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(Hypertension. 2000;35:648.)
© 2000 American Heart Association, Inc.

Scientific Contributions

Elevated Perfusion Pressure Upregulates Endothelin-1 and Endothelin B Receptor Expression in the Rabbit Carotid Artery

Manfred Lauth; Marc-Moritz Berger; Marco Cattaruzza; Markus Hecker

From the Department of Cardiovascular Physiology, University of Goettingen, Germany.

Correspondence to Markus Hecker, PhD, Department of Cardiovascular Physiology, University of Goettingen, Humboldtallee 23, 37073 Goettingen, Germany. E-mail hecker{at}veg-physiol.med.uni-goettingen.de

Abstract—To investigate the hypothesis that high blood pressure activates the endothelin system in the vessel wall, isolated segments of the rabbit carotid artery were subjected to different levels of perfusion pressure. Both preproendothelin-1 (ppET-1) mRNA abundance and intravascular ET-1 peptide content were strongly upregulated on raising the intraluminal pressure from 90 to 160 mm Hg for 3 to 12 hours, and this increase in ppET-1 mRNA occurred predominantly in the endothelial cells. Endothelin-converting enzyme-1 and endothelin A receptor (ET_A-R) expression were pressure-insensitive, whereas that of the ET_B-R in the smooth muscle cells was also significantly enhanced. Both the pressure-induced increase in ppET-1 and ET_B-R expression required RNA synthesis because they were abolished by actinomycin D. The nuclear signaling mechanisms involved therein, however, appeared to be different. Thus, the pressure-induced expression of ppET-1 and activation of CCAAT-enhancer binding proteins ß and were blocked by the tyrosine kinase inhibitor herbimycin A, whereas ET_B-R expression and the nuclear translocation of activator protein-1 were abolished by the protein kinase C inhibitor Ro 31-8220. One consequence of these presumably deformation-induced changes in gene expression was an increased rate of apoptosis of the smooth muscle cells in the media that if transferable to the situation in human blood vessels may contribute to hypertension-induced arterial remodeling.

Key Words: hypertension, arterial • endothelin • receptors • genes • carotid arteries

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