From the Department of Physiology and Biophysics, University of
Mississippi Medical Center, Jackson, Miss.
Correspondence to Michael W. Brands, PhD, Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, MS 39216. E-mail mbrands{at}physiology.umsmed.edu
Abstract—Vascular injury and
impaired vascular function are central to the increased mortality
associated with diabetes. Hyperglycemia in diabetes has been suggested
to play a role in this process, in part by impairing the function of
the vascular endothelium. It has been difficult,
however, to isolate the direct effect of glucose in both humans and in
animal models of diabetes. This was evaluated in the present study
in 7 rats that were chronically instrumented with a Transonic flow
probe at the iliac bifurcation of the abdominal aorta, a nonoccluding
catheter inserted immediately anterior to the flow probe, and a femoral
vein catheter. Acute infusions of acetylcholine and sodium
nitroprusside (1 and 10 µg/min IA) increased hindquarter blood flow
significantly by approximately 27 and 10 mL/min over baseline,
respectively, at the high dose. Streptozotocin (70 mg/kg IV) was
administered, but normoglycemia was maintained with continuous
intravenous insulin infusion to control for potential
streptozotocin side effects. Diabetes was induced 5 to 7 days later by
stopping the insulin infusion. Hindlimb blood flow (measured 24 hours
per day) decreased during the diabetic period and was accompanied by an
increase in mean arterial pressure, suggesting a
vasoconstrictor response. However, the responses to acetylcholine and
sodium nitroprusside were not altered significantly on either day 2 or
day 6 of the diabetic period. This suggests that neither
endothelium-mediated vasorelaxation nor responsiveness
to nitric oxide is impaired during the initial phase of diabetes and
that diabetic hyperglycemia does not have a significant, direct effect
to impair endothelium-mediated relaxation in
insulin-dependent diabetes mellitus. The mechanism for the change in
baseline blood flow and its potential influence on
endothelial function, however, are not known.
© 1998 American Heart Association, Inc.
Scientific Contributions
Acute Endothelium-Mediated Vasodilation Is Not Impaired at the Onset of Diabetes
Key Words: endothelium-derived relaxing factor • blood flow • diabetes • glucose • vasodilation • nitric oxide
This article has been cited by other articles:
 |
|
 |
|
  T. D. Bell, G. F. DiBona, R. Biemiller, and M. W. Brands Continuously measured renal blood flow does not increase in diabetes if nitric oxide synthesis is blocked Am J Physiol Renal Physiol, November 1, 2008; 295(5): F1449 - F1456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. D. Bell, G. F. DiBona, Y. Wang, and M. W. Brands Mechanisms for Renal Blood Flow Control Early in Diabetes as Revealed by Chronic Flow Measurement and Transfer Function Analysis J. Am. Soc. Nephrol., August 1, 2006; 17(8): 2184 - 2192. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Ladeia, C. Ladeia-Frota, L. Pinho, E. Stefanelli, and L. Adan Endothelial Dysfunction Is Correlated With Microalbuminuria in Children With Short-Duration Type 1 Diabetes Diabetes Care, August 1, 2005; 28(8): 2048 - 2050. [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kobayashi, T. Matsumoto, K. Ooishi, and K. Kamata Differential expression of {alpha}2D-adrenoceptor and eNOS in aortas from early and later stages of diabetes in Goto-Kakizaki rats Am J Physiol Heart Circ Physiol, July 1, 2004; 287(1): H135 - H148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Sigaudo-Roussel, C. Demiot, B. Fromy, A. Koitka, G. Leftheriotis, P. Abraham, and J. L. Saumet Early Endothelial Dysfunction Severely Impairs Skin Blood Flow Response to Local Pressure Application in Streptozotocin-Induced Diabetic Mice Diabetes, June 1, 2004; 53(6): 1564 - 1569. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. W. Brands, T. D. Bell, and B. Gibson Nitric Oxide May Prevent Hypertension Early in Diabetes by Counteracting Renal Actions of Superoxide Hypertension, January 1, 2004; 43(1): 57 - 63. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Wendler, P. Landwehr, D. Bandner-Risch, T. Georg, and H.-J. Schafers Vasoreactivity of arterial grafts in the patient with diabetes mellitus: investigations on internal thoracic artery and radial artery conduits Eur. J. Cardiothorac. Surg., August 1, 2001; 20(2): 305 - 311. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Calles-Escandon and M. Cipolla Diabetes and Endothelial Dysfunction: A Clinical Perspective Endocr. Rev., February 1, 2001; 22(1): 36 - 52. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. M. Fitzgerald and M. W. Brands Nitric oxide may be required to prevent hypertension at the onset of diabetes Am J Physiol Endocrinol Metab, October 1, 2000; 279(4): E762 - E768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. Brands, S. M. Fitzgerald, W. H. Hewitt, and A. E. Hailman Decreased cardiac output at the onset of diabetes: renal mechanisms and peripheral vasoconstriction Am J Physiol Endocrinol Metab, May 1, 2000; 278(5): E917 - E924. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Fitzgerald and M. W. Brands Hypertension in L-NAME-treated diabetic rats depends on an intact sympathetic nervous system Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2002; 282(4): R1070 - R1076. [Abstract] [Full Text] [PDF]
|
|