(Hypertension. 2000;36:371.)
© 2000 American Heart Association, Inc.
Scientific Contributions |
ß2-Adrenoceptor Polymorphism Determines Vascular Reactivity in Humans
From the School of Medical and Surgical Sciences, Division of Therapeutics, and the Research Centre for Human and Clinical Sciences (J.P.N.), University of Nottingham Medical School, Queen’s Medical Centre, Nottingham, UK.
Correspondence to Dr J.R. Cockcroft, Department of Cardiology, University of Wales College of Medicine, University Hospital, Heath Park, Cardiff CF4 4XN, UK. E-mail cockcroftjr{at}cardiff.ac.uk
Abstract—Altered ß-adrenergic regulation has been reported in individuals with hypertension. The variability in vascular responsiveness to ß-agonists, such as isoproterenol, observed in humans may be explained partially by ß2-adrenoceptor polymorphism. Individuals with the Gln27 form of the receptor may show reduced vascular reactivity because of downregulation expression of the receptor in the vasculature. We screened 127 normotensive white subjects, 37 of whom were homozygous for these alleles. Thirty-two subjects (17 Gln27 and 15 Glu27) agreed to receive brachial artery infusions of isoproterenol at doses of 1 to 300 ng · min-1; forearm blood flow was measured by using venous occlusion plethysmography. Of these subjects, 25 (12 Glu27 and 13 Gln27) received local doses of isoproterenol (0.3 to 30.0 ng · min-1) via a dorsal hand vein preconstricted with norepinephrine. Compared with subjects homozygous for the Glu27 allele, subjects with the Gln27 substitution had lower baseline blood flow and, in response to isoproterenol, had a significantly attenuated increase in forearm blood flow. This pattern was more marked in veins. We also studied the relationship between the position 16 polymorphism and vascular reactivity. Homozygotes for Arg16 had significantly lower basal blood flow and attenuated increases in forearm blood flow compared with the Gly16 homozygotes. This was significant in veins but not in arteries. Thus, ß2-adrenoceptor genotype determines vascular responses to isoproterenol in forearm resistance vessels and in capacitance vessels. Further studies are necessary to establish whether ß2-adrenoceptor polymorphisms are important in the genesis of hypertension.
Key Words: receptors, adrenergic, beta • blood flow • veins • isoproterenol • genes
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