From the Department of Medicine, University of Virginia Health Sciences
Center, Charlottesville, Va.
Correspondence to Robert M. Carey, MD, Box 395, University of Virginia Health Sciences Center, Charlottesville, VA 22908. E-mail RMC4C{at}virginia.edu
Abstract—Angiotensin
II exerts its effects on cardiovascular function and
water and sodium homeostasis by interacting with plasma membrane
receptors on target organs. The existence of subtype 2
angiotensin II (AT2) receptors in the rat heart
has been demonstrated by ligand binding and reverse
transcription–polymerase chain reaction. In the present study, the
expression and localization of AT2 receptor protein in the
rat heart was investigated using an antipeptide polyclonal antibody
against the native rat AT2 receptor by light microscopic
immunocytochemistry and Western blot analysis. In frozen tissue
sections, positive immunostaining was observed in the
myocardium and coronary vessels throughout the
ventricle and atrium of neonatal and young rat hearts. Coronary
vessels of the neonatal heart were more intensely stained compared with
the surrounding myocardium. Positive immunoreactivity in
the coronary vessels of young rats was localized to vascular
endothelium but not in the smooth muscle cells.
Preadsorption controls were all negative. Western blot analysis
showed that the AT2 receptor protein (
© 1998 American Heart Association, Inc.
Scientific Contributions
Immunolocalization of Subtype 2 Angiotensin II (AT2) Receptor Protein in Rat Heart
44 kDa) was
detectable from the AT2 receptor–transfected COS-7 cells
and neonatal rat cardiac myocytes but not from fibroblasts or young rat
aortic smooth muscle cells. The neonatal rat heart expressed
significantly more AT2 receptors than young rat heart.
These data provide the first direct evidence for the expression and
localization of AT2 receptor protein in the rat heart.
Key Words: receptors, angiotensin II • heart • immunocytochemistry • myocardium • rats
This article has been cited by other articles:
 |
|
 |
|
  A. K. Stennett, X. Qiao, A. E. Falone, V. V. Koledova, and R. A. Khalil Increased vascular angiotensin type 2 receptor expression and NOS-mediated mechanisms of vascular relaxation in pregnant rats Am J Physiol Heart Circ Physiol, March 1, 2009; 296(3): H745 - H755. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Savoia, R. M. Touyz, M. Volpe, and E. L. Schiffrin Angiotensin Type 2 Receptor in Resistance Arteries of Type 2 Diabetic Hypertensive Patients Hypertension, February 1, 2007; 49(2): 341 - 346. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Maitland, L. Bridges, W. P. Davis, J. Loscalzo, and M. A. Pointer Different Effects of Angiotensin Receptor Blockade on End-Organ Damage in Salt-Dependent and Salt-Independent Hypertension Circulation, August 29, 2006; 114(9): 905 - 911. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Abadir, A. Periasamy, R. M. Carey, and H. M. Siragy Angiotensin II Type 2 Receptor-Bradykinin B2 Receptor Functional Heterodimerization Hypertension, August 1, 2006; 48(2): 316 - 322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nakayama, X. Yan, R. L. Price, T. K. Borg, K. Ito, A. Sanbe, J. Robbins, and B. H. Lorell Chronic ventricular myocyte-specific overexpression of angiotensin II type 2 receptor results in intrinsic myocyte contractile dysfunction Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H317 - H327. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. M. Siragy, C. Xue, P. Abadir, and R. M. Carey Angiotensin Subtype-2 Receptors Inhibit Renin Biosynthesis and Angiotensin II Formation Hypertension, January 1, 2005; 45(1): 133 - 137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Kumar, V. Menon, W. R. Ford, A. S. Clanachan, and B. I. Jugdutt Effect of Angiotensin II lype 2 Receptor Blockade on Activation of Mitogen-Activated Protein Kinases after Ischemia-Reperfusion in Isolated Working Rat Hearts Journal of Cardiovascular Pharmacology and Therapeutics, December 1, 2003; 8(4): 285 - 296. [Abstract] [PDF]
|
|
|
|
|
|
X. Yan, R. L. Price, M. Nakayama, K. Ito, A. J. T. Schuldt, W. J. Manning, A. Sanbe, T. K. Borg, J. Robbins, and B. H. Lorell Ventricular-specific expression of angiotensin II type 2 receptors causes dilated cardiomyopathy and heart failure in transgenic mice Am J Physiol Heart Circ Physiol, November 1, 2003; 285(5): H2179 - H2187. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Carey and H. M. Siragy Newly Recognized Components of the Renin-Angiotensin System: Potential Roles in Cardiovascular and Renal Regulation Endocr. Rev., June 1, 2003; 24(3): 261 - 271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kurisu, R. Ozono, T. Oshima, M. Kambe, T. Ishida, H. Sugino, H. Matsuura, K. Chayama, Y. Teranishi, O. Iba, et al. Cardiac Angiotensin II Type 2 Receptor Activates the Kinin/NO System and Inhibits Fibrosis Hypertension, January 1, 2003; 41(1): 99 - 107. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Cao, F. Bonnet, R. Candido, S. P. Nesteroff, W. C. Burns, H. Kawachi, F. Shimizu, R. M. Carey, M. de Gasparo, and M. E. Cooper Angiotensin Type 2 Receptor Antagonism Confers Renal Protection in a Rat Model of Progressive Renal Injury J. Am. Soc. Nephrol., July 1, 2002; 13(7): 1773 - 1787. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xu, D. Kumar, J. R. B. Dyck, W. R. Ford, A. S. Clanachan, G. D. Lopaschuk, and B. I. Jugdutt AT1 and AT2 receptor expression and blockade after acute ischemia-reperfusion in isolated working rat hearts Am J Physiol Heart Circ Physiol, April 1, 2002; 282(4): H1206 - H1215. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Carey, N. L. Howell, X.-H. Jin, and H. M. Siragy Angiotensin Type 2 Receptor-Mediated Hypotension in Angiotensin Type-1 Receptor-Blocked Rats Hypertension, December 1, 2001; 38(6): 1272 - 1277. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Deinum, J. M.G. van Gool, M. J.M. Kofflard, F. J. ten Cate, and A.H. J. Danser Angiotensin II Type 2 Receptors and Cardiac Hypertrophy in Women With Hypertrophic Cardiomyopathy Hypertension, December 1, 2001; 38(6): 1278 - 1281. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Sugawara, K. Takeuchi, A. Uruno, Y. Ikeda, S. Arima, M. Kudo, K. Sato, Y. Taniyama, and S. Ito Transcriptional Suppression of Type 1 Angiotensin II Receptor Gene Expression by Peroxisome Proliferator-Activated Receptor-{{gamma}} in Vascular Smooth Muscle Cells Endocrinology, July 1, 2001; 142(7): 3125 - 3134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Varagic, D. Susic, and E. D. Frohlich Coronary Hemodynamic and Ventricular Responses to Angiotensin Type 1 Receptor Inhibition in SHR : Interaction With Angiotensin Type 2 Receptors Hypertension, June 1, 2001; 37(6): 1399 - 1403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. F. Moore, N. T. Heiderstadt, E. Huang, N. L. Howell, Z.-Q. Wang, H. M. Siragy, and R. M. Carey Selective Inhibition of the Renal Angiotensin Type 2 Receptor Increases Blood Pressure in Conscious Rats Hypertension, May 1, 2001; 37(5): 1285 - 1291. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Barlucchi, A. Leri, D. E. Dostal, F. Fiordaliso, H. Tada, T. H. Hintze, J. Kajstura, B. Nadal-Ginard, and P. Anversa Canine Ventricular Myocytes Possess a Renin-Angiotensin System That Is Upregulated With Heart Failure Circ. Res., February 16, 2001; 88(3): 298 - 304. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J. Wehbi, J. Zimpelmann, R. M. Carey, D. Z. Levine, and K. D. Burns Early streptozotocin-diabetes mellitus downregulates rat kidney AT2 receptors Am J Physiol Renal Physiol, February 1, 2001; 280(2): F254 - F265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Akishita, M. Iwai, L. Wu, L. Zhang, Y. Ouchi, V. J. Dzau, and M. Horiuchi Inhibitory Effect of Angiotensin II Type 2 Receptor on Coronary Arterial Remodeling After Aortic Banding in Mice Circulation, October 3, 2000; 102(14): 1684 - 1689. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Busche, S. Gallinat, R.-M. Bohle, A. Reinecke, J. Seebeck, F. Franke, L. Fink, M. Zhu, C. Sumners, and T. Unger Expression of Angiotensin AT1 and AT2 Receptors in Adult Rat Cardiomyocytes after Myocardial Infarction : A Single-Cell Reverse Transcriptase-Polymerase Chain Reaction Study Am. J. Pathol., August 1, 2000; 157(2): 605 - 611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Yi Xu, V. Menon, and B. I Jugdutt Cardioprotection after angiotensin II type 1 blockade involves angiotensin II type 2 receptor expression and activation of protein kinase C-{varepsilon} in acutely reperfused myocardial infarction in the dog: Effect of UP269-6 and losartan on AT1- and AT2-receptor expression and IP3 receptor and PKC{varepsilon} proteins Journal of Renin-Angiotensin-Aldosterone System, June 1, 2000; 1(2): 184 - 195. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Gallinat, S. Busche, M. K. Raizada, and C. Sumners The angiotensin II type 2 receptor: an enigma with multiple variations Am J Physiol Endocrinol Metab, March 1, 2000; 278(3): E357 - E374. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Ozono, T. Matsumoto, T. Shingu, T. Oshima, Y. Teranishi, M. Kambe, H. Matsuura, G. Kajiyama, Z.-Q. Wang, A. F. Moore, et al. Expression and localization of angiotensin subtype receptor proteins in the hypertensive rat heart Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2000; 278(3): R781 - R789. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Carey, Z.-Q. Wang, and H. M. Siragy Role of the Angiotensin Type 2 Receptor in the Regulation of Blood Pressure and Renal Function Hypertension, January 1, 2000; 35(1): 155 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gunasegaram, R. S. Haworth, D. J. Hearse, and M. Avkiran Regulation of Sarcolemmal Na+/H+ Exchanger Activity by Angiotensin II in Adult Rat Ventricular Myocytes : Opposing Actions via AT1 Versus AT2 Receptors Circ. Res., November 12, 1999; 85(10): 919 - 930. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. C Wollert and H. Drexler The renin-angiotensin system and experimental heart failure Cardiovasc Res, September 1, 1999; 43(4): 838 - 849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-Q. Wang, L. J. Millatt, N. T. Heiderstadt, H. M. Siragy, R. A. Johns, and R. M. Carey Differential Regulation of Renal Angiotensin Subtype AT1A and AT2 Receptor Protein in Rats With Angiotensin-Dependent Hypertension Hypertension, January 1, 1999; 33(1): 96 - 101. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Touyz, D. Endemann, G. He, J.-S. Li, and E. L. Schiffrin Role of AT2 Receptors in Angiotensin II–Stimulated Contraction of Small Mesenteric Arteries in Young SHR Hypertension, January 1, 1999; 33(1): 366 - 372. [Abstract] [Full Text] [PDF]
|
|