From the Department of Physiology, Tulane University School of Medicine,
New Orleans, La.
Correspondence to Dewan S.A. Majid, PhD, Department of Physiology SL39, Tulane University School of Medicine, 1430 Tulane Ave, New Orleans, La 70112. E-mail majid{at}mailhost.tcs.tulane.edu
Abstract—Recent studies have
indicated that changes in intrarenal nitric oxide (NO)
production participate in mediating arterial
pressure–induced changes in urinary sodium excretion. Until recently,
however, the means to measure changes in intrarenal NO activity in vivo
have not been available. For the present study, changes in renal
tissue NO activities were assessed directly using an NO-selective
microelectrode inserted into the cortical tissue of
anesthetized dogs. Control studies demonstrated that the
electrode was responsive to intrarenal bolus injections of
acetylcholine and to the NO donor
S-nitroso-acetylpenicillamine (SNAP). Intrarenal
nitro-L-arginine (50 µg · kg-1
· min-1) decreased renal tissue NO concentration by
593±127 nmol/L (P<0.05; n=7). Infusions of SNAP (1, 2,
and 3 µg · kg-1 · min-1 for
25 minutes) in nitro-L-arginine–treated dogs (n=5)
resulted in dose-dependent increases in renal tissue NO activity, which
showed a positive correlation with changes in urinary excretion rates
of NO metabolites, nitrates and nitrites, (r=0.62,
P<0.05) and sodium (r=0.78,
P<0.01). During graded reductions of renal
arterial pressure within the autoregulatory range (144±3
to 73±2 mm Hg; n=10), there were decreases in tissue NO activity
that were positively correlated with changes in renal
arterial pressure (r=0.45;
P<0.05), urinary nitrate/nitrite excretion
(r=0.64, P<0.005), and urinary sodium
excretion (r=0.46; P<0.05). These data
support the hypothesis that acute changes in renal arterial
pressure result in alterations in intrarenal NO activity, which may be
responsible for the associated changes in sodium excretion.
© 1998 American Heart Association, Inc.
Scientific Contributions
Intrarenal Nitric Oxide Activity and Pressure Natriuresis in Anesthetized Dogs
Key Words: nitric oxide electrode • sodium excretion • nitrate excretion • pressure-diuresis
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