Published online before print November 25, 2002, doi: 10.1161/01.HYP.0000050102.90932.CF
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
(Hypertension. 2003;41:42.)
© 2003 American Heart Association, Inc.
Scientific Contributions |
Urinary Angiotensinogen as an Indicator of Intrarenal Angiotensin Status in Hypertension
From the Department of Physiology, Tulane University Health Sciences Center, New Orleans, La.
Correspondence to Hiroyuki Kobori, MD, PhD, Department of Physiology, Tulane University Health Sciences Center, 1430 Tulane Avenue, SL39, New Orleans, LA 70112-2699. E-mail hkobori{at}tulane.edu
Angiotensin II (AngII) infusions augment renal angiotensinogen mRNA and protein and urinary angiotensinogen excretion (UAGT). Further experiments were performed in 4 groups of rats: normal salt diet with sham operation, NS+Sham, n=6; NS with AngII infusion at 40 ng/min via osmotic minipump, NS+AngII(40), n=9; NS with AngII infusion at 80 ng/min, NS+AngII(80), n=9; high-salt diet with deoxycorticosterone acetate salt pellet (100 mg), HS+DOCA, n=4. These experiments sought to determine whether enhanced UAGT is specifically associated with increased kidney AngII levels or is a nonspecific consequence of the hypertension. Systolic BP (SBP) was significantly increased to 131±2 and 162±2 mm Hg at day 11 in NS+AngII(40) and NS+AngII(80), respectively, compared with NS+Sham (110±1). Regression analysis demonstrated a positive relationship (R=0.49) between SBP and UAGT for NS+Sham (1.1±0.3 nmol AngI/d), NS+AngII(40) (2.5±0.9), and NS+AngII(80) (5.5±1.5). UAGT was also highly correlated (R=0.70) with kidney AngII content for NS+Sham (49±6 fmol/g), NS+AngII(40) (215±49), and NS+AngII(80) (347±47); but not with plasma AngII (R=0.12). HS+DOCA rats also exhibited increased SBP to 134±1 mm Hg, but UAGT (1.4±0.4 nmol AngI/d) and intrarenal AngII content (13±2 fmol/g) were not increased despite the hypertension. Infused human angiotensinogen could not be detected in urine of sham-operated or AngII-infused rats (n=4 each). These data demonstrate that UAGT increases in AngII-dependent hypertension in a dose- and time-dependent manner, but not in hypertension elicited by HS+DOCA. The results support the hypothesis that AngII-dependent hypertension results in elevated intrarenal AngII and angiotensinogen levels, reflected by increased UAGT, which does not occur in an AngII-independent hypertensive model.
Key Words: angiotensin II • angiotensinogen • rats • kidney • urine • sodium, dietary • deoxycorticosterone acetate salt • Western blot
This article has been cited by other articles:
 |
|
 |
|
  D. Zhao, D. M. Seth, and L. G. Navar Enhanced Distal Nephron Sodium Reabsorption in Chronic Angiotensin II-Infused Mice Hypertension, July 1, 2009; 54(1): 120 - 126. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Shao, D. M. Seth, and L. G. Navar Augmentation of endogenous intrarenal angiotensin II levels in Val5-ANG II-infused rats Am J Physiol Renal Physiol, May 1, 2009; 296(5): F1067 - F1071. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kobori, A. B. Alper Jr, R. Shenava, A. Katsurada, T. Saito, N. Ohashi, M. Urushihara, K. Miyata, R. Satou, L. L. Hamm, et al. Urinary Angiotensinogen as a Novel Biomarker of the Intrarenal Renin-Angiotensin System Status in Hypertensive Patients Hypertension, February 1, 2009; 53(2): 344 - 350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ohashi, T. Yamamoto, Y. Huang, T. Misaki, H. Fukasawa, H. Suzuki, A. Togawa, S. Suzuki, Y. Fujigaki, T. Nakagawa, et al. Intrarenal RAS activity and urinary angiotensinogen excretion in anti-thymocyte serum nephritis rats Am J Physiol Renal Physiol, November 1, 2008; 295(5): F1512 - F1518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. I. Diz Lewis K. Dahl Memorial Lecture: The Renin-Angiotensin System and Aging Hypertension, July 1, 2008; 52(1): 37 - 43. [Full Text] [PDF]
|
|
|
|
|
|
M. C. Prieto-Carrasquero, F. T. Botros, J. Pagan, H. Kobori, D. M. Seth, D. E. Casarini, and L. G. Navar Collecting Duct Renin Is Upregulated in Both Kidneys of 2-Kidney, 1-Clip Goldblatt Hypertensive Rats Hypertension, June 1, 2008; 51(6): 1590 - 1596. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kobori, A. Katsurada, K. Miyata, N. Ohashi, R. Satou, T. Saito, Y. Hagiwara, K. Miyashita, and L. G. Navar Determination of plasma and urinary angiotensinogen levels in rodents by newly developed ELISA Am J Physiol Renal Physiol, May 1, 2008; 294(5): F1257 - F1263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Franco, R. Bautista, O. Perez-Mendez, L. Gonzalez, U. Pacheco, L. G. Sanchez-Lozada, J. Santamaria, E. Tapia, R. Monreal, and F. Martinez Renal interstitial adenosine is increased in angiotensin II-induced hypertensive rats Am J Physiol Renal Physiol, January 1, 2008; 294(1): F84 - F92. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Graciano, A. Nishiyama, K. Jackson, D. M. Seth, R. M. Ortiz, M. C. Prieto-Carrasquero, H. Kobori, and L. G. Navar Purinergic receptors contribute to early mesangial cell transformation and renal vessel hypertrophy during angiotensin II-induced hypertension Am J Physiol Renal Physiol, January 1, 2008; 294(1): F161 - F169. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kobori, M. Nangaku, L. G. Navar, and A. Nishiyama The Intrarenal Renin-Angiotensin System: From Physiology to the Pathobiology of Hypertension and Kidney Disease Pharmacol. Rev., September 1, 2007; 59(3): 251 - 287. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Katsurada, Y. Hagiwara, K. Miyashita, R. Satou, K. Miyata, N. Ohashi, L. G. Navar, and H. Kobori Novel sandwich ELISA for human angiotensinogen Am J Physiol Renal Physiol, September 1, 2007; 293(3): F956 - F960. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kobori, Y. Ozawa, R. Satou, A. Katsurada, K. Miyata, N. Ohashi, N. Hase, Y. Suzaki, C. D. Sigmund, and L. G. Navar Kidney-specific enhancement of ANG II stimulates endogenous intrarenal angiotensinogen in gene-targeted mice Am J Physiol Renal Physiol, September 1, 2007; 293(3): F938 - F945. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ozawa and H. Kobori Crucial role of Rho-nuclear factor-{kappa}B axis in angiotensin II-induced renal injury Am J Physiol Renal Physiol, July 1, 2007; 293(1): F100 - F109. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yamamoto, T. Nakagawa, H. Suzuki, N. Ohashi, H. Fukasawa, Y. Fujigaki, A. Kato, Y. Nakamura, F. Suzuki, and A. Hishida Urinary Angiotensinogen as a Marker of Intrarenal Angiotensin II Activity Associated with Deterioration of Renal Function in Patients with Chronic Kidney Disease J. Am. Soc. Nephrol., May 1, 2007; 18(5): 1558 - 1565. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-M. Lalouel and A. Rohrwasser Genetic Susceptibility to Essential Hypertension: Insight From Angiotensinogen Hypertension, March 1, 2007; 49(3): 597 - 603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ozawa, H. Kobori, Y. Suzaki, and L. G. Navar Sustained renal interstitial macrophage infiltration following chronic angiotensin II infusions Am J Physiol Renal Physiol, January 1, 2007; 292(1): F330 - F339. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Haller, J.-K. Park, C. Lindschau, M. Meyer, and J. Menne Intrarenal renin-angiotensin system -- important player of the local milieu Journal of Renin-Angiotensin-Aldosterone System, June 1, 2006; 7(2): 122 - 125. [PDF]
|
|
|
|
|
|
M. C. Prieto-Carrasquero, H. Kobori, Y. Ozawa, A. Gutierrez, D. Seth, and L. G. Navar AT1 receptor-mediated enhancement of collecting duct renin in angiotensin II-dependent hypertensive rats Am J Physiol Renal Physiol, September 1, 2005; 289(3): F632 - F637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kobori, Y. Ozawa, Y. Suzaki, and A. Nishiyama Enhanced Intrarenal Angiotensinogen Contributes to Early Renal Injury in Spontaneously Hypertensive Rats J. Am. Soc. Nephrol., July 1, 2005; 16(7): 2073 - 2080. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. K. Jacobsen Review: Preventing End-Stage Renal Disease in Diabetic Patients -- Dual Blockade of the Renin-Angiotensin System (Part II) Journal of Renin-Angiotensin-Aldosterone System, June 1, 2005; 6(2): 55 - 68. [Abstract] [PDF]
|
|
|
|
|
|
H. Kobori, M. C. Prieto-Carrasquero, Y. Ozawa, and L. G. Navar AT1 Receptor Mediated Augmentation of Intrarenal Angiotensinogen in Angiotensin II-Dependent Hypertension Hypertension, May 1, 2004; 43(5): 1126 - 1132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Nishiyama, H. Kobori, T. Fukui, G.-X. Zhang, L. Yao, M. Rahman, H. Hitomi, H. Kiyomoto, T. Shokoji, S. Kimura, et al. Role of Angiotensin II and Reactive Oxygen Species in Cyclosporine A-Dependent Hypertension Hypertension, October 1, 2003; 42(4): 754 - 760. [Abstract] [Full Text] [PDF]
|
|