From the 1st Department of Internal Medicine, Shiga University of Medical
Science, Otsu, Japan.
Correspondence to Naoharu Iwai, MD, 1st Department of Internal Medicine, Shiga University of Medical Science, Otsu, Japan. E-mail iwai{at}suncuore.shiga-med.ac.jp
Abstract—Linkage
analyses in the spontaneously hypertensive rat (SHR) suggest
that a gene involved in blood pressure regulation may be located on rat
chromosome 1, in the Sa region. To confirm this
possibility, we replaced a region of chromosome 1 in the Wistar-Kyoto
rat (WKY) defined by the markers D1Mit3 and
MTPA with the corresponding chromosome segment from SHR.
Genotyping using 65 polymorphic microsatellite markers throughout
the entire genome confirmed the congenic status of this new strain
designated WKY.SHR-D1Mit3/Rat57. In male WKY.SHR-D1Mit3/Rat57, mean
blood pressures in the daytime and in the nighttime assessed by
radiotelemetry were significantly higher than those in male progenitor
WKY. Moreover, salt loading significantly increased the mean blood
pressure in male WKY.SHR-D1Mit3/Rat57 but not in male progenitor WKY.
The present study confirmed the existence of a gene that
contributes to high blood pressure and salt sensitivity in this
chromosomal segment. This congenic strain represents a new
animal model for fine mapping and characterization of the gene in this
region involved in salt-sensitive hypertension.
© 1998 American Heart Association, Inc.
Scientific Contributions
Isolation of a Chromosome 1 Region That Contributes to High Blood Pressure and Salt Sensitivity
Key Words: blood pressure • rats, inbred SHR • genetics • congenic strain • chromosome 1
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