(Hypertension. 1998;31:271.)
© 1998 American Heart Association, Inc.
Scientific Contributions |
Protective Effect of Angiotensin II-Induced Increase in Nitric Oxide in the Renal Medullary Circulation
From the Department of Physiology, Medical College of Wisconsin, Milwaukee, Wis.
Correspondence to Ai-Ping Zou, MD, PhD, Department of Physiology, Medical College of Wisconsin, 8701; Watertown Plank Road, Milwaukee, WI 53226. E-mail azou{at}post.its.mcw.edu
This study examined the effect of intravenous infusion of subpressor doses of angiotensin (Ang II) on renal medullary blood flow (MBF), medullary partial oxygen pressure (Po2), and nitric oxide (NO) concentration under normal conditions and during reduction of the medullary nitric oxide synthase (NOS) activity in anesthetized rats. With laser Doppler flowmetry and polarographic measurement of Po2 with microelectrodes, Ang II (5 ng/kg per minute) did not alter renal cortical and medullary blood flows or medullary Po2. No-nitro-L-arginine methyl ester (L-NAME) was infused into the renal medullary interstitial space at a dose of 1.4 µg/kg per minute, a dose that did not significantly alter basal levels of MBF or Po2. Intravenous infusion of Ang II at the same dose in the presence of L-NAME decreased MBF by 23% and medullary Po2 by 28%, but it had no effect on cortical blood flow or arterial blood pressure. An in vivo microdialysis-oxyhemoglobin NO trapping technique was used in other rats to determine tissue NO concentrations using the same protocol. Ang II infusion increased tissue NO concentrations by 85% in the renal cortex and 150% in the renal medulla. Renal medullary interstitial infusion of L-NAME (1.4 µg/kg per minute) reduced medullary NO concentrations and substantially blocked Ang II-induced increases in NO concentrations in the renal medulla, but not in the renal cortex. Tissue slices of the renal cortex and medulla were studied to determine the effects of Ang II and L-NAME on the nitrite/nitrate production. Ang II stimulated the nitrite/nitrate production predominately in the renal medulla, which was significantly attenuated by L-NAME. We conclude that small elevations of circulating Ang II levels increase medullary NO production and concentrations, which plays an important role in buffering the vasoconstrictor effects of this peptide and in maintaining a constancy of MBF.
Key Words: nitric oxide • angiotensin II • renal hemodynamics • renal medulla • laser Doppler flowmetry
Abbreviations: Ang II = angiotensin II • CBF = cortical blood flow • HETE = hydroxyeicosatetraenoic acid • L-NAME = No-nitro-L-arginine methyl ester • MBF = medullary blood flow • MAP = mean arterial pressure • NO = nitric oxide • NOS = nitric oxide synthase • Po2 = oxygen partial pressure
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