(Hypertension. 1999;33:1175-1178.)
© 1999 American Heart Association, Inc.
Scientific Contributions |
The 825C/T Polymorphism of the G-Protein Subunit ß3 Is Not Related to Hypertension
From the Institut National de la Santé et de la Recherche Médicale (INSERM) U525, Paris, France (E.B., S.-M.H., F.C.); INSERM U258, Hôpital Broussais, Paris, France (V.N., L.T.); MONICA Project, Toulouse, France (J.-B.R.); MONICA Project, Belfast, UK (A.E.); MONICA Project, Strasbourg, France (D.A.); MONICA Project, Lille, France (G.L.); and the Hypertension Department (P.-F.P.), Hôpital Broussais, Paris, France.
Correspondence to Dr F. Cambien, Institut National de la Santé et de la Recherche Médicale (INSERM) U525, 17, rue du Fer à Moulin, 75005 Paris, France. E-mail cambien@zedat.fu-berlin.de or herrmann{at}idf.inserm.fr
Abstract—A polymorphism at
position 825 (C
T) of the cDNA that encodes the ß3 subunit (GNB3)
of the pertussis toxin–sensitive G protein was recently shown to be
associated with human hypertension. To verify this finding and to
investigate whether this polymorphism could also be associated with
coronary heart disease, we analyzed the GNB3 variant in
subjects from 2 previously described studies: Projet d'Etude des
Gènes de l'hypertension Artérielle Sévère
à modérée Essentielle (PEGASE), a case-control study
of moderate to severe hypertension (681 cases and 308 controls), and
Etude Cas-Témoins de l'Infarctus du Myocarde (ECTIM), a
case-control study of myocardial infarction (MI) (564 cases and 633
controls). Genotyping was performed with allele-specific
oligonucleotides. Genotype and allele
frequencies were in Hardy-Weinberg equilibrium in all groups.
Allele and genotype frequencies did not differ
significantly between case patients with essential hypertension or MI
and control subjects. In the ECTIM study, the 825T allele
frequencies in cases and controls from Belfast, Northern Ireland, were
0.31 and 0.30 (P=0.79), respectively; the corresponding
frequencies in cases and controls from France were 0.33 and 0.31
(P=0.30), respectively. In the PEGASE study, the 825T
allele frequency was 0.35 in female and male cases and 0.31 in male
normotensive controls (P=0.12). The odds ratios for
hypertension (PEGASE) and MI (ECTIM) associated with T-allele
carrying were 1.23 (95% confidence interval, 0.94 to 1.62;
P=0.13) and 1.11 (95% confidence interval, 0.88 to
1.39; P=0.37), respectively. There was no association of
the GNB3 polymorphism with early onset of hypertension, familial
history of hypertension, or blood pressure level. We conclude that the
825C/T polymorphism of the GNB3 gene did not contribute in any
important way to the risk of essential hypertension or MI in these
studies.
Key Words: hypertension, essential • myocardial infarction • coronary artery stenosis • body mass index • G-protein • C825T polymorphism
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