From the Department of Medicine, Division of Cardiology, Emory
University, Atlanta, Ga.
Correspondence to Kathy K. Griendling, PhD, Division of Cardiology, Emory University School of Medicine, 1639 Pierce Dr, Room 319, Atlanta, GA 30322. E-mail kgriend{at}emory.edu
Abstract—Recent evidence
suggests that oxidative mechanisms may be involved in vascular smooth
muscle cell (VSMC) hypertrophy. We previously showed that
angiotensin II (Ang II) increases superoxide
production by activating an NADH/NADPH oxidase, which
contributes to hypertrophy. In this study, we determined
whether Ang II stimulation of this oxidase results in
H2O2 production by studying the effects
of Ang II on intracellular H2O2 generation,
intracellular superoxide dismutase and catalase activity, and
hypertrophy. Ang II (100 nmol/L) significantly increased
intracellular H2O2 levels at 4 hours. Neither
superoxide dismutase activity nor catalase activity was affected by Ang
II; the SOD present in VSMCs is sufficient to metabolize Ang
II–stimulated superoxide to H2O2, which
accumulates more rapidly than it is degraded by catalase. This increase
in H2O2 was inhibited by extracellular
catalase, diphenylene iodonium, an inhibitor of the
NADH/NADPH oxidase, and the AT1 receptor blocker
losartan. In VSMCs stably transfected with antisense p22phox, a
critical component of the NADH/NADPH oxidase in which oxidase activity
was markedly reduced, Ang II–induced production of
H2O2 was almost completely inhibited,
confirming that the source of Ang II–induced
H2O2 was the NADH/NADPH oxidase. Using a novel
cell line that stably overexpresses catalase, we showed that this
increased H2O2 is a critical step in VSMC
hypertrophy, a hallmark of many vascular diseases.
Inhibition of intracellular superoxide dismutase by
diethylthiocarbamate (1 mmol/L) also resulted in attenuation of
Ang II–induced hypertrophy (62±2% inhibition). These
data indicate that AT1 receptor–mediated
production of superoxide generated by the NADH/NADPH oxidase is
followed by an increase in intracellular H2O2,
suggesting a specific role for these oxygen species and scavenging
systems in modifying the intracellular redox state in vascular
growth.
© 1998 American Heart Association, Inc.
Scientific Contributions
Role of NADH/NADPH Oxidase–Derived H2O2 in Angiotensin II–Induced Vascular Hypertrophy
Key Words: vascular smooth muscle • angiotensin II • NADH • NADPH oxidase • hydrogen peroxide • superoxide dismutase • catalase • hypertrophy
This article has been cited by other articles:
 |
|
 |
|
  F. Jimenez-Altayo, L. Caracuel, F. J. Perez-Asensio, S. Martinez-Revelles, A. Messeguer, A. M. Planas, and E. Vila Participation of Oxidative Stress on Rat Middle Cerebral Artery Changes Induced by Focal Cerebral Ischemia: Beneficial Effects of 3,4-Dihydro-6-hydroxy-7-methoxy-2,2-dimethyl-1(2H)-benzopyran (CR-6) J. Pharmacol. Exp. Ther., November 1, 2009; 331(2): 429 - 436. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Y. Jin, J. L. Sartoretto, V. N. Gladyshev, and T. Michel Endothelial nitric oxide synthase negatively regulates hydrogen peroxide-stimulated AMP-activated protein kinase in endothelial cells PNAS, October 13, 2009; 106(41): 17343 - 17348. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Wang and M. F. Lou The Regulation of NADPH Oxidase and Its Association with Cell Proliferation in Human Lens Epithelial Cells Invest. Ophthalmol. Vis. Sci., May 1, 2009; 50(5): 2291 - 2300. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Yamaguchi, T. Kaneshiro, S.-i. Saitoh, T. Ishibashi, Y. Maruyama, and Y. Takeishi Regulation of coronary vascular tone via redox modulation in the {alpha}1-adrenergic-angiotensin-endothelin axis of the myocardium Am J Physiol Heart Circ Physiol, January 1, 2009; 296(1): H226 - H232. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Li, X. Dai, S. Watts, D. Kreulen, and G. Fink Increased superoxide levels in ganglia and sympathoexcitation are involved in sarafotoxin 6c-induced hypertension Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2008; 295(5): R1546 - R1554. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Zhang, P. Malik, D. Pandey, S. Gupta, D. Jagnandan, E. B. de Chantemele, B. Banfi, M. B. Marrero, R. D. Rudic, D. W. Stepp, et al. Paradoxical Activation of Endothelial Nitric Oxide Synthase by NADPH Oxidase Arterioscler Thromb Vasc Biol, September 1, 2008; 28(9): 1627 - 1633. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. C Parish and J. D Evans Inflammation in Chronic Heart Failure Ann. Pharmacother., July 1, 2008; 42(7): 1002 - 1016. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. R. Kline, D. J. Kleinhenz, B. Liang, S. Dikalov, D. M. Guidot, C. M. Hart, D. P. Jones, and R. L. Sutliff Vascular oxidative stress and nitric oxide depletion in HIV-1 transgenic rats are reversed by glutathione restoration Am J Physiol Heart Circ Physiol, June 1, 2008; 294(6): H2792 - H2804. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Feihl, L. Liaudet, B. I. Levy, and B. Waeber Hypertension and microvascular remodelling Cardiovasc Res, May 1, 2008; 78(2): 274 - 285. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. E. Herbert, Y. Mistry, R. Hastings, T. Poolman, L. Niklason, and B. Williams Angiotensin II-Mediated Oxidative DNA Damage Accelerates Cellular Senescence in Cultured Human Vascular Smooth Muscle Cells via Telomere-Dependent and Independent Pathways Circ. Res., February 1, 2008; 102(2): 201 - 208. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Weiss and W. R. Taylor Deoxycorticosterone Acetate Salt Hypertension in Apolipoprotein E-/- Mice Results in Accelerated Atherosclerosis: The Role of Angiotensin II Hypertension, February 1, 2008; 51(2): 218 - 224. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.-X. Zhang, X.-M. Lu, S. Kimura, and A. Nishiyama Role of mitochondria in angiotensin II-induced reactive oxygen species and mitogen-activated protein kinase activation Cardiovasc Res, November 1, 2007; 76(2): 204 - 212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Chen, A. Pearlman, Z. Luo, and C. S. Wilcox Hydrogen peroxide mediates a transient vasorelaxation with tempol during oxidative stress Am J Physiol Heart Circ Physiol, October 1, 2007; 293(4): H2085 - H2092. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Sachse and G. Wolf Angiotensin II Induced Reactive Oxygen Species and the Kidney J. Am. Soc. Nephrol., September 1, 2007; 18(9): 2439 - 2446. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Haurani and P. J. Pagano Adventitial fibroblast reactive oxygen species as autacrine and paracrine mediators of remodeling: Bellwether for vascular disease? Cardiovasc Res, September 1, 2007; 75(4): 679 - 689. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. C. Tu, H. Sun, G. T. Bowden, and Q. M. Chen Involvement of oxidants and AP-1 in angiotensin II-activated NFAT3 transcription factor Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1248 - C1255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. K. Mehta and K. K. Griendling Angiotensin II cell signaling: physiological and pathological effects in the cardiovascular system Am J Physiol Cell Physiol, January 1, 2007; 292(1): C82 - C97. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D. Hingtgen, X. Tian, J. Yang, S. M. Dunlay, A. S. Peek, Y. Wu, R. V. Sharma, J. F. Engelhardt, and R. L. Davisson Nox2-containing NADPH oxidase and Akt activation play a key role in angiotensin II-induced cardiomyocyte hypertrophy Physiol Genomics, September 14, 2006; 26(3): 180 - 191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. N. Lyle and K. K. Griendling Modulation of vascular smooth muscle signaling by reactive oxygen species. Physiology, August 1, 2006; 21: 269 - 280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Suzuki, M. Iwai, M. Mogi, A. Oshita, T. Yoshii, J. Higaki, and M. Horiuchi Eplerenone With Valsartan Effectively Reduces Atherosclerotic Lesion by Attenuation of Oxidative Stress and Inflammation Arterioscler Thromb Vasc Biol, April 1, 2006; 26(4): 917 - 921. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Menshikov, O. Plekhanova, H. Cai, K. Chalupsky, Y. Parfyonova, P. Bashtrikov, V. Tkachuk, and B. C. Berk Urokinase Plasminogen Activator Stimulates Vascular Smooth Muscle Cell Proliferation Via Redox-Dependent Pathways Arterioscler Thromb Vasc Biol, April 1, 2006; 26(4): 801 - 807. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Ardanaz and P. J. Pagano Hydrogen peroxide as a paracrine vascular mediator: regulation and signaling leading to dysfunction. Experimental Biology and Medicine, March 1, 2006; 231(3): 237 - 251. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Polikandriotis, H. L. Rupnow, S. C. Elms, R. E. Clempus, D. J. Campbell, R. L. Sutliff, L. A. S. Brown, D. M. Guidot, and C. M. Hart Chronic Ethanol Ingestion Increases Superoxide Production and NADPH Oxidase Expression in the Lung Am. J. Respir. Cell Mol. Biol., March 1, 2006; 34(3): 314 - 319. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Fortuno, G. San Jose, M. U. Moreno, O. Beloqui, J. Diez, and G. Zalba Phagocytic NADPH Oxidase Overactivity Underlies Oxidative Stress in Metabolic Syndrome Diabetes, January 1, 2006; 55(1): 209 - 215. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ishizaka, K. Saito, I. Mori, G. Matsuzaki, M. Ohno, and R. Nagai Iron Chelation Suppresses Ferritin Upregulation and Attenuates Vascular Dysfunction in the Aorta of Angiotensin II-Infused Rats Arterioscler Thromb Vasc Biol, November 1, 2005; 25(11): 2282 - 2288. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. F. Keaney Jr Oxidative Stress and the Vascular Wall: NADPH Oxidases Take Center Stage Circulation, October 25, 2005; 112(17): 2585 - 2588. [Full Text] [PDF]
|
|
|
|
|
|
A. Dikalova, R. Clempus, B. Lassegue, G. Cheng, J. McCoy, S. Dikalov, A. S. Martin, A. Lyle, D. S. Weber, D. Weiss, et al. Nox1 Overexpression Potentiates Angiotensin II-Induced Hypertension and Vascular Smooth Muscle Hypertrophy in Transgenic Mice Circulation, October 25, 2005; 112(17): 2668 - 2676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Wilcox Oxidative stress and nitric oxide deficiency in the kidney: a critical link to hypertension? Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2005; 289(4): R913 - R935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Cai Hydrogen peroxide regulation of endothelial function: Origins, mechanisms, and consequences Cardiovasc Res, October 1, 2005; 68(1): 26 - 36. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhang, K. K. Griendling, A. Dikalova, G. K. Owens, and W. R. Taylor Vascular Hypertrophy in Angiotensin II-Induced Hypertension Is Mediated by Vascular Smooth Muscle Cell-Derived H2O2 Hypertension, October 1, 2005; 46(4): 732 - 737. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Iwai, R. Chen, Z. Li, T. Shiuchi, J. Suzuki, A. Ide, M. Tsuda, M. Okumura, L.-J. Min, M. Mogi, et al. Deletion of Angiotensin II Type 2 Receptor Exaggerated Atherosclerosis in Apolipoprotein E-Null Mice Circulation, September 13, 2005; 112(11): 1636 - 1643. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. I. Bechara, A. Pelaez, A. Palacio, P. C. Joshi, C. M. Hart, L. A. S. Brown, R. Raynor, and D. M. Guidot Angiotensin II mediates glutathione depletion, transforming growth factor-{beta}1 expression, and epithelial barrier dysfunction in the alcoholic rat lung Am J Physiol Lung Cell Mol Physiol, September 1, 2005; 289(3): L363 - L370. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y Shimoni, D Hunt, M Chuang, K. Y Chen, G Kargacin, and D. L Severson Modulation of potassium currents by angiotensin and oxidative stress in cardiac cells from the diabetic rat J. Physiol., August 15, 2005; 567(1): 177 - 190. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-M. Yuan, W.-P. Koh, C.-L. Sun, H.-P. Lee, and M. C. Yu Green tea intake, ACE gene polymorphism and breast cancer risk among Chinese women in Singapore Carcinogenesis, August 1, 2005; 26(8): 1389 - 1394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Gavrila, W. G. Li, M. L. McCormick, M. Thomas, A. Daugherty, L. A. Cassis, F. J. Miller Jr, L. W. Oberley, K. C. Dellsperger, and N. L. Weintraub Vitamin E Inhibits Abdominal Aortic Aneurysm Formation in Angiotensin II-Infused Apolipoprotein E-Deficient Mice Arterioscler Thromb Vasc Biol, August 1, 2005; 25(8): 1671 - 1677. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Ullian, A. K. Gelasco, W. R. Fitzgibbon, C. N. Beck, and T. A. Morinelli N-Acetylcysteine Decreases Angiotensin II Receptor Binding in Vascular Smooth Muscle Cells J. Am. Soc. Nephrol., August 1, 2005; 16(8): 2346 - 2353. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Mata-Greenwood, A. Grobe, S. Kumar, Y. Noskina, and S. M. Black Cyclic stretch increases VEGF expression in pulmonary arterial smooth muscle cells via TGF-{beta}1 and reactive oxygen species: a requirement for NAD(P)H oxidase Am J Physiol Lung Cell Mol Physiol, August 1, 2005; 289(2): L288 - L289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Matsui, S. Xu, K. A. Maitland, A. Hayes, J. A. Leopold, D. E. Handy, J. Loscalzo, and R. A. Cohen Glucose-6 Phosphate Dehydrogenase Deficiency Decreases the Vascular Response to Angiotensin II Circulation, July 12, 2005; 112(2): 257 - 263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Taniyama, H. Hitomi, A. Shah, R. W. Alexander, and K. K. Griendling Mechanisms of Reactive Oxygen Species-Dependent Downregulation of Insulin Receptor Substrate-1 by Angiotensin II Arterioscler Thromb Vasc Biol, June 1, 2005; 25(6): 1142 - 1147. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. R. Madamanchi, S.-K. Moon, Z. S. Hakim, S. Clark, A. Mehrizi, C. Patterson, and M. S. Runge Differential Activation of Mitogenic Signaling Pathways in Aortic Smooth Muscle Cells Deficient in Superoxide Dismutase Isoforms Arterioscler Thromb Vasc Biol, May 1, 2005; 25(5): 950 - 956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Li and K. U. Malik Angiotensin II-induced Akt activation is mediated by metabolites of arachidonic acid generated by CaMKII-stimulated Ca2+-dependent phospholipase A2 Am J Physiol Heart Circ Physiol, May 1, 2005; 288(5): H2306 - H2316. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Cai NAD(P)H Oxidase-Dependent Self-Propagation of Hydrogen Peroxide and Vascular Disease Circ. Res., April 29, 2005; 96(8): 818 - 822. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tsuda, M. Iwai, J.-M. Li, H.-S. Li, L.-J. Min, A. Ide, M. Okumura, J. Suzuki, M. Mogi, H. Suzuki, et al. Inhibitory Effects of AT1 Receptor Blocker, Olmesartan, and Estrogen on Atherosclerosis Via Anti-Oxidative Stress Hypertension, April 1, 2005; 45(4): 545 - 551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R.M. Touyz, G. Yao, M.T. Quinn, P.J. Pagano, and E.L. Schiffrin p47phox Associates With the Cytoskeleton Through Cortactin in Human Vascular Smooth Muscle Cells: Role in NAD(P)H Oxidase Regulation by Angiotensin II Arterioscler Thromb Vasc Biol, March 1, 2005; 25(3): 512 - 518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. A. DeLano, R. Balete, and G. W. Schmid-Schonbein Control of oxidative stress in microcirculation of spontaneously hypertensive rats Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H805 - H812. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H.M. Ellmark, G. J. Dusting, M. Ng Tang Fui, N. Guzzo-Pernell, and G. R. Drummond The contribution of Nox4 to NADPH oxidase activity in mouse vascular smooth muscle Cardiovasc Res, February 1, 2005; 65(2): 495 - 504. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. R. Madamanchi, A. Vendrov, and M. S. Runge Oxidative Stress and Vascular Disease Arterioscler Thromb Vasc Biol, January 1, 2005; 25(1): 29 - 38. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Weber, P. Rocic, A. M. Mellis, K. Laude, A. N. Lyle, D. G. Harrison, and K. K. Griendling Angiotensin II-induced hypertrophy is potentiated in mice overexpressing p22phox in vascular smooth muscle Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H37 - H42. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. J. Villarreal and J. Asbun Peroxisome Proliferator-Activated Receptors Ligands, Oxidative Stress, and Cardiac Fibroblast Extracellular Matrix Turnover Hypertension, November 1, 2004; 44(5): 621 - 622. [Full Text] [PDF]
|
|
|
|
|
|
K. Chen, J. Chen, D. Li, X. Zhang, and J. L. Mehta Angiotensin II Regulation of Collagen Type I Expression in Cardiac Fibroblasts: Modulation by PPAR-{gamma} Ligand Pioglitazone Hypertension, November 1, 2004; 44(5): 655 - 661. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Stocker and J. F. Keaney Jr. Role of Oxidative Modifications in Atherosclerosis Physiol Rev, October 1, 2004; 84(4): 1381 - 1478. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Coronella-Wood, J. Terrand, H. Sun, and Q. M. Chen c-Fos Phosphorylation Induced by H2O2 Prevents Proteasomal Degradation of c-Fos in Cardiomyocytes J. Biol. Chem., August 6, 2004; 279(32): 33567 - 33574. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Wyche, S. S. Wang, K. K. Griendling, S. I. Dikalov, H. Austin, S. Rao, B. Fink, D. G. Harrison, and A. M. Zafari C242T CYBA Polymorphism of the NADPH Oxidase Is Associated With Reduced Respiratory Burst in Human Neutrophils Hypertension, June 1, 2004; 43(6): 1246 - 1251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.-F. Guo, V. Tardif, K. Ghelima, J. S. D. Chan, J. R. Ingelfinger, X. Chen, and I. Chenier A Novel Angiotensin II Type 1 Receptor-associated Protein Induces Cellular Hypertrophy in Rat Vascular Smooth Muscle and Renal Proximal Tubular Cells J. Biol. Chem., May 14, 2004; 279(20): 21109 - 21120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Weber, Y. Taniyama, P. Rocic, P. N. Seshiah, M. A. Dechert, W. T. Gerthoffer, and K. K. Griendling Phosphoinositide-Dependent Kinase 1 and p21-Activated Protein Kinase Mediate Reactive Oxygen Species-Dependent Regulation of Platelet-Derived Growth Factor-Induced Smooth Muscle Cell Migration Circ. Res., May 14, 2004; 94(9): 1219 - 1226. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. Lindley, M. F. Doobay, R. V. Sharma, and R. L. Davisson Superoxide Is Involved in the Central Nervous System Activation and Sympathoexcitation of Myocardial Infarction-Induced Heart Failure Circ. Res., February 20, 2004; 94(3): 402 - 409. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Suzuki, T. Yamamoto, N. Ikegaya, and A. Hishida Dietary salt intake modulates progression of antithymocyte serum nephritis through alteration of glomerular angiotensin II receptor expression Am J Physiol Renal Physiol, February 1, 2004; 286(2): F267 - F277. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Bleeke, H. Zhang, N. Madamanchi, C. Patterson, and J. E. Faber Catecholamine-Induced Vascular Wall Growth Is Dependent on Generation of Reactive Oxygen Species Circ. Res., January 9, 2004; 94(1): 37 - 45. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Taniyama and K. K. Griendling Reactive Oxygen Species in the Vasculature: Molecular and Cellular Mechanisms Hypertension, December 1, 2003; 42(6): 1075 - 1081. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Griendling and G. A. FitzGerald Oxidative Stress and Cardiovascular Injury: Part II: Animal and Human Studies Circulation, October 28, 2003; 108(17): 2034 - 2040. [Full Text] [PDF]
|
|
|
|
|
|
D. Gregg, F. M. Rauscher, and P. J. Goldschmidt-Clermont Rac regulates cardiovascular superoxide through diverse molecular interactions: more than a binary GTP switch Am J Physiol Cell Physiol, October 1, 2003; 285(4): C723 - C734. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kato, H. Yin, J. Agata, H. Yoshida, L. Chao, and J. Chao Adrenomedullin gene delivery attenuates myocardial infarction and apoptosis after ischemia and reperfusion Am J Physiol Heart Circ Physiol, October 1, 2003; 285(4): H1506 - H1514. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Rocic, P. Seshiah, and K. K. Griendling Reactive Oxygen Species Sensitivity of Angiotensin II-dependent Translation Initiation in Vascular Smooth Muscle Cells J. Biol. Chem., September 19, 2003; 278(38): 36973 - 36979. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Lassegue and R. E. Clempus Vascular NAD(P)H oxidases: specific features, expression, and regulation Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2003; 285(2): R277 - R297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Makino, M. M. Skelton, A.-P. Zou, and A. W. Cowley Jr Increased Renal Medullary H2O2 Leads to Hypertension Hypertension, July 1, 2003; 42(1): 25 - 30. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Fries, E. Paxinou, M. Themistocleous, E. Swanberg, K. K. Griendling, D. Salvemini, J. W. Slot, H. F. G. Heijnen, S. L. Hazen, and H. Ischiropoulos Expression of Inducible Nitric-oxide Synthase and Intracellular Protein Tyrosine Nitration in Vascular Smooth Muscle Cells: ROLE OF REACTIVE OXYGEN SPECIES J. Biol. Chem., June 13, 2003; 278(25): 22901 - 22907. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Chiarugi, G. Pani, E. Giannoni, L. Taddei, R. Colavitti, G. Raugei, M. Symons, S. Borrello, T. Galeotti, and G. Ramponi Reactive oxygen species as essential mediators of cell adhesion: the oxidative inhibition of a FAK tyrosine phosphatase is required for cell adhesion J. Cell Biol., June 9, 2003; 161(5): 933 - 944. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G Harrison, Hua Cai, U. Landmesser, and K. K Griendling The Pickering Lecture British Hypertension Society, 10th September 2002: Interactions of angiotensin II with NAD(P)H oxidase, oxidant stress and cardiovascular disease Journal of Renin-Angiotensin-Aldosterone System, June 1, 2003; 4(2): 51 - 61. [Abstract] [PDF]
|
|
|
|
|
|
T. J. Bivalacqua, J. S. Armstrong, J. Biggerstaff, A. B. Abdel-Mageed, P. J. Kadowitz, W. J. G. Hellstrom, and H. C. Champion Gene transfer of extracellular SOD to the penis reduces O Am J Physiol Heart Circ Physiol, April 1, 2003; 284(4): H1408 - H1421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P A J Krijnen, C Meischl, C E Hack, C J L M Meijer, C A Visser, D Roos, and H W M Niessen Increased Nox2 expression in human cardiomyocytes after acute myocardial infarction J. Clin. Pathol., March 1, 2003; 56(3): 194 - 199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Oeckler, P. M. Kaminski, and M. S. Wolin Stretch Enhances Contraction of Bovine Coronary Arteries via an NAD(P)H Oxidase-Mediated Activation of the Extracellular Signal-Regulated Kinase Mitogen-Activated Protein Kinase Cascade Circ. Res., January 10, 2003; 92(1): 23 - 31. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. T. Schumacker Angiotensin II Signaling in the Brain: Compartmentalization of Redox Signaling? Circ. Res., November 29, 2002; 91(11): 982 - 984. [Full Text] [PDF]
|
|
|
|
 |
|
 D. E. Kim, Y. S. Suh, M.-S. Lee, K. Y. Kim, J. H. Lee, H. S. Lee, K. W. Hong, and C. D. Kim Vascular NAD(P)H Oxidase Triggers Delayed Cerebral Vasospasm After Subarachnoid Hemorrhage in Rats Stroke, November 1, 2002; 33(11): 2687 - 2691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Ortiz and J. L. Garvin Superoxide stimulates NaCl absorption by the thick ascending limb Am J Physiol Renal Physiol, November 1, 2002; 283(5): F957 - F962. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. G. B. Haider, D. Sorescu, K. K. Griendling, A. M. Vollmar, and V. M. Dirsch Resveratrol Suppresses Angiotensin II-Induced Akt/Protein Kinase B and p70 S6 Kinase Phosphorylation and Subsequent Hypertrophy in Rat Aortic Smooth Muscle Cells Mol. Pharmacol., October 1, 2002; 62(4): 772 - 777. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. N. Seshiah, D. S. Weber, P. Rocic, L. Valppu, Y. Taniyama, and K. K. Griendling Angiotensin II Stimulation of NAD(P)H Oxidase Activity: Upstream Mediators Circ. Res., September 6, 2002; 91(5): 406 - 413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L Van Heerebeek, C Meischl, W Stooker, C J L M Meijer, H W M Niessen, and D Roos NADPH oxidase(s): new source(s) of reactive oxygen species in the vascular system? J. Clin. Pathol., August 1, 2002; 55(8): 561 - 568. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. NICKENIG, S. BAUDLER, C. MULLER, C. WERNER, N. WERNER, H. WELZEL, K. STREHLOW, and M. BOHM Redox-sensitive vascular smooth muscle cell proliferation is mediated by GKLF and Id3 in vitro and in vivo FASEB J, July 1, 2002; 16(9): 1077 - 1086. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Mueller, S. Baudler, H. Welzel, M. Bohm, and G. Nickenig Identification of a Novel Redox-Sensitive Gene, Id3, Which Mediates Angiotensin II-Induced Cell Growth Circulation, May 21, 2002; 105(20): 2423 - 2428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kumaran and K. Shivakumar Calcium- and superoxide anion-mediated mitogenic action of substance P on cardiac fibroblasts Am J Physiol Heart Circ Physiol, May 1, 2002; 282(5): H1855 - H1862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nishida, K. L. Schey, S. Takagahara, K. Kontani, T. Katada, Y. Urano, T. Nagano, T. Nagao, and H. Kurose Activation Mechanism of Gi and Go by Reactive Oxygen Species J. Biol. Chem., March 8, 2002; 277(11): 9036 - 9042. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. JAVESGHANI, S. A. MAGDER, E. BARREIRO, M. T. QUINN, and S. N. A. HUSSAIN Molecular Characterization of a Superoxide-Generating NAD(P)H Oxidase in the Ventilatory Muscles Am. J. Respir. Crit. Care Med., February 1, 2002; 165(3): 412 - 418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. K. Kim, M.-S. Lee, S. M. Son, I. J. Kim, W. S. Lee, B. Y. Rhim, K. W. Hong, and C. D. Kim Vascular NADH Oxidase Is Involved in Impaired Endothelium-Dependent Vasodilation in OLETF Rats, a Model of Type 2 Diabetes Diabetes, February 1, 2002; 51(2): 522 - 527. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hirotani, K. Otsu, K. Nishida, Y. Higuchi, T. Morita, H. Nakayama, O. Yamaguchi, T. Mano, Y. Matsumura, H. Ueno, et al. Involvement of Nuclear Factor-{kappa}B and Apoptosis Signal-Regulating Kinase 1 in G-Protein-Coupled Receptor Agonist-Induced Cardiomyocyte Hypertrophy Circulation, January 29, 2002; 105(4): 509 - 515. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. El Hadri, M. Moldes, N. Mercier, M. Andreani, J. Pairault, and B. Feve Semicarbazide-Sensitive Amine Oxidase in Vascular Smooth Muscle Cells: Differentiation-Dependent Expression and Role in Glucose Uptake Arterioscler Thromb Vasc Biol, January 1, 2002; 22(1): 89 - 94. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Droge Free Radicals in the Physiological Control of Cell Function Physiol Rev, January 1, 2002; 82(1): 47 - 95. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Uhlenius, O. Vuolteenaho, and I. Tikkanen Renin-angiotensin blockade improves renal cGMP production via non-AT2-receptor mediated mechanisms in hypertension-induced by chronic NOS inhibition in rat Journal of Renin-Angiotensin-Aldosterone System, December 1, 2001; 2(4): 233 - 239. [Abstract] [PDF]
|
|
|
|
|
|
C. Berry, R. Touyz, A. F. Dominiczak, R. C. Webb, and D. G. Johns Angiotensin receptors: signaling, vascular pathophysiology, and interactions with ceramide Am J Physiol Heart Circ Physiol, December 1, 2001; 281(6): H2337 - H2365. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Zalba, G. S. Jose, M. U. Moreno, M. A. Fortuno, A. Fortuno, F. J. Beaumont, and J. Diez Oxidative Stress in Arterial Hypertension: Role of NAD(P)H Oxidase Hypertension, December 1, 2001; 38(6): 1395 - 1399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Beswick, A. M. Dorrance, R. Leite, and R. C. Webb NADH/NADPH Oxidase and Enhanced Superoxide Production in the Mineralocorticoid Hypertensive Rat Hypertension, November 1, 2001; 38(5): 1107 - 1111. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Silacci, A. Desgeorges, L. Mazzolai, C. Chambaz, and D. Hayoz Flow Pulsatility Is a Critical Determinant of Oxidative Stress in Endothelial Cells Hypertension, November 1, 2001; 38(5): 1162 - 1166. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Jaimes, C. Sweeney, and L. Raij Effects of the Reactive Oxygen Species Hydrogen Peroxide and Hypochlorite on Endothelial Nitric Oxide Production Hypertension, October 1, 2001; 38(4): 877 - 883. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Munzel and J. F. Keaney Jr Are ACE Inhibitors a "Magic Bullet" Against Oxidative Stress? Circulation, September 25, 2001; 104(13): 1571 - 1574. [Full Text] [PDF]
|
|
|
|
|
|
S. L. Lee, A. R. Simon, W. W. Wang, and B. L. Fanburg H2O2 signals 5-HT-induced ERK MAP kinase activation and mitogenesis of smooth muscle cells Am J Physiol Lung Cell Mol Physiol, September 1, 2001; 281(3): L646 - L652. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. C. Berk Vascular Smooth Muscle Growth: Autocrine Growth Mechanisms Physiol Rev, July 1, 2001; 81(3): 999 - 1030. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. L Reeve, S. Tolarova, D. P Nelson, S. Archer, and E K. Weir Redox control of oxygen sensing in the rabbit ductus arteriosus J. Physiol., May 15, 2001; 533(1): 253 - 261. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Irani Angiotensin II-Stimulated Vascular Remodeling : The Search for the Culprit Oxidase Circ. Res., May 11, 2001; 88(9): 858 - 860. [Full Text] [PDF]
|
|
|
|
|
|
Y. Shi, R. Niculescu, D. Wang, S. Patel, K. L. Davenpeck, and A. Zalewski Increased NAD(P)H Oxidase and Reactive Oxygen Species in Coronary Arteries After Balloon Injury Arterioscler Thromb Vasc Biol, May 1, 2001; 21(5): 739 - 745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Stouffer, C. Patterson, N. Madamanchi, and M. S. Runge Role of Reactive Oxygen Species in Angiotensin II Signaling : The Plot Thickens Arterioscler Thromb Vasc Biol, April 1, 2001; 21(4): 471 - 472. [Full Text] [PDF]
|
|