This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Plunkett, W. C. Articles by Buckalew, V. M. Search for Related Content PubMed PubMed Citation Articles by Plunkett, W. C. Articles by Buckalew, V. M., Jr

Hypertension, Vol 4, 581-589, Copyright © 1982 by American Heart Association

ARTICLES

Evidence for a vascular sensitizing factor in plasma of saline-loaded dogs

WC Plunkett, PM Hutchins, KA Gruber and VM Buckalew Jr

This study investigates whether plasma extracts previously demonstrated to have natriuretic and antinatriferic activity have effects on vascular reactivity of rat cremaster arterioles. Plasma from hydropenic and saline-loaded dogs was subjected to Diafiltration, and eluted on a strong cation exchange column (SCX). The effects of intraarterial injections of various column fractions on constrictor responses to repeated injections of 33.3 ng of norepinephrine (NE) were used to indicate changes in vascular responsiveness in third order cremaster arterioles. SCX fraction I (void volume) from saline-loaded dogs (FI-S) caused an increase in constrictor response to NE of 101%. Increased vascular responsiveness peaked at 40 minutes and remained significantly elevated (p less than 0.05) for 130 minutes. Fraction I from plasma of hydropenic dogs (FI-H) and fraction III from plasma of saline-loaded dogs (FIII-S) did not increase vascular responsiveness to NE. FI-S shifted the dose response curves for NE, arginine vasopressin, and angiotensin II parallel and to the left relative to control by a factor of 3.05-, 2.95-, and 5.63-fold, respectively, at the 50% constrictor dose. Systemic injections of FI-S, but not FI-H, caused a 10 mm Hg rise in blood pressure at 50 minutes, and blood pressure was significantly elevated for 30 to 90 minutes after injection (p less than 0.01). These data demonstrate a vascular-sensitizing factor in FI-S. The factor appears in the same chromatographic fraction previously demonstrated to contain natriuretic, antinatriferic, and digoxin-like activity. The correlation of these activities with salt loading suggests they are due to the same substance, which may be the putative natriuretic hormone.

This article has been cited by other articles:

				S. W. Graves, K. E. Markides, and N. K. Hollenberg Application of Supercritical Fluid Chromatography to Characterize a Labile Digitalis-Like Factor Hypertension, December 1, 2000; 36(6): 1059 - 1064. [Abstract] [Full Text] [PDF]

				M. Currie, D. Geller, B. Cole, J. Boylan, W YuSheng, S. Holmberg, and P Needleman Bioactive cardiac substances: potent vasorelaxant activity in mammalian atria Science, July 1, 1983; 221(4605): 71 - 73. [Abstract] [PDF]