This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mori, T. Articles by Cowley, A. W. Search for Related Content PubMed PubMed Citation Articles by Mori, T. Articles by Cowley, A. W., Jr Pubmed/NCBI databases Gene GEO Profiles HomoloGene UniGene Compound via MeSH Substance via MeSH Hazardous Substances DB CALCIUM COMPOUNDS CALCIUM, ELEMENTAL NITRIC OXIDE Related Collections Cardio-renal physiology/pathophysiology Calcium cycling/excitation-contraction coupling Cell signalling/signal transduction Hypertension - basic studies Imaging Ion channels/membrane transport Endothelium/vascular type/nitric oxide

(Hypertension. 2002;39:465.)
© 2002 American Heart Association, Inc.

Scientific Contributions

Vasopressin Increases Intracellular NO Concentration via Ca²⁺ Signaling in Inner Medullary Collecting Duct

Takefumi Mori; Jeffrey G. Dickhout; Allen W. Cowley, Jr

From the Department of Physiology, Medical College of Wisconsin, Milwaukee.

Correspondence to Takefumi Mori, MD, PhD, Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226. E-mail tmori{at}mcw.edu

The present study was designed to determine whether arginine vasopressin (AVP) stimulates NO production in the epithelial collecting duct cells of the inner medulla (IMCDs) and if this is mediated through Ca²⁺ signaling. Thin tissue layers containing IMCDs were dissected from Sprague-Dawley rats. Intracellular Ca²⁺ concentration ([Ca²⁺]_i) and NO production were measured in IMCDs by a fluorescence imaging system with the use of fura 2-AM and the cell-permeable form of the NO-sensitive dye 4,5-diaminofluorescein (DAF-2), respectively. AVP (100 nmol/L) produced a rapid peak increase in [Ca²⁺]_i of 320±70 nmol/L within a few seconds and a sustained increase of 120±62 nmol/L. The peak increase in [Ca²⁺]_i was followed by a significant increase of NO production (34±7 U). This was similar to that produced by 20 µmol/L of the NO donor DETA-NONOate (42±11 U). The NO scavenger carboxy-PTIO (100 µmol/L) or depletion of [Ca²⁺]_i by preincubation with 5 µmol/L of the Ca²⁺-ATPase inhibitor thapsigargin in Ca²⁺-free buffer abolished the NO response to AVP. We conclude that AVP mobilizes Ca²⁺ to produce NO in IMCDs.

Key Words: vasopressins • nitric oxide • calcium • kidney • signal transduction

This article has been cited by other articles:

				P. M. O'Connor and A. W. Cowley Jr. Vasopressin-induced nitric oxide production in rat inner medullary collecting duct is dependent on V2 receptor activation of the phosphoinositide pathway Am J Physiol Renal Physiol, August 1, 2007; 293(2): F526 - F532. [Abstract] [Full Text] [PDF]

				V. Bachteeva, E. Fock, E. Lavrova, S. Nikolaeva, S. Gambaryan, and R. Parnova Prostaglandin E2 inhibits vasotocin-induced osmotic water permeability in the frog urinary bladder by EP1-receptor-mediated activation of NO/cGMP pathway Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2007; 293(1): R528 - R537. [Abstract] [Full Text] [PDF]

				T. Mori, P. M. O'Connor, M. Abe, and A. W. Cowley Jr Enhanced Superoxide Production in Renal Outer Medulla of Dahl Salt-Sensitive Rats Reduces Nitric Oxide Tubular-Vascular Cross-Talk Hypertension, June 1, 2007; 49(6): 1336 - 1341. [Abstract] [Full Text] [PDF]

				J. G. Dickhout, G. S. Hossain, L. M. Pozza, J. Zhou, S. Lhotak, and R. C. Austin Peroxynitrite Causes Endoplasmic Reticulum Stress and Apoptosis in Human Vascular Endothelium: Implications in Atherogenesis Arterioscler. Thromb. Vasc. Biol., December 1, 2005; 25(12): 2623 - 2629. [Abstract] [Full Text] [PDF]

				M. Herrera and J. L. Garvin Recent Advances in the Regulation of Nitric Oxide in the Kidney Hypertension, June 1, 2005; 45(6): 1062 - 1067. [Abstract] [Full Text] [PDF]

				B. W. M. van Balkom, J. D. Hoffert, C.-L. Chou, and M. A. Knepper Proteomic analysis of long-term vasopressin action in the inner medullary collecting duct of the Brattleboro rat Am J Physiol Renal Physiol, February 1, 2004; 286(2): F216 - F224. [Abstract] [Full Text] [PDF]

				T. Mori and A. W. Cowley Jr. Renal Oxidative Stress in Medullary Thick Ascending Limbs Produced by Elevated NaCl and Glucose Hypertension, February 1, 2004; 43(2): 341 - 346. [Abstract] [Full Text] [PDF]

				T. Mori and A. W. Cowley Jr Angiotensin II-NAD(P)H Oxidase-Stimulated Superoxide Modifies Tubulovascular Nitric Oxide Cross-Talk in Renal Outer Medulla Hypertension, October 1, 2003; 42(4): 588 - 593. [Abstract] [Full Text] [PDF]

				A. W. Cowley Jr., T. Mori, D. Mattson, and A.-P. Zou Role of renal NO production in the regulation of medullary blood flow Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2003; 284(6): R1355 - R1369. [Abstract] [Full Text] [PDF]

				P. A. Ortiz and J. L. Garvin Cardiovascular and renal control in NOS-deficient mouse models Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2003; 284(3): R628 - R638. [Abstract] [Full Text] [PDF]

				S. Combet, N. Geffroy, V. Berthonaud, B. Dick, L. Teillet, J.-M. Verbavatz, B. Corman, and M.-M. Trinh-Trang-Tan Correction of age-related polyuria by dDAVP: molecular analysis of aquaporins and urea transporters Am J Physiol Renal Physiol, January 1, 2003; 284(1): F199 - F208. [Abstract] [Full Text] [PDF]

				F.-X. Yi, A. Y. Zhang, W. B. Campbell, A.-P. Zou, C. van Breemen, and P.-L. Li Simultaneous in situ monitoring of intracellular Ca2+ and NO in endothelium of coronary arteries Am J Physiol Heart Circ Physiol, December 1, 2002; 283(6): H2725 - H2732. [Abstract] [Full Text] [PDF]

				J. G. Dickhout, T. Mori, and A. W. Cowley Jr Tubulovascular Nitric Oxide Crosstalk: Buffering of Angiotensin II-Induced Medullary Vasoconstriction Circ. Res., September 20, 2002; 91(6): 487 - 493. [Abstract] [Full Text] [PDF]