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(Hypertension. 2002;40:342.)
© 2002 American Heart Association, Inc.

Scientific Contributions

Catecholamine-Related Gene Expression Correlates With Blood Pressures in SHR

Valin Reja; Ann K. Goodchild; Paul M. Pilowsky

From the Hypertension and Stroke Research Laboratories, Department of Physiology, University of Sydney, and Department of Neurosurgery, Royal North Shore Hospital, Sydney, Australia.

Correspondence to Associate Professor Paul M. Pilowsky, Hypertension and Stroke Research Laboratories, Ground Floor, Block 3, Royal North Shore Hospital, Sydney, 2065, Australia. E-mail pilowsky{at}med.usyd.edu.au

In this study we aimed to determine whether the levels of gene expression for phenylethanolamine-N-methyltransferase (PNMT), noradrenaline transporter (NAT), _1A-receptor (_1A-R), and _2A-receptor (_2A-R) vary with resting systolic blood pressure in spontaneously hypertensive rats (SHR) compared with normotensive Wistar-Kyoto (WKY) or Sprague-Dawley (SD) rats. Sites examined included central and peripheral regions associated with the control of arterial pressure. Twenty week old SD (n=6), WKY (n=6), and SHR (n=6) were used. Systolic blood pressure was measured using tail cuff plethysmography 2 weeks before tissue extraction. RNA was isolated and reverse-transcribed into cDNA. Gene expression levels were measured, using quantitative real time PCR, relative to the expression of GAPDH. PNMT, NAT, and _1A-R mRNA expression was significantly greater in SHR tissue samples compared with normotensives. In the rostral ventrolateral medulla, PNMT mRNA in SHR was 3 times greater than that in WKY (SHR: 0.82±0.02%; WKY: 0.29±0.02%). The amount of _2A-R mRNA was significantly lower in SHR compared with normotensives. For example, the level of _2A-R mRNA in spinal cord of SHR was 3 times less than that found in WKY (SHR: 1.85±0.04%; WKY: 3.26±0.07%). PNMT, NAT, and _1A-R mRNA levels were positively correlated with systolic blood pressure in all central tissue investigated. Conversely, _2A-R mRNA levels in central sites were negatively correlated with systolic blood pressure. Clearly, a decrease in central _2A-R and an increase in _1A-R is consistent with the elevated blood pressure and sympathetic activity observed in SHR.

Key Words: catecholamines • hypertension, genetic • receptors, adrenergic alpha • renin-angiotensin system • sympathetic nervous system

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