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(Hypertension. 2002;39:470.)
© 2002 American Heart Association, Inc.

Scientific Contributions

Targeted Proteomic Profiling of Renal Na⁺ Transporter and Channel Abundances in Angiotensin II Type 1a Receptor Knockout Mice

Heddwen L. Brooks; Alicia J. Allred; Kathleen T. Beutler; Thomas M. Coffman; Mark A. Knepper

From the Laboratory of Kidney and Electrolyte Metabolism (H.L.B., K.T.B., M.A.K.), National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Md, and the Department of Medicine (A.J.A., T.M.C.), Duke University, Durham, NC.

Correspondence to Mark A. Knepper, MD, PhD, Building 10, Room 6N260, 10 Center Dr, MSC-1603, National Institutes of Health, Bethesda, MD 20892-1603. E-mail knep{at}helix.nih.gov

The renal tubule transporters responsible for Na⁺ and water transport along the nephron have been identified and cloned, permitting comprehensive analysis of transporter protein abundance changes in complex physiological models by using a "targeted proteomics" approach. Here, we apply this approach to screen renal homogenates from mice in which the gene for the angiotensin II type 1a (AT_1a) receptor has been deleted (versus wild-type mice) to determine which sodium transporters and channels are regulated by the AT_1a receptor at the protein abundance level. In mice maintained on a low NaCl diet (<0.02% NaCl), (1) the abundances of 2 aldosterone-regulated transporters were markedly decreased in knockout versus wild-type mice, namely, the thiazide-sensitive cotransporter and the -subunit of the amiloride-sensitive Na⁺ channel (-ENaC); (2) the abundances of ß-ENaC and -ENaC were markedly increased; and (3) there were no significant changes in the abundances of the proximal tubule Na+-H⁺ exchanger or the Na⁺-K⁺-2Cl^- cotransporter of the thick ascending limb. When the experiment was repeated on higher NaCl diets (0.4% or 6% NaCl), the decrease in -ENaC abundance persisted, whereas the other changes were abolished. Analysis of serum aldosterone concentration in AT_1a knockout mice and wild-type mice on the low NaCl diet revealed the absence of a decrease with AT_1a gene deletion (11.8±2.3 nmol/L for knockout mice and 5.7±0.8 nmol/L for wild-type mice [significantly increased]). These results reveal that the AT_1a receptor plays an important role in regulation of Na⁺ transporter and channel proteins in the "post-macula densa" region of the renal tubule via a mechanism that is not dependent on altered circulating aldosterone concentrations.

Key Words: sodium • angiotensin II • receptors, angiotensin II • kidney • mice

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