This Article Full Text Full Text (PDF) All Versions of this Article: 40/1/54    most recent 01.HYP.0000021780.21830.DDv1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Christoffersen, C. Articles by Nielsen, L. B. Search for Related Content PubMed PubMed Citation Articles by Christoffersen, C. Articles by Nielsen, L. B. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Related Collections Biochemistry and metabolism Animal models of human disease Gene expression Gene regulation Quantitative modeling Glucose intolerance

(Hypertension. 2002;40:54.)
© 2002 American Heart Association, Inc.

Scientific Contributions

Chamber-Dependent Expression of Brain Natriuretic Peptide and Its mRNA in Normal and Diabetic Pig Heart

Christina Christoffersen; Jens P. Goetze; Emil D. Bartels; Marianne O. Larsen; Ulla Ribel; Jens F. Rehfeld; Bidda Rolin; Lars B. Nielsen

From the Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen (C.C., J.P.G., E.D.B., J.F.R., L.B.N.), Copenhagen, Denmark; and Novo Nordisk A/S (M.O.L., U.R., B.R.), Bagsvaerd, Denmark.

Correspondence to Lars B. Nielsen, MD, PhD, DMSc, Department of Clinical Biochemistry KB3011, Rigshospitalet, University of Copenhagen, Denmark. E-mail larsbo{at}rh.dk

Brain natriuretic peptide (BNP) is produced in cardiac myocytes, and increased secretion is closely associated with cardiac dysfunction. However, several fundamental aspects of BNP expression in the myocardium have not yet been resolved. In the present study, we report the presence of a precursor BNP mRNA transcript and a mature BNP mRNA transcript in normal porcine hearts. In normal pigs, the amount of precursor BNP mRNA was similar in atrial and ventricular myocardium, whereas the mature BNP transcript was 10- to 50-fold more abundant in atrial than in ventricular myocardium. Quantitation of proBNP in normal porcine hearts by radioimmunoassay disclosed abundant proBNP in the atria, whereas proBNP was undetectable in the ventricles. Laser confocal microscopy revealed proBNP in secretory granules of atrial but not in the ventricular myocardium of normal pigs. Mild streptozotocin-induced diabetes doubled the expression of BNP mRNA in porcine atrial myocardium (P=0.03), but was without effect on BNP mRNA in the ventricular myocardium. The data suggest that BNP mRNA processing and proBNP storage differ between the atrial and ventricular myocardium. The results also imply that diabetes increases cardiac BNP expression in a chamber-dependent manner.

Key Words: brain natriuretic peptide • diabetes • gene expression • heart

This article has been cited by other articles:

				C. Christoffersen, I. Hunter, A. L. Jensen, and J. P. Goetze Diabetes and the endocrine heart Eur. Heart J., October 2, 2007; 28(20): 2427 - 2429. [Full Text] [PDF]

				M. L. S. Lindegaard, P. Damm, E. R. Mathiesen, and L. B. Nielsen Placental triglyceride accumulation in maternal type 1 diabetes is associated with increased lipase gene expression J. Lipid Res., November 1, 2006; 47(11): 2581 - 2588. [Abstract] [Full Text] [PDF]

				J. P. Goetze, L. Friis-Hansen, J. F. Rehfeld, B. Nilsson, and J. H. Svendsen Atrial secretion of B-type natriuretic peptide Eur. Heart J., July 2, 2006; 27(14): 1648 - 1650. [Abstract] [Full Text] [PDF]

				Y. Shimoni, D. Hunt, K. Chen, T. Emmett, and G. Kargacin Differential autocrine modulation of atrial and ventricular potassium currents and of oxidative stress in diabetic rats Am J Physiol Heart Circ Physiol, May 1, 2006; 290(5): H1879 - H1888. [Abstract] [Full Text] [PDF]

				T. E. H. Christoffersen, M. Aplin, C. C. Strom, S. P. Sheikh, O. Skott, P. K. Busk, S. Haunso, and L. B. Nielsen Increased natriuretic peptide receptor A and C gene expression in rats with pressure-overload cardiac hypertrophy Am J Physiol Heart Circ Physiol, April 1, 2006; 290(4): H1635 - H1641. [Abstract] [Full Text] [PDF]

				E. M. Madsen, M. L. S. Lindegaard, C. B. Andersen, P. Damm, and L. B. Nielsen Human Placenta Secretes Apolipoprotein B-100-containing Lipoproteins J. Biol. Chem., December 31, 2004; 279(53): 55271 - 55276. [Abstract] [Full Text] [PDF]

				J. P. Goetze Biochemistry of Pro-B-Type Natriuretic Peptide-Derived Peptides: The Endocrine Heart Revisited Clin. Chem., September 1, 2004; 50(9): 1503 - 1510. [Abstract] [Full Text] [PDF]

				J. P. Goetze, W. Yongzhong, J. F Rehfeld, E. Jorgensen, and J. Kastrup Coronary angiography transiently increases plasma pro-B-type natriuretic peptide Eur. Heart J., May 1, 2004; 25(9): 759 - 764. [Abstract] [Full Text] [PDF]

				J. P. Goetze, J. Kastrup, and J. F Rehfeld The paradox of increased natriuretic hormones in congestive heart failure patients: Does the endocrine heart also fail in heart failure? Eur. Heart J., August 2, 2003; 24(16): 1471 - 1472. [Full Text] [PDF]