(Hypertension. 2001;37:630.)
© 2001 American Heart Association, Inc.
Scientific Contributions |
Superoxide Inhibits Neuronal Nitric Oxide Synthase Influences on Afferent Arterioles in Spontaneously Hypertensive Rats
From the Internal Medicine, Keio University School of Medicine, Tokyo, Japan.
Correspondence to Dr Takao Saruta, Department of Internal Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan. E-mail saruta{at}med.keio.ac.jp
This
study was designed to determine the influence of increased superoxide
anion in neuronal nitric oxide synthase (nNOS)-dependent regulation of
afferent arterioles in spontaneously hypertensive rats (SHR). Afferent
arteriolar diameters of male Wistar-Kyoto rats (WKY) and SHR were
assessed in vitro with the blood-perfused juxtamedullary nephron
technique and averaged 21.6±1.6 (n=6) and 18.8±1.2 (n=7) µm,
respectively. The superoxide dismutase mimetic Tempol (1, 10, and 100
µmol/L) did not influence afferent arterioles of WKY but
significantly increased afferent arteriolar diameters of SHR by
20.6±5.5%, 25.2±5.4%, and 23.3±4.9%, respectively. In WKY (n=6),
the nNOS inhibitor
S-methyl-L-thiocitrulline
(L-SMTC; 10 µmol/L) and the NOS inhibitor
N
-nitro-L-arginine
(L-NNA; 100 µmol/L) significantly decreased afferent arteriolar
diameters (19.6±1.6 µm) by 11.9±3.1% and 21.0±3.9%,
respectively. In SHR (n=7), L-SMTC did not influence afferent
arteriolar diameters (21.0±1.5 µm), but L-NNA exerted an afferent
arteriolar constriction (14.8±3.2%) that was similar to the response
observed in WKY. Experiments were also performed in the presence of 100
µmol/L Tempol. In afferent arterioles of WKY (n=6), Tempol treatment
did not modulate the basal diameters (21.5±1.2 µm) or the
constrictor response to L-SMTC (10.6±2.1%) or L-NNA (19.3±3.3%). In
SHR (n=8), Tempol significantly increased afferent arteriolar diameters
by 22.5±4.3% and enhanced afferent arteriolar constrictor responses
to L-SMTC (18.4±2.7%) and L-NNA (31.9±2.6%). However, the nitric
oxide donor
S-nitroso-N-acetylpenicillamine
(10 µmol/L), which similarly increased afferent arteriolar diameters
(17.2±2.3%, n=6), did not affect afferent arteriolar responses to
L-SMTC (1.5±2.7%) or L-NNA (18.6±2.3%). These suggest that
superoxide anion inhibits the control of afferent arteriolar diameters
by nNOS in SHR.
Key Words: Tempol • nitric oxide synthase • arterioles • rats, spontaneously hypertensive • kidney
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