From the Medical Policlinic/Department of Medicine, Division of
Nephrology, University of Bonn, Germany.
Correspondence to Dirk Bokemeyer, MD, Medizinische Poliklinik, University of Bonn, Wilhelmstr 35-37, 53111 Bonn, Germany. E-mail bokemeyer{at}uni-bonn.de
Abstract—Mitogen-activated
protein (MAP) kinase cascades are major signaling systems by which
cells transduce extracellular cues into intracellular responses. In
general, MAP kinases are activated by
phosphorylation on tyrosine and threonine residues and
inactivated by dephosphorylation.
Therefore, MAP kinase phosphatase-1 (MKP-1), a dual-specificity protein
tyrosine phosphatase that exhibits catalytic activity toward both
regulatory sites on MAP kinases, is suggested to be responsible for the
downregulation of extracellular signal-regulated kinase (ERK),
stress-activated protein kinase (SAPK), and p38 MAP kinase. In
the present study, we examined the role of these MAP kinases in the
induction of MKP-1 in vascular smooth muscle cells (VSMCs).
Extracellular stimuli such as platelet-derived growth factor
(PDGF), 12-O-tetradecanoylphorbol 13-acetate (TPA),
and angiotensin II, which activated ERK but not
SAPK/p38 MAP kinase, induced a transient induction of MKP-1 mRNA and
its intracellular protein. In addition, PD 098059, an
antagonist of MEK (MAP kinase/ERK kinase), the upstream
kinase of ERK, significantly reduced the PDGF-induced activation of ERK
and potently inhibited the expression of MKP-1 after stimulation with
PDGF, thereby demonstrating the induction of MKP-1 in response to
activation of the ERK signaling cascade. Furthermore, anisomycin, a
potent stimulus of SAPK and p38 MAP kinase, also induced MKP-1 mRNA
expression. This effect of anisomycin was significantly inhibited in
the presence of the p38 MAP kinase antagonist SB 203580.
These data suggest the induction of MKP-1, not only after stimulation
of the cell growth–promoting ERK pathway but also in response to
activation of stress-responsive MAP kinase signaling cascades. We
suggest that this pattern of MKP-1 induction may be a negative feedback
mechanism in the control of MAP kinase activity in VSMCs.
© 1998 American Heart Association, Inc.
Scientific Contributions
Regulation of Mitogen-Activated Protein Kinase Phosphatase-1 in Vascular Smooth Muscle Cells
Key Words: MAP kinase • ERK • SAPK • p38 MAP kinase • MKP-1
This article has been cited by other articles:
 |
|
 |
|
  R. Furst, S. Zahler, and A. M. Vollmar Dexamethasone-Induced Expression of Endothelial Mitogen-Activated Protein Kinase Phosphatase-1 Involves Activation of the Transcription Factors Activator Protein-1 and 3',5'-Cyclic Adenosine 5'-Monophosphate Response Element-Binding Protein and the Generation of Reactive Oxygen Species Endocrinology, July 1, 2008; 149(7): 3635 - 3642. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. K. Mehta and K. K. Griendling Angiotensin II cell signaling: physiological and pathological effects in the cardiovascular system Am J Physiol Cell Physiol, January 1, 2007; 292(1): C82 - C97. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Pulver-Kaste, C. A. Barlow, J. Bond, A. Watson, P. L. Penar, B. Tranmer, and K. M. Lounsbury Ca2+ source-dependent transcription of CRE-containing genes in vascular smooth muscle Am J Physiol Heart Circ Physiol, July 1, 2006; 291(1): H97 - H105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Khan, S. Varadharaj, L. P. Ganesan, J. C. Shobha, M. U. Naidu, N. L. Parinandi, S. Tridandapani, V. K. Kutala, and P. Kuppusamy C-phycocyanin protects against ischemia-reperfusion injury of heart through involvement of p38 MAPK and ERK signaling Am J Physiol Heart Circ Physiol, May 1, 2006; 290(5): H2136 - H2145. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-J. Kim, H.-J. Jeong, I.-Y. Choi, K.-M. Lee, R.-K. Park, S.-H. Hong, and H.-M. Kim Cyclooxygenase-2 Inhibitor SC-236 [4-[5-(4-Chlorophenyl)-3-(trifluoromethyl)-1-pyrazol-1-l] Benzenesulfonamide] Suppresses Nuclear Factor-{kappa}B Activation and Phosphorylation of p38 Mitogen-Activated Protein Kinase, Extracellular Signal-Regulated Kinase, and c-Jun N-Terminal Kinase in Human Mast Cell Line Cells J. Pharmacol. Exp. Ther., July 1, 2005; 314(1): 27 - 34. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Furst, C. Brueckl, W. M. Kuebler, S. Zahler, F. Krotz, A. Gorlach, A. M. Vollmar, and A. K. Kiemer Atrial Natriuretic Peptide Induces Mitogen-Activated Protein Kinase Phosphatase-1 in Human Endothelial Cells via Rac1 and NAD(P)H Oxidase/Nox2-Activation Circ. Res., January 7, 2005; 96(1): 43 - 53. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-J. Jeong, H.-J. Na, S.-H. Hong, and H.-M. Kim Inhibition of the Stem Cell Factor-Induced Migration of Mast Cells by Dexamethasone Endocrinology, September 1, 2003; 144(9): 4080 - 4086. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Stockand New ideas about aldosterone signaling in epithelia Am J Physiol Renal Physiol, April 1, 2002; 282(4): F559 - F576. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Tsiani, P. Lekas, I. G. Fantus, J. Dlugosz, and C. Whiteside High glucose-enhanced activation of mesangial cell p38 MAPK by ET-1, ANG II, and platelet-derived growth factor Am J Physiol Endocrinol Metab, January 1, 2002; 282(1): E161 - E169. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Touyz and E. L. Schiffrin Signal Transduction Mechanisms Mediating the Physiological and Pathophysiological Actions of Angiotensin II in Vascular Smooth Muscle Cells Pharmacol. Rev., December 1, 2000; 52(4): 639 - 672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Dlugosz, S. Munk, J. Kapor-Drezgic, H. J. Goldberg, I. G. Fantus, J. W. Scholey, and C. I. Whiteside Stretch-induced mesangial cell ERK1/ERK2 activation is enhanced in high glucose by decreased dephosphorylation Am J Physiol Renal Physiol, October 1, 2000; 279(4): F688 - F697. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. KIROYCHEVA, F. AHMED, G. M. ANTHONY, C. SZABO, G. J. SOUTHAN, and N. BANK Mitogen-Activated Protein Kinase Phosphorylation in Kidneys of {beta}s Sickle Cell Mice J. Am. Soc. Nephrol., June 1, 2000; 11(6): 1026 - 1032. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Eguchi, H. Iwasaki, H. Ueno, G. D. Frank, E. D. Motley, K. Eguchi, F. Marumo, Y. Hirata, and T. Inagami Intracellular Signaling of Angiotensin II-induced p70 S6 Kinase Phosphorylation at Ser411 in Vascular Smooth Muscle Cells. POSSIBLE REQUIREMENT OF EPIDERMAL GROWTH FACTOR RECEPTOR, RAS, EXTRACELLULAR SIGNAL-REGULATED KINASE, AND AKT J. Biol. Chem., December 24, 1999; 274(52): 36843 - 36851. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, Y. Hu, M. Mayr, and Q. Xu Cyclic Strain Stress-induced Mitogen-activated Protein Kinase (MAPK) Phosphatase 1 Expression in Vascular Smooth Muscle Cells Is Regulated by Ras/Rac-MAPK Pathways J. Biol. Chem., September 3, 1999; 274(36): 25273 - 25280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Takeda, T. Ichiki, T. Tokunou, N. Iino, and A. Takeshita 15-Deoxy-Delta 12,14-prostaglandin J2 and Thiazolidinediones Activate the MEK/ERK Pathway through Phosphatidylinositol 3-Kinase in Vascular Smooth Muscle Cells J. Biol. Chem., December 21, 2001; 276(52): 48950 - 48955. [Abstract] [Full Text] [PDF]
|
|