: an update Cardiovasc Res, June 1, 1999; 42(3): 600 - 606. [Full Text] [PDF]
|
|
|
|
 |
|
 R. Ross Atherosclerosis -- An Inflammatory Disease N. Engl. J. Med., January 14, 1999; 340(2): 115 - 126. [Full Text] [PDF]
|
|
|
|
|
|
T. Marumo, V. B. Schini-Kerth, R. P. Brandes, and R. Busse Glucocorticoids Inhibit Superoxide Anion Production and p22 Phox mRNA Expression in Human Aortic Smooth Muscle Cells Hypertension, December 1, 1998; 32(6): 1083 - 1088. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Huang, D. Sun, G. Kaley, and A. Koller Superoxide Released to High Intra-arteriolar Pressure Reduces Nitric Oxide–Mediated Shear Stress– and Agonist-Induced Dilations Circ. Res., November 2, 1998; 83(9): 960 - 965. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Laakso, E. Mervaala, J.-J. Himberg, T.-L. Teravainen, H. Karppanen, H. Vapaatalo, and R. Lapatto Increased Kidney Xanthine Oxidoreductase Activity in Salt-Induced Experimental Hypertension Hypertension, November 1, 1998; 32(5): 902 - 906. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. D. Wang, D. G. Johns, S. Xu, and R. A. Cohen Role of superoxide anion in regulating pressor and vascular hypertrophic response to angiotensin II Am J Physiol Heart Circ Physiol, May 1, 2002; 282(5): H1697 - H1702. [Abstract] [Full Text] [PDF]
|
|