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(Hypertension. 1997;30:1634-1639.)
© 1997 American Heart Association, Inc.

Articles

Fatty Acids Augment Endothelium-Dependent Dilation in Hand Veins by a Cyclooxygenase-Dependent Mechanism

Konrad T. Stepniakowski; Gang Lu; Rajesh K. Davda; ; Brent M. Egan

From the Division of Clinical Pharmacology, Department of Medicine and Pharmacology, Medical University of South Carolina (Charleston).

Abstract Evidence supports the hypothesis that elevated nonesterified fatty acids (NEFAs) in patients with insulin resistance, eg, obese hypertensive subjects, contribute to increased vascular -adrenergic reactivity and tone by impairing endothelium-dependent vasodilation. To generate further support for this notion, we studied responses to endothelium-dependent and independent dilators under control (0.9% NaCl/heparin) conditions in one hand and with elevated NEFAs in the contralateral hand (10% intralipid/heparin). To observe venodilator responses, the dorsal hand vein diameter was first reduced by ~60% with phenylephrine. Studies were repeated with indomethacin to block the generation of cyclooxygenase products. In contrast to previous in vitro data, elevating NEFAs locally in vivo augmented rather than suppressed venodilator responses to the two endothelium-dependent dilators acetylcholine and methacholine (P<.05). Responses to the endothelium-independent dilator nitroglycerin were unaffected. Indomethacin attenuated the capacity of intralipid/heparin to enhance endothelium-dependent dilator responses to acetylcholine and methacholine. Indomethacin did not affect venodilator responses to nitroglycerin. The effect of intralipid/heparin to significantly reduce the phenylephrine infusion rate required to reduce hand vein diameter by ~60% was reversed by indomethacin. These data indicate that raising fatty acids locally augments endothelium-dependent dilation by a cyclooxygenase-dependent mechanism. The findings also suggest that NEFAs augment ₁-adrenoceptor–mediated constriction in hand veins by a cyclooxygenase-dependent mechanism. These hand vein studies do not support the notion that the elevated NEFAs in obese hypertensive patients augment ₁-adrenoceptor–mediated reactivity by reducing nitric oxide synthesis.

Key Words: fatty acids, nonesterified • nitric oxide • indomethacin • adrenoceptors • phenylephrine • vasodilation

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