This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zagato, L. Articles by Tripodi, G. Search for Related Content PubMed PubMed Citation Articles by Zagato, L. Articles by Tripodi, G. Pubmed/NCBI databases Gene UniSTS Substance via MeSH Medline Plus Health Information High Blood Pressure Related Collections Genetics of cardiovascular disease

(Hypertension. 2000;36:734.)
© 2000 American Heart Association, Inc.

Scientific Contributions

Genetic Mapping of Blood Pressure Quantitative Trait Loci in Milan Hypertensive Rats

Laura Zagato; Rossana Modica; Monica Florio; Lucia Torielli; Marie-Thérèse Bihoreau; Giuseppe Bianchi; Grazia Tripodi

From the Division of Nephrology (L.Z., G.B.), Dialysis and Hypertension, S. Raffaele Hospital, Milan, Italy; Prassis-Sigma Tau Research Institute (R.M., M.F., L.T., G.T.), Settimo Milanese, Milan, Italy; and The Wellcome Trust Centre for Human Genetics (M.-T.B.), University of Oxford, Oxford, UK.

Correspondence to Grazia Tripodi, PhD, Prassis-Sigma Tau Research Institute, via Forlanini 1, 20019 Settimo Milanese, Milano, Italy. E-mail pstbio{at}tin.it

Abstract—In a previous study, by using a candidate gene approach, we detected in both Milan hypertensive rats and humans a polymorphism in the -adducin gene (ADD1) that was associated with blood pressure and renal sodium handling. In the present study, a genomewide search with 264 informative markers was undertaken in 251 (Milan hypertensive strain x Milan normotensive strain) F2 rats to further investigate the contribution of the adducin gene family (Add1, Add2, and Add3) and to identify novel quantitative trait loci (QTLs) that affect blood pressure. The influence of 2 different methods of blood pressure measurement, the intracarotid catheter and the tail-cuff method, was also evaluated. We found evidence that QTLs affected systolic blood pressure (SBP) measured at the carotid (direct SBP) on rat chromosome 1 with a logarithm of the odds (LOD) score peak of 3.3 on D1Rat121 and on rat chromosome 14 on Add1 locus (LOD=3.2). A QTL for SBP measured at the tail (indirect SBP) was found on rat chromosome 10 around D10Rat33 (LOD=5.0). All of these QTLs identified chromosomal regions not detected in other rat studies and harbor genes (Na⁺/H⁺ exchanger A3; -adducin; _1B-adrenergic receptor) that may be involved in blood pressure regulation. Therefore, these findings may be relevant to human hypertension, also in consideration of the biochemical and pathophysiological similarities between MHS and a subgroup of patients of primary hypertension, which led to the identification of -adducin as a candidate gene in both species.

Key Words: genes • rats, inbred strains • hypertension, essential

This article has been cited by other articles:

				M. Packard, Y. Saad, W. T. Gunning, S. Gupta, J. Shapiro, and M. R. Garrett Investigating the effect of genetic background on proteinuria and renal injury using two hypertensive strains Am J Physiol Renal Physiol, April 1, 2009; 296(4): F839 - F846. [Abstract] [Full Text] [PDF]

				D. L. Mattson, M. R. Dwinell, A. S. Greene, A. E. Kwitek, R. J. Roman, A. W. Cowley Jr., and H. J. Jacob Chromosomal mapping of the genetic basis of hypertension and renal disease in FHH rats Am J Physiol Renal Physiol, December 1, 2007; 293(6): F1905 - F1914. [Abstract] [Full Text] [PDF]

				Y. Saad, S. Yerga-Woolwine, J. Saikumar, P. Farms, E. Manickavasagam, E. J. Toland, and B. Joe Congenic Interval Mapping of RNO10 Reveals a Complex Cluster of Closely-Linked Genetic Determinants of Blood Pressure Hypertension, November 1, 2007; 50(5): 891 - 898. [Abstract] [Full Text] [PDF]

				G. Bianchi Genetic variations of tubular sodium reabsorption leading to "primary" hypertension: from gene polymorphism to clinical symptoms Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2005; 289(6): R1536 - R1549. [Abstract] [Full Text] [PDF]

				P. Meneton, X. Jeunemaitre, H. E. de Wardener, and G. A. Macgregor Links Between Dietary Salt Intake, Renal Salt Handling, Blood Pressure, and Cardiovascular Diseases Physiol Rev, April 1, 2005; 85(2): 679 - 715. [Abstract] [Full Text] [PDF]

				G. Bianchi, P. Ferrari, and J. A. Staessen Adducin Polymorphism: Detection and Impact on Hypertension and Related Disorders Hypertension, March 1, 2005; 45(3): 331 - 340. [Abstract] [Full Text] [PDF]

				A. C.M. Bonne, M. G. den Bieman, G. F. Gillissen, A. Lankhorst, C. J. Kenyon, B. F.M. van Zutphen, and H. A. van Lith Quantitative Trait Loci Influencing Blood and Liver Cholesterol Concentration in Rats Arterioscler Thromb Vasc Biol, December 1, 2002; 22(12): 2072 - 2079. [Abstract] [Full Text] [PDF]

				H. Yang, S. C. Francis, K. Sellers, M. DeBarros, C. Sun, C. Sumners, C. M. Ferrario, M. J. Katovich, A. F. Muro, and M. K. Raizada Hypertension-Linked Decrease in the Expression of Brain {gamma}-Adducin Circ. Res., October 4, 2002; 91(7): 633 - 639. [Abstract] [Full Text] [PDF]

				T. Rice, T. Rankinen, Y. C. Chagnon, M. A. Province, L. Perusse, A. S. Leon, J. S. Skinner, J. H. Wilmore, C. Bouchard, and D. C. Rao Genomewide Linkage Scan of Resting Blood Pressure: HERITAGE Family Study Hypertension, June 1, 2002; 39(6): 1037 - 1043. [Abstract] [Full Text] [PDF]

				S. A. Lloyd-MacGilp, L. Torielli, S. Bechtel, G. Tripodi, C. E. Gomez-Sanchez, L. Zagato, R. Bernhardt, and C. J. Kenyon Mutations in aldosterone synthase gene of Milan hypertensive rats: phenotypic consequences Am J Physiol Endocrinol Metab, March 1, 2002; 282(3): E608 - E617. [Abstract] [Full Text] [PDF]